Anti-Mucin 5AC抗体[45M1] (ab3649)

Unfortunately, the specific epitope of ab3649 has not been mapped to our knowledge, so we do not know where within the Mucin 5AC protein the antibody binds.

Thank you for getting back to me and for providing some experimental details.

The protocol looks fine to me. It may well be that the antibody has been damaged during storage or shipment.

This antibody has not been tested with Mouse in IHC-P in the Lab but based on these 3 specification, it is suitable for immonohistochemistry on murine samples.

1. Wallace JL et al. Muc-2-Deficient Mice Display a Sex-Specific, COX-2-Related Impairment of Gastric Mucosal Repair. Am J Pathol 178:1126-33 (2011). IHC-P; Mouse. PubMed: 21356364

2. Chen G et al. Foxa2 programs Th2 cell-mediated innate immunity in the developing lung. J Immunol 184:6133-41 (2010). IHC-P; Mouse.

3. Chen G et al. SPDEF is required for mouse pulmonary goblet cell differentiation and regulates a network of genes associated with mucus production. J Clin Invest 119:2914-24 (2009). IHC-P; Mouse. PubMed: 19759516

As our Abpromise indicates, in the event that a product is not functioning in the applications/species cited on the product data sheet (and the problem has been reported within 6 months of purchase) we will happily offer a credit note/refund to the value of the product purchased.

Would you be so kind to confirm the Abcam Order Number or the PON? This information is necessary for me to have to proceed with your enquiry.

I look forward to hearing from you soon.

I am sorry to hear that you have been experiencing problems using this product in the application that you wish.

In order to assess the quality of our products I would ask that you complete a brief questionnaire relating to the application used. Often it is possible to make suggestions that may help resolve problems experienced using a particular product.

As our Abpromise indicates, in the event that a product is not functioning in the applications/species cited on the product data sheet (and the problem has been reported within 6 months of purchase) we will happily offer a credit note/refund to the value of the product purchased.

All our customer feedback, including complaints are monitored weekly by our in house technical support team. If a product is at fault the technical support team will consider removing the product from our catalogue in order to avoid future customer inconvenience.

Could you provide some further details of the protocol used and complete the following form (attached as a word document). It would be much appreciated if you could attach an image to the response.

Thank you for your understanding and co-operation in this matter. I look forward to hearing from you soon and resolving this issue as soon as possible.

Thank you for your reply.

To clarify, the discount I offered is not a free sample, but can be used to get a free antibody on a future order after submitting an Abreview for ab79082.

This antibody was validated using formalin-fixed paraffin embedded tissue, or formaldehyde-fixed frozen tissue. In IHC-P, antigen retrieval was performed using citrate buffer, pH 6 in the microwave. Sections were blocked using BSA or normal serum from the host species of the secondary antibody. The antibody was diluted 1:50 - 1:100 in PBS and incubated for 1 hour at room temperature or overnight at 4C.

We would expect ab3649 and ab79082 to give the same results in all applications and speciesbecause they are the same clone. In very rare instances, the conjugated forms will behave differently, which is why we do not automatically add all of the applications and speciesto every datasheet for the same clone. The tested applications and specieslisted on our datasheets reflect what hasbeen tested using that particular format.

I hope this helps, please let me know if you need any additional information or assistance.

Yes, Sapphire Bioscienceis our distributor in Australia.

DISCOUNT CODE: ***
Expiration date: August 28th, 2012

I am very pleased to hear you would like to accept our offer and test ab79082 in canine. This code will give you: 1 freeprimary antibodybefore the expiration date. To redeem this offer, please submit an Abreview forcanine and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: https://www.abcam.com/Abreviews.

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

Thank you for contacting us.

Ourantibodies to Mucin 5AC that have been validated for use on frozen tissues are primarily mouse monoclonal antibodies raised against the full length protein, for which the epitopes have not been mapped. Additionally, I have been unable to locate a full length canine Mucin 5AC sequence that I can compare to our immunogen sequences. Unfortunately, I am unable to check the homologies for these reasons.

Of the antibodies we currently sell, I would recommend the 45M1 clone: ab3649 or ab79082 (biotinylated). This clone has been shown to react with mouse, rat, rabbit, chicken, cat, human, pig, monkey, and hedgehog. Because of its broad species reactivity, it would likely also react with dog samples.

If you would like to test this antibody, please let me know, and I will be happy to offer you our 100% testing discount program:

https://www.abcam.com/collaborationdiscount

I hope this helps, please let me know if you have any further questions or concerns.