重组Anti-Mucin 5AC抗体[45M1] (ab3649)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human stomach tissue labeling Mucin 5AC with ab3649 at 1/5000 dilution. Heat mediated antigen retrieval was performed using Citrate buffer pH 6 (epitope retrieval solution 1) for 20 minutes. The section was incubated with ab3649 for 30 mins at room temperature. Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Immunocytochemistry analysis of A549 (human lung carcinoma cell line) cells labeling Mucin 5AC with ab3649 at 1/100 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113) at 1/1000 was used as the secondary antibody (green). Cells were counterstained with Anti-beta Tubulin rabbit monoclonal antibody (ab179513) at 1/200 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) (ab150080) at 1/1000 dilution (red). Nuclear DNA was labelled with DAPI (blue).

Negative control 1: ab3649 (Mouse monoclonal antibody to Mucin 5AC at 1/100 dilution) and ab150080 (anti-Rabbit secondary Alexa Fluor^® 594 at 1/1000 dilution)

Negative control 2: ab179513 (Rabbit monoclonal antibody to beta Tubulin at 1/200 dilution) and ab150113 (anti-Mouse secondary Alexa Fluor^® 488 at 1/1000 dilution)

Immunohistochemistry (Frozen sections) analysis of mouse stomach tissue labeling Mucin 5AC with ab3649 at 1/100 dilution. Tissue was fixed with 4% paraformaldehyde and permeabilized with 0.2% Triton X-100. Heat mediated antigen retrieval was performed using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat Anti-Mouse IgG H&L (Alexa Fluor^® 488) (ab150113) was used as the secondary antibody at 1/1000 dilution (green). Nuclei counterstained with DAPI (blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse stomach tissue labeling Mucin 5AC with ab3649 at 1/5000 dilution. Heat mediated antigen retrieval was performed using Citrate buffer pH 6 (epitope retrieval solution 1) for 20 minutes. The section was incubated with ab3649 for 30 mins at room temperature. Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Immunohistochemistry (Frozen sections) analysis of rat stomach tissue labeling Mucin 5AC with ab3649 at 1/100 dilution. Tissue was fixed with 4% paraformaldehyde and permeabilized with 0.2% Triton X-100. Heat mediated antigen retrieval was performed using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat Anti-Mouse IgG H&L (Alexa Fluor^® 488) (ab150113) was used as the secondary antibody at 1/1000 dilution (green). Nuclei counterstained with DAPI (blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat stomach tissue labeling Mucin 5AC with ab3649 at 1/5000 dilution. Heat mediated antigen retrieval was performed using Citrate buffer pH 6 (epitope retrieval solution 1) for 20 minutes. The section was incubated with ab3649 for 30 mins at room temperature. Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument

This image was generated from the hybridoma version of the product.

Immunohistochemical analysis of rat stomach tissue, staining Mucin 5AC with ab3649.

Antigen retrieval was performed via heat mediation in a citrate buffer. Samples were blocked before incubating with primary antibody (1/100) for 1 hour at room temperature. An HRP-conjugated anti-mouse IgG was used as the secondary antibody and staining was detected using DAB.