Anti-Mucin 5AC抗体[45M1] (ab3649)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mucin 5AC antibody [45M1] (ab3649)](https://www.abcam.cn/ps/products/3/ab3649/Images/ab3649-400131-anti-mucin-5ac-antibody-ihc-human-stomach-tissue.JPG "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mucin 5AC antibody [45M1] (ab3649)")
Key features and details
- Mouse monoclonal [45M1] to Mucin 5AC
- Suitable for: Flow Cyt, IHC-P
- Reacts with: Rat, Human
- Isotype: IgG1
概述
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产品名称
Anti-Mucin 5AC抗体[45M1]
参阅全部 Mucin 5AC 一抗 -
描述
小鼠单克隆抗体[45M1] to Mucin 5AC -
宿主
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanIHC-P RatHuman
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免疫原
Tissue, cells or virus corresponding to Mucin 5AC. Prepared from the fluid of human ovarian mucinous cyst.
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阳性对照
- IHC-P: Rat and human stomach and human gastric carcinoma tissue. Flow Cyt: A549 cells.
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常规说明
This product was changed from ascites to tissue culture supernatant on 20th November 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA -
Concentration information loading... -
纯度
Protein A/G purified -
克隆
单克隆 -
克隆编号
45M1 -
骨髓瘤
unknown -
同种型
IgG1 -
轻链类型
kappa -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab3649 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| 应用 | Species |
|---|---|
| Flow Cyt |
Human
|
| IHC-P |
Rat
Human
|
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt |
Use 0.5-1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. Use at 1 - 2 µg for 106 cells for ascites version. |
|
| IHC-P | (3) |
Use a concentration of 1 - 2 µg/ml.
Use at 1/100 dilution for Ascites version |
| 说明 |
|---|
|
Flow Cyt
Use 0.5-1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. Use at 1 - 2 µg for 106 cells for ascites version. |
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IHC-P
Use a concentration of 1 - 2 µg/ml. Use at 1/100 dilution for Ascites version |
靶标
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相关性
Mucins are high molecular weight glycoproteins with 80% carbohydrates and 20% core protein. Gastric Mucin 5AC antigen is found in columnar mucus cells of surface gastric epithelium and in goblet cells of the fetal and precancerous colon but not in normal colon. Resurgence of gastric mucin during colonic carcinogenesis is suggestive of either re-expression of the peptide core of gastric mucin in the adult colon or due to changes in the glycosylation pattern of mucin, which expose the hidden Mucin 5AC antigen. -
细胞定位
Secreted; Cytoplasmic and cell surface -
数据库链接
- Entrez Gene: 4586 Human
- Entrez Gene: 682837 Rat
- Omim: 158373 Human
- SwissProt: P98088 Human
- Unigene: 534332 Human
- Unigene: 558950 Human
- Unigene: 703588 Human
- Unigene: 703728 Human
see all -
别名
- Apomucin antibody
- Apomucin Major Airway Glycoprotein antibody
- Gastric mucin antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mucin 5AC antibody [45M1] (ab3649)
Immunohistochemical analysis of human stomach tissue labeling Mucin 5AC with ab3649 at 2 µg/ml.
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![Flow Cytometry - Anti-Mucin 5AC antibody [45M1] (ab3649)](https://www.abcam.cn/ps/products/3/ab3649/Images/ab3649-8033-anti-mucin-5ac-antibody-45m1-flow-cytometry.jpg)
Flow Cytometry - Anti-Mucin 5AC antibody [45M1] (ab3649)Overlay histogram showing A549 (Human lung carcinoma cell line) cells stained with ab3649 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3649, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in A549 cells fixed with 80% methanol (5 min) used under the same conditions.
Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mucin 5AC antibody [45M1] (ab3649)](https://www.abcam.cn/ps/products/3/ab3649/Images/ab3649-400130-anti-mucin-5ac-antibody-ihc-human-gastric-carcinoma-tissue.JPG)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mucin 5AC antibody [45M1] (ab3649)Immunohistochemical analysis of human gastric carcinoma tissue labeling Mucin 5AC with ab3649 at 2 µg/ml.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mucin 5AC antibody [45M1] (ab3649)](https://www.abcam.cn/ps/products/3/ab3649/Images/ab3649-400129-anti-mucin-5ac-antibody-ihc-mouse-stomach-tissue.JPG)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mucin 5AC antibody [45M1] (ab3649)Immunohistochemical analysis of rat stomach tissue labeling Mucin 5AC with ab3649 at 2 µg/ml.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mucin 5AC antibody [45M1] (ab3649)](https://www.abcam.cn/ps/products/3/ab3649/Images/Mucin-5AC-Primary-antibodies-ab3649-9.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mucin 5AC antibody [45M1] (ab3649)Image from Kikuchi M et al., BMC Gastroenterol. 2010 Jun 18;10:65. doi: 10.1186/1471-230X-10-65.; Fig 5.; 18 June 2010, BMC Gastroenterology 2010, 10:65Immunohistochemical analysis of rat stomach tissue, staining Mucin 5AC with ab3649.
Antigen retrieval was performed via heat mediation in a citrate buffer. Samples were blocked before incubating with primary antibody (1/100) for 1 hour at room temperature. An HRP-conjugated anti-mouse IgG was used as the secondary antibody and staining was detected using DAB.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (65)
ab3649 被引用在 65 文献中.
- Bequignon E et al. Pathogenesis of chronic rhinosinusitis with nasal polyps: role of IL-6 in airway epithelial cell dysfunction. J Transl Med 18:136 (2020). PubMed: 32209102
- Kojima T et al. The Effects of High Molecular Weight Hyaluronic Acid Eye Drop Application in Environmental Dry Eye Stress Model Mice. Int J Mol Sci 21:N/A (2020). PubMed: 32429217
- Veerati PC et al. Airway Epithelial Cell Immunity Is Delayed During Rhinovirus Infection in Asthma and COPD. Front Immunol 11:974 (2020). PubMed: 32499788
- Schlesinger Y et al. Single-cell transcriptomes of pancreatic preinvasive lesions and cancer reveal acinar metaplastic cells' heterogeneity. Nat Commun 11:4516 (2020). PubMed: 32908137
- Fu DJ et al. Gastric squamous-columnar junction contains a large pool of cancer-prone immature osteopontin responsive Lgr5-CD44+ cells. Nat Commun 11:84 (2020). PubMed: 31901081