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Signal Transduction Cytoskeleton / ECM Extracellular Matrix ECM Proteins MUC

Anti-Mucin 5AC抗体[45M1] (ab3649)

  • Datasheet
  • SDS
Reviews (5)Q&A (6)References (65)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mucin 5AC antibody [45M1] (ab3649)
  • Flow Cytometry - Anti-Mucin 5AC antibody [45M1] (ab3649)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mucin 5AC antibody [45M1] (ab3649)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mucin 5AC antibody [45M1] (ab3649)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mucin 5AC antibody [45M1] (ab3649)

Key features and details

  • Mouse monoclonal [45M1] to Mucin 5AC
  • Suitable for: Flow Cyt, IHC-P
  • Reacts with: Rat, Human
  • Isotype: IgG1

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概述

  • 产品名称

    Anti-Mucin 5AC抗体[45M1]
    参阅全部 Mucin 5AC 一抗
  • 描述

    小鼠单克隆抗体[45M1] to Mucin 5AC
  • 宿主

    Mouse
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Rat
    Human
    See all applications and species data
  • 免疫原

    Tissue, cells or virus corresponding to Mucin 5AC. Prepared from the fluid of human ovarian mucinous cyst.

  • 阳性对照

    • IHC-P: Rat and human stomach and human gastric carcinoma tissue. Flow Cyt: A549 cells.
  • 常规说明

    This product was changed from ascites to tissue culture supernatant on 20th November 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液

    pH: 7.2
    Preservative: 0.05% Sodium azide
    Constituents: PBS, 0.05% BSA
  • Concentration information loading...
  • 纯度

    Protein A/G purified
  • 克隆

    单克隆
  • 克隆编号

    45M1
  • 骨髓瘤

    unknown
  • 同种型

    IgG1
  • 轻链类型

    kappa
  • 研究领域

    • Signal Transduction
    • Cytoskeleton / ECM
    • Extracellular Matrix
    • ECM Proteins
    • MUC
    • Cancer
    • Invasion/microenvironment
    • ECM
    • Extracellular matrix
    • MUC
    • Cancer
    • Tumor immunology
    • Tumor-associated antigens
    • Cancer
    • Tumor biomarkers
    • Tumor antigens

相关产品

  • Alternative Versions

    • Anti-Mucin 5AC antibody [45M1] - BSA and Azide free (ab212636)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
    • Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control (ab91353)
  • Recombinant Protein

    • Recombinant Human Mucin 5AC protein (Tagged) (ab114218)

应用

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab3649 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

应用 Species
Flow Cyt
Human
IHC-P
Rat
Human
应用 Ab评论 说明
Flow Cyt
Use 0.5-1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Use at 1 - 2 µg for 106 cells for ascites version.

IHC-P (3)
Use a concentration of 1 - 2 µg/ml.

Use at 1/100 dilution for Ascites version

说明
Flow Cyt
Use 0.5-1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Use at 1 - 2 µg for 106 cells for ascites version.

IHC-P
Use a concentration of 1 - 2 µg/ml.

Use at 1/100 dilution for Ascites version

靶标

  • 相关性

    Mucins are high molecular weight glycoproteins with 80% carbohydrates and 20% core protein. Gastric Mucin 5AC antigen is found in columnar mucus cells of surface gastric epithelium and in goblet cells of the fetal and precancerous colon but not in normal colon. Resurgence of gastric mucin during colonic carcinogenesis is suggestive of either re-expression of the peptide core of gastric mucin in the adult colon or due to changes in the glycosylation pattern of mucin, which expose the hidden Mucin 5AC antigen.
  • 细胞定位

    Secreted; Cytoplasmic and cell surface
  • 数据库链接

    • Entrez Gene: 4586 Human
    • Entrez Gene: 682837 Rat
    • Omim: 158373 Human
    • SwissProt: P98088 Human
    • Unigene: 534332 Human
    • Unigene: 558950 Human
    • Unigene: 703588 Human
    • Unigene: 703728 Human
    • Unigene: 721515 Human
    • Unigene: 10789 Rat
    see all
  • 别名

    • Apomucin antibody
    • Apomucin Major Airway Glycoprotein antibody
    • Gastric mucin antibody
    • LeB antibody
    • Lewis B blood group antigen antibody
    • Major Airway Glycoprotein antibody
    • MUC 5 antibody
    • MUC5 antibody
    • MUC5AC antibody
    • Mucin 5 subtype AC tracheobronchial antibody
    • Mucin 5 Subtypes A And C antibody
    • Mucin 5 subtypes A and C tracheobronchial/gastric antibody
    • Mucin 5, tracheobronchial/gastric antibody
    • Mucin 5AC antibody
    • Mucin 5AC oligomeric mucus/gel forming antibody
    • Mucin 5AC, oligomeric mucus/gel forming pseudogene antibody
    • Mucin antibody
    • TBM antibody
    • Tracheobronchial Mucin (TBM) antibody
    • Tracheobronchial mucin antibody
    see all

图片

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mucin 5AC antibody [45M1] (ab3649)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mucin 5AC antibody [45M1] (ab3649)

    Immunohistochemical analysis of human stomach tissue labeling Mucin 5AC with ab3649 at 2 µg/ml.

  • Flow Cytometry - Anti-Mucin 5AC antibody [45M1] (ab3649)
    Flow Cytometry - Anti-Mucin 5AC antibody [45M1] (ab3649)

    Overlay histogram showing A549 (Human lung carcinoma cell line) cells stained with ab3649 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3649, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in A549 cells fixed with 80% methanol (5 min) used under the same conditions.

    Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mucin 5AC antibody [45M1] (ab3649)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mucin 5AC antibody [45M1] (ab3649)

    Immunohistochemical analysis of human gastric carcinoma tissue labeling Mucin 5AC with ab3649 at 2 µg/ml.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mucin 5AC antibody [45M1] (ab3649)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mucin 5AC antibody [45M1] (ab3649)

    Immunohistochemical analysis of rat stomach tissue labeling Mucin 5AC with ab3649 at 2 µg/ml.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mucin 5AC antibody [45M1] (ab3649)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mucin 5AC antibody [45M1] (ab3649)Image from Kikuchi M et al., BMC Gastroenterol. 2010 Jun 18;10:65. doi: 10.1186/1471-230X-10-65.; Fig 5.; 18 June 2010, BMC Gastroenterology 2010, 10:65

    Immunohistochemical analysis of rat stomach tissue, staining Mucin 5AC with ab3649.

    Antigen retrieval was performed via heat mediation in a citrate buffer. Samples were blocked before incubating with primary antibody (1/100) for 1 hour at room temperature. An HRP-conjugated anti-mouse IgG was used as the secondary antibody and staining was detected using DAB.

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

数据表及文件

  • SDS download

  • Datasheet download

    Download

文献 (65)

发表研究结果有使用 ab3649?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab3649 被引用在 65 文献中.

  • Bequignon E  et al. Pathogenesis of chronic rhinosinusitis with nasal polyps: role of IL-6 in airway epithelial cell dysfunction. J Transl Med 18:136 (2020). PubMed: 32209102
  • Kojima T  et al. The Effects of High Molecular Weight Hyaluronic Acid Eye Drop Application in Environmental Dry Eye Stress Model Mice. Int J Mol Sci 21:N/A (2020). PubMed: 32429217
  • Veerati PC  et al. Airway Epithelial Cell Immunity Is Delayed During Rhinovirus Infection in Asthma and COPD. Front Immunol 11:974 (2020). PubMed: 32499788
  • Schlesinger Y  et al. Single-cell transcriptomes of pancreatic preinvasive lesions and cancer reveal acinar metaplastic cells' heterogeneity. Nat Commun 11:4516 (2020). PubMed: 32908137
  • Fu DJ  et al. Gastric squamous-columnar junction contains a large pool of cancer-prone immature osteopontin responsive Lgr5-CD44+ cells. Nat Commun 11:84 (2020). PubMed: 31901081
View all Publications for this product

客户评价及客户问答

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1-3 of 3 Abreviews or Q&A

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Mucin 5AC antibody [45M1]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Pig Tissue sections (lung)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: TE pH8
Permeabilization
No
Specification
lung
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative
10% normal buffered formalin
Read More

Abcam user community

Verified customer

提交于 Aug 26 2016

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Mucin 5AC antibody [45M1]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (stomach)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: TE pH8
Permeabilization
No
Specification
stomach
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative
10% buffered normal formalin
Read More

Abcam user community

Verified customer

提交于 Aug 22 2016

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Mucin 5AC antibody [45M1]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Lung)
Specification
Lung
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Read More

Abcam user community

Verified customer

提交于 May 15 2012

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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