Key features and details
- Mouse monoclonal [45M1] to Mucin 5AC
- Suitable for: Flow Cyt, IHC-P
- Reacts with: Rat, Human
- Isotype: IgG1
参阅全部 Mucin 5AC 一抗
描述小鼠单克隆抗体[45M1] to Mucin 5AC
经测试应用适用于: Flow Cyt, IHC-Pmore details
种属反应性与反应: Rat, Human
Tissue, cells or virus corresponding to Mucin 5AC. Prepared from the fluid of human ovarian mucinous cyst.
- IHC-P: Rat and human stomach and human gastric carcinoma tissue. Flow Cyt: A549 cells.
This product was changed from ascites to tissue culture supernatant on 20th November 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
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We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA
Concentration information loading...
纯度Protein A/G purified
Our Abpromise guarantee covers the use of ab3649 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 0.5-1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
Use at 1 - 2 µg for 106 cells for ascites version.
|IHC-P||Use a concentration of 1 - 2 µg/ml.
Use at 1/100 dilution for Ascites version
相关性Mucins are high molecular weight glycoproteins with 80% carbohydrates and 20% core protein. Gastric Mucin 5AC antigen is found in columnar mucus cells of surface gastric epithelium and in goblet cells of the fetal and precancerous colon but not in normal colon. Resurgence of gastric mucin during colonic carcinogenesis is suggestive of either re-expression of the peptide core of gastric mucin in the adult colon or due to changes in the glycosylation pattern of mucin, which expose the hidden Mucin 5AC antigen.
细胞定位Secreted; Cytoplasmic and cell surface
- Apomucin antibody
- Apomucin Major Airway Glycoprotein antibody
- Gastric mucin antibody
Immunohistochemical analysis of human stomach tissue labeling Mucin 5AC with ab3649 at 2 µg/ml.
Overlay histogram showing A549 (Human lung carcinoma cell line) cells stained with ab3649 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3649, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in A549 cells fixed with 80% methanol (5 min) used under the same conditions.
Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
Immunohistochemical analysis of human gastric carcinoma tissue labeling Mucin 5AC with ab3649 at 2 µg/ml.
Immunohistochemical analysis of rat stomach tissue labeling Mucin 5AC with ab3649 at 2 µg/ml.
Immunohistochemical analysis of rat stomach tissue, staining Mucin 5AC with ab3649.
Antigen retrieval was performed via heat mediation in a citrate buffer. Samples were blocked before incubating with primary antibody (1/100) for 1 hour at room temperature. An HRP-conjugated anti-mouse IgG was used as the secondary antibody and staining was detected using DAB.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab3649 被引用在 65 文献中.
- Bequignon E et al. Pathogenesis of chronic rhinosinusitis with nasal polyps: role of IL-6 in airway epithelial cell dysfunction. J Transl Med 18:136 (2020). PubMed: 32209102
- Kojima T et al. The Effects of High Molecular Weight Hyaluronic Acid Eye Drop Application in Environmental Dry Eye Stress Model Mice. Int J Mol Sci 21:N/A (2020). PubMed: 32429217
- Veerati PC et al. Airway Epithelial Cell Immunity Is Delayed During Rhinovirus Infection in Asthma and COPD. Front Immunol 11:974 (2020). PubMed: 32499788
- Schlesinger Y et al. Single-cell transcriptomes of pancreatic preinvasive lesions and cancer reveal acinar metaplastic cells' heterogeneity. Nat Commun 11:4516 (2020). PubMed: 32908137
- Fu DJ et al. Gastric squamous-columnar junction contains a large pool of cancer-prone immature osteopontin responsive Lgr5-CD44+ cells. Nat Commun 11:84 (2020). PubMed: 31901081