Anti-Mucin 5AC抗体[2-11M1] (ab24071)
Key features and details
- Mouse monoclonal [2-11M1] to Mucin 5AC
- Suitable for: Flow Cyt
- Reacts with: Human
- Isotype: IgG1
概述
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产品名称
Anti-Mucin 5AC抗体[2-11M1]
参阅全部 Mucin 5AC 一抗 -
描述
小鼠单克隆抗体[2-11M1] to Mucin 5AC -
宿主
Mouse -
经测试应用
适用于: Flow Cytmore details -
种属反应性
与反应: Human -
免疫原
Mucin isolated from an ovarian cyst fluid (pure endocervical type according to the Fenoglio's classification) Human.
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常规说明
This antibody reacts with peptide core of gastric mucin restricted to the surface gastric epithelium. It characterised the M1-b epitope, completely destroyed by b-mercapto-ethano. The M1-b epitope is found located on a 58 kD and 40 kD peptide after trypsin treatment of native M1 mucin. This M1-b epitope coprecipites with the M1-f epitope encoded by Mucin 5AC gene. It is probably associated with the gastric mucin encoded by Mucin 5AC gene or with a gastric mucin encoded by another gastric MUC gene which polymerized with the M1/Mucin 5AC gastric mucin.
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性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
存储溶液
pH: 7.3
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading...
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纯度
Protein G purified -
Primary antibody说明
This antibody reacts with peptide core of gastric mucin restricted to the surface gastric epithelium. It characterised the M1-b epitope, completely destroyed by b-mercapto-ethano. The M1-b epitope is found located on a 58 kD and 40 kD peptide after trypsin treatment of native M1 mucin. This M1-b epitope coprecipites with the M1-f epitope encoded by Mucin 5AC gene. It is probably associated with the gastric mucin encoded by Mucin 5AC gene or with a gastric mucin encoded by another gastric MUC gene which polymerized with the M1/Mucin 5AC gastric mucin. -
克隆
单克隆 -
克隆编号
2-11M1 -
骨髓瘤
Sp2/0 -
同种型
IgG1 -
轻链类型
kappa -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab24071于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Flow Cyt |
Use 2µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
说明 |
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Flow Cyt
Use 2µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
靶标
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功能
Gel-forming glycoprotein of gastric and respiratoy tract epithelia that protects the mucosa from infection and chemical damage by binding to inhaled microrganisms and particles that are subsequently removed by the mucocilary system. -
组织特异性
Highly expressed in surface mucosal cells of respiratory tract and stomach epithelia. Overexpressed in a number of carcinomas. Also expressed in Barrett's esophagus epithelium and in the proximal duodenum. -
序列相似性
Contains 1 CTCK (C-terminal cystine knot-like) domain.
Contains 3 TIL (trypsin inhibitory-like) domains.
Contains 4 VWFC domains.
Contains 4 VWFD domains. -
结构域
The cysteine residues in the Cys-rich subdomain repeats are not involved in disulfide bonding. -
翻译后修饰
C-, O- and N-glycosylated. O-glycosylated on the Thr-/Ser-rich tandem repeats. C-mannosylation in the Cys-rich subdomains may be required for proper folding of these regions and for export from the endoplasmic reticulum during biosynthesis.
Proteolytic cleavage in the C-terminal is initiated early in the secretory pathway and does not involve a serine protease. The extent of cleavage is increased in the acidic parts of the secretory pathway. Cleavage generates a reactive group which could link the protein to a primary amide. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 4586 Human
- Omim: 158373 Human
- SwissProt: P98088 Human
- Unigene: 534332 Human
- Unigene: 558950 Human
- Unigene: 703588 Human
- Unigene: 703728 Human
- Unigene: 721515 Human
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别名
- Apomucin antibody
- Apomucin Major Airway Glycoprotein antibody
- Gastric mucin antibody
see all
图片
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Overlay histogram showing A549 cells stained with ab24071 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab24071, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
实验方案
数据表及文件
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Datasheet download
文献 (24)
ab24071 被引用在 24 文献中.
- Wang Z et al. SARS-CoV-2 treatment effects induced by ACE2-expressing microparticles are explained by the oxidized cholesterol-increased endosomal pH of alveolar macrophages. Cell Mol Immunol 19:210-221 (2022). PubMed: 34983944
- Huang X et al. MUC5B regulates goblet cell differentiation and reduces inflammation in a murine COPD model. Respir Res 23:11 (2022). PubMed: 35042537
- Morgan R et al. A medium composition containing normal resting glucose that supports differentiation of primary human airway cells. Sci Rep 12:1540 (2022). PubMed: 35087167
- Keith JD et al. Muc5b Contributes to Mucus Abnormality in Rat Models of Cystic Fibrosis. Front Physiol 13:884166 (2022). PubMed: 35574458
- Jo WS et al. Low Dose Rate Radiation Regulates M2-like Macrophages in an Allergic Airway Inflammation Mouse Model. Dose Response 20:15593258221117349 (2022). PubMed: 36003321