Anti-MUC2抗体[SPM512] (ab231427)
Key features and details
- Mouse monoclonal [SPM512] to MUC2
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG1
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-MUC2抗体[SPM512]
参阅全部 MUC2 一抗 -
描述
小鼠单克隆抗体[SPM512] to MUC2 -
宿主
Mouse -
经测试应用
适用于: IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to Human MUC2.
Database link: Q02817 -
阳性对照
- IHC-P: Human small intestine, colon, colon adenocarcinoma, prostate adenocarcinoma and stomach adenocarcinoma tissues.
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常规说明
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading...
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纯度
Protein A/G purified -
纯化说明
Purified from TCS by protein A/G. -
克隆
单克隆 -
克隆编号
SPM512 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab231427于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Primary antibody incubation for 30 minutes at room temperature. |
说明 |
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IHC-P
1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Primary antibody incubation for 30 minutes at room temperature. |
靶标
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功能
Coats the epithelia of the intestines, airways, and other mucus membrane-containing organs. Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces. Major constituent of both the inner and outer mucus layers of the colon and may play a role in excluding bacteria from the inner mucus layer. -
组织特异性
Colon, small intestine, colonic tumors, bronchus, cervix and gall bladder. -
序列相似性
Contains 1 CTCK (C-terminal cystine knot-like) domain.
Contains 1 TIL (trypsin inhibitory-like) domain.
Contains 2 VWFC domains.
Contains 4 VWFD domains. -
翻译后修饰
O-glycosylated.
May undergo proteolytic cleavage in the outer mucus layer of the colon, contributing to the expanded volume and loose nature of this layer which allows for bacterial colonization in contrast to the inner mucus layer which is dense and devoid of bacteria.
At low pH of 6 and under, undergoes autocatalytic cleavage in vitro in the N-terminal region of the fourth VWD domain. It is likely that this also occurs in vivo and is triggered by the low pH of the late secretory pathway. -
细胞定位
Secreted. In the intestine, secreted into the inner and outer mucus layers. - Information by UniProt
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数据库链接
- Entrez Gene: 4583 Human
- Omim: 158370 Human
- SwissProt: Q02817 Human
- Unigene: 315 Human
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别名
- Intestinal mucin 2 antibody
- Intestinal mucin-2 antibody
- MLP antibody
see all
图片
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Formalin-fixed, paraffin-embedded human stomach adenocarcinoma tissue stained for MUC2 using ab231427 at 1/200 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human colon adenocarcinoma tissue stained for MUC2 using ab231427 at 1/200 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human colon tissue stained for MUC2 using ab231427 at 1/200 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human prostate adenocarcinoma tissue stained for MUC2 using ab231427 at 1/200 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human small intestine tissue stained for MUC2 using ab231427 at 1/200 dilution in immunohistochemical analysis.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab231427 被引用在 2 文献中.
- Livanos AE et al. Intestinal Host Response to SARS-CoV-2 Infection and COVID-19 Outcomes in Patients With Gastrointestinal Symptoms. Gastroenterology 160:2435-2450.e34 (2021). PubMed: 33676971
- Han L et al. A case report of endoscopic submucosal dissection for a new subtype of gastric adenoma: mixed fundic and pyloric mucosa type. Transl Cancer Res 10:3053-3058 (2021). PubMed: 35116613