Anti-MUC16抗体[OV185:1] (ab697)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC16 antibody [OV185:1] (ab697)

    Key features and details

    • Mouse monoclonal [OV185:1] to MUC16
    • Suitable for: IHC-P
    • Reacts with: Human
    • Isotype: IgG1

    概述

    • 产品名称

      Anti-MUC16抗体[OV185:1]
      参阅全部 MUC16 一抗

    • 描述

      小鼠单克隆抗体[OV185:1] to MUC16

    • 宿主

      Mouse

    • 经测试应用

      适用于: IHC-Pmore details

    • 种属反应性

      与反应: Human

    • 免疫原

      Full length protein corresponding to Human MUC16. Partially purified human mucin fraction from a pool of cancer tissues of patients with epithelial ovarian.

    • 阳性对照

      • Ovarian carcinoma.

    • 常规说明

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab697于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    IHC-P
    1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
    说明
    IHC-P
    1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    靶标

    • 功能

      Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces.

    • 组织特异性

      Expressed in corneal and conjunctival epithelia (at protein level). Overexpressed in ovarian carcinomas and ovarian low malignant potential (LMP) tumors as compared to the expression in normal ovarian tissue and ovarian adenomas.

    • 序列相似性

      Contains 2 ANK repeats.
      Contains 56 SEA domains.

    • 结构域

      Composed of three domains, a Ser-, Thr-rich N-terminal domain, a repeated domain containing more than 60 partially conserved tandem repeats of 156 amino acids each (AAs 12061-21862) and a C-terminal transmembrane contain domain with a short cytoplasmic tail.

    • 翻译后修饰

      Heavily O-glycosylated; expresses both type 1 and type 2 core glycans.
      Heavily N-glycosylated; expresses primarily high mannose and complex bisecting type N-linked glycans.
      May be phosphorylated. Phosphorylation of the intracellular C-terminal domain may induce proteolytic cleavage and the liberation of the extracellular domain into the extracellular space.
      May contain numerous disulfide bridges. Association of several molecules of the secreted form may occur through interchain disulfide bridges providing an extraordinarily large gel-like matrix in the extracellular space or in the lumen of secretory ducts.

    • 细胞定位

      Cell membrane. Secreted > extracellular space. May be liberated into the extracellular space following the phosphorylation of the intracellular C-terminus which induces the proteolytic cleavage and liberation of the extracellular domain.
    • Information by UniProt

    • 数据库链接

      • 别名

        • CA 125 antibody
        • CA-125 antibody
        • CA125 antibody
        • CA125 ovarian cancer antigen antibody
        • Cancer antigen 125 antibody
        • FLJ14303 antibody
        • MUC 16 antibody
        • MUC-16 antibody
        • MUC16 antibody
        • MUC16_HUMAN antibody
        • Mucin 16 antibody
        • Mucin 16 cell surface associated antibody
        • Mucin-16 antibody
        • Mucin16 antibody
        • Ovarian cancer related tumor marker CA125 antibody
        • Ovarian cancer-related tumor marker CA125 antibody
        • Ovarian carcinoma antigen CA125 antibody
        see all

      图片

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC16 antibody [OV185:1] (ab697)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC16 antibody [OV185:1] (ab697)
        Formalin fixed paraffin embedded ovarian cancer tissue, staining MUC16 with ab697 in immunohistochemical analysis.

      文献 (3)

      发表研究结果有使用 ab697？请让我们知道，以便我们可以引用本数据表中的参考文章。

      ab697 被引用在 3 文献中.

      • Shibata S  et al. Selective Laminin-Directed Differentiation of Human Induced Pluripotent Stem Cells into Distinct Ocular Lineages. Cell Rep 25:1668-1679.e5 (2018). PubMed: 30404017
      • Hayashi R  et al. Coordinated generation of multiple ocular-like cell lineages and fabrication of functional corneal epithelial cell sheets from human iPS cells. Nat Protoc 12:683-696 (2017). PubMed: 28253236
      • Kobayashi Y  et al. Generation of a TALEN-mediated, p63 knock-in in human induced pluripotent stem cells. Stem Cell Res 25:256-265 (2017). IF ; Human . PubMed: 29179035

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