Anti-MUC16抗体[OV185:1] (ab697)

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Key features and details

  • Mouse monoclonal [OV185:1] to MUC16
  • Suitable for: Flow Cyt, IHC-P, IHC-Fr
  • Reacts with: Human
  • Isotype: IgG1

概述

  • 产品名称

    Anti-MUC16抗体[OV185:1]
    参阅全部 MUC16 一抗

  • 描述

    小鼠单克隆抗体[OV185:1] to MUC16

  • 宿主

    Mouse

  • 经测试应用

    适用于: Flow Cyt, IHC-P, IHC-Frmore details

  • 种属反应性

    与反应: Human

  • 免疫原

    Partially purified human mucin fraction from a pool of cancer tissues of patients with epithelial ovarian.

  • 阳性对照

    • Ovarian carcinoma.

性能

应用

Our Abpromise guarantee covers the use of ab697 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 
IHC-P Use at an assay dependent concentration. 30 - 60 mins at RT (ABC method). Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining.
IHC-Fr Use at an assay dependent concentration.

靶标

  • 功能

    Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces.

  • 组织特异性

    Expressed in corneal and conjunctival epithelia (at protein level). Overexpressed in ovarian carcinomas and ovarian low malignant potential (LMP) tumors as compared to the expression in normal ovarian tissue and ovarian adenomas.

  • 序列相似性

    Contains 2 ANK repeats.
    Contains 56 SEA domains.

  • 结构域

    Composed of three domains, a Ser-, Thr-rich N-terminal domain, a repeated domain containing more than 60 partially conserved tandem repeats of 156 amino acids each (AAs 12061-21862) and a C-terminal transmembrane contain domain with a short cytoplasmic tail.

  • 翻译后修饰

    Heavily O-glycosylated; expresses both type 1 and type 2 core glycans.
    Heavily N-glycosylated; expresses primarily high mannose and complex bisecting type N-linked glycans.
    May be phosphorylated. Phosphorylation of the intracellular C-terminal domain may induce proteolytic cleavage and the liberation of the extracellular domain into the extracellular space.
    May contain numerous disulfide bridges. Association of several molecules of the secreted form may occur through interchain disulfide bridges providing an extraordinarily large gel-like matrix in the extracellular space or in the lumen of secretory ducts.

  • 细胞定位

    Cell membrane. Secreted > extracellular space. May be liberated into the extracellular space following the phosphorylation of the intracellular C-terminus which induces the proteolytic cleavage and liberation of the extracellular domain.
  • Information by UniProt

  • 数据库链接

    • 别名

      • CA 125 antibody
      • CA-125 antibody
      • CA125 antibody
      • CA125 ovarian cancer antigen antibody
      • Cancer antigen 125 antibody
      • FLJ14303 antibody
      • MUC 16 antibody
      • MUC-16 antibody
      • MUC16 antibody
      • MUC16_HUMAN antibody
      • Mucin 16 antibody
      • Mucin 16 cell surface associated antibody
      • Mucin-16 antibody
      • Mucin16 antibody
      • Ovarian cancer related tumor marker CA125 antibody
      • Ovarian cancer-related tumor marker CA125 antibody
      • Ovarian carcinoma antigen CA125 antibody
      see all

    图片

    • Flow Cytometry - Anti-MUC16 antibody [OV185:1] (ab697)
      Flow Cytometry - Anti-MUC16 antibody [OV185:1] (ab697)
      Overlay histogram showing HeLa cells stained with ab697 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab697, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

      Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

    文献

    发表研究结果有使用 ab697？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab697 被引用在 2 文献中.

    • Shibata S  et al. Selective Laminin-Directed Differentiation of Human Induced Pluripotent Stem Cells into Distinct Ocular Lineages. Cell Rep 25:1668-1679.e5 (2018). PubMed: 30404017
    • Kobayashi Y  et al. Generation of a TALEN-mediated, p63 knock-in in human induced pluripotent stem cells. Stem Cell Res 25:256-265 (2017). IF ; Human . PubMed: 29179035

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