Anti-MUC16抗体[OC125] (ab693)
概述
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产品名称Anti-MUC16抗体[OC125]
参阅全部 MUC16 一抗 -
描述小鼠单克隆抗体[OC125] to MUC16
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宿主Mouse
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特异性Studies have show that this antibody reacts with approximately 80% of epithelial ovarian neoplasms of serous, endometrioid, clear cell and undifferentiated types. No reactivity has been shown for mucinous ovarian tumors or in germ cell or hematopoietic tumors. It reacts with both normal tissues and neoplasms of fallopian tube, endometrium, endocervix and mesothelioma. It does not react with colon cancer. Normal tissues such as breast, liver, skin, kidney and spleen are negative.
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经测试应用适用于: Flow Cyt, IHC-Pmore details
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种属反应性与反应: Human
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免疫原
Partially purified human mucin fraction from a pool of tissues from patients with epithelial ovarian cancer.
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表位This antibody recognizes an epitope on a molecule called Cancer Antigen 125 (CA125).
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阳性对照
- Flow cytometry: HeLa cells. IHC-P: Ovarian cancer tissue.
性能
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形式Liquid
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存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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存储溶液pH: 7.30
Preservative: 0.09% Sodium azide
Constituents: PBS, 1% BSA, Renoir Red diluent -
Concentration information loading... -
克隆单克隆
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克隆编号OC125
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同种型IgG1
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研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Unmasking reagent
应用
Our Abpromise guarantee covers the use of ab693 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
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| Flow Cyt | Use 1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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| IHC-P | 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. ABC Method. |
靶标
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功能Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces.
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组织特异性Expressed in corneal and conjunctival epithelia (at protein level). Overexpressed in ovarian carcinomas and ovarian low malignant potential (LMP) tumors as compared to the expression in normal ovarian tissue and ovarian adenomas.
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序列相似性Contains 2 ANK repeats.
Contains 56 SEA domains. -
结构域Composed of three domains, a Ser-, Thr-rich N-terminal domain, a repeated domain containing more than 60 partially conserved tandem repeats of 156 amino acids each (AAs 12061-21862) and a C-terminal transmembrane contain domain with a short cytoplasmic tail.
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翻译后修饰Heavily O-glycosylated; expresses both type 1 and type 2 core glycans.
Heavily N-glycosylated; expresses primarily high mannose and complex bisecting type N-linked glycans.
May be phosphorylated. Phosphorylation of the intracellular C-terminal domain may induce proteolytic cleavage and the liberation of the extracellular domain into the extracellular space.
May contain numerous disulfide bridges. Association of several molecules of the secreted form may occur through interchain disulfide bridges providing an extraordinarily large gel-like matrix in the extracellular space or in the lumen of secretory ducts. -
细胞定位Cell membrane. Secreted > extracellular space. May be liberated into the extracellular space following the phosphorylation of the intracellular C-terminus which induces the proteolytic cleavage and liberation of the extracellular domain.
- Information by UniProt
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数据库链接
- Entrez Gene: 94025 Human
- Omim: 606154 Human
- SwissProt: Q8WXI7 Human
- Unigene: 432676 Human
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别名
- CA 125 antibody
- CA-125 antibody
- CA125 antibody
see all
图片
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![Flow Cytometry - Anti-MUC16 antibody [OC125] (ab693)](https://www.abcam.cn/ps/products/0/ab693/Images/MUC16-Primary-antibodies-ab693-1.jpg)
Flow Cytometry - Anti-MUC16 antibody [OC125] (ab693)Overlay histogram showing HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained with ab693 (red line). The cells were fixed with 80% methanol (5 minutes) and then permeabilized with 0.1% PBS-Tween for 20 minutes. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab693, 1 µg/1x106 cells) for 30 minutes at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 minutes at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2 µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 minutes)/permeabilized with 0.1% PBS-Tween for 20 minutes used under the same conditions.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC16 antibody [OC125] (ab693)](https://www.abcam.cn/ps/products/0/ab693/Images/ab693-284421-ab6931333.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC16 antibody [OC125] (ab693)Paraffin embedded human ovarian cancer tissue stained for MUC16 with ab693 (1/50 dilution) in immunohistochemical analysis.
数据表及文件
文献
This product has been referenced in:
- Kline JB et al. Tumor antigen CA125 suppresses antibody-dependent cellular cytotoxicity (ADCC) via direct antibody binding and suppressed Fc-? receptor engagement. Oncotarget 8:52045-52060 (2017). Flow Cyt ; Human . Read more (PubMed: 28881712) »
- Kim BR et al. SOX2 and PI3K Cooperate to Induce and Stabilize a Squamous-Committed Stem Cell Injury State during Lung Squamous Cell Carcinoma Pathogenesis. PLoS Biol 14:e1002581 (2016). ICC/IF ; Human . Read more (PubMed: 27880766) »
