Anti-MUC16抗体[OC125] (ab693)

Submit a review Q&A (6)References (12)
Flow Cytometry - Anti-MUC16 antibody [OC125] (ab693)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC16 antibody [OC125] (ab693)

Key features and details

  • Mouse monoclonal [OC125] to MUC16
  • Suitable for: Flow Cyt, IHC-P
  • Reacts with: Human
  • Isotype: IgG1

概述

  • 产品名称

    Anti-MUC16抗体[OC125]
    参阅全部 MUC16 一抗

  • 描述

    小鼠单克隆抗体[OC125] to MUC16

  • 宿主

    Mouse

  • 特异性

    Studies have show that this antibody reacts with approximately 80% of epithelial ovarian neoplasms of serous, endometrioid, clear cell and undifferentiated types. No reactivity has been shown for mucinous ovarian tumors or in germ cell or hematopoietic tumors. It reacts with both normal tissues and neoplasms of fallopian tube, endometrium, endocervix and mesothelioma. It does not react with colon cancer. Normal tissues such as breast, liver, skin, kidney and spleen are negative.

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    See all applications and species data

  • 免疫原

    Partially purified human mucin fraction from a pool of tissues from patients with epithelial ovarian cancer.

  • 表位

    This antibody recognizes an epitope on a molecule called Cancer Antigen 125 (CA125).

  • 阳性对照

    • Flow cytometry: HeLa cells. IHC-P: Ovarian cancer tissue.

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

性能

应用

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab693 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

应用 Species
Flow Cyt
Human
IHC-P
Human
应用 Ab评论 说明
Flow Cyt
Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. ABC Method.
说明
Flow Cyt
Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. ABC Method.

靶标

  • 功能

    Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces.

  • 组织特异性

    Expressed in corneal and conjunctival epithelia (at protein level). Overexpressed in ovarian carcinomas and ovarian low malignant potential (LMP) tumors as compared to the expression in normal ovarian tissue and ovarian adenomas.

  • 序列相似性

    Contains 2 ANK repeats.
    Contains 56 SEA domains.

  • 结构域

    Composed of three domains, a Ser-, Thr-rich N-terminal domain, a repeated domain containing more than 60 partially conserved tandem repeats of 156 amino acids each (AAs 12061-21862) and a C-terminal transmembrane contain domain with a short cytoplasmic tail.

  • 翻译后修饰

    Heavily O-glycosylated; expresses both type 1 and type 2 core glycans.
    Heavily N-glycosylated; expresses primarily high mannose and complex bisecting type N-linked glycans.
    May be phosphorylated. Phosphorylation of the intracellular C-terminal domain may induce proteolytic cleavage and the liberation of the extracellular domain into the extracellular space.
    May contain numerous disulfide bridges. Association of several molecules of the secreted form may occur through interchain disulfide bridges providing an extraordinarily large gel-like matrix in the extracellular space or in the lumen of secretory ducts.

  • 细胞定位

    Cell membrane. Secreted > extracellular space. May be liberated into the extracellular space following the phosphorylation of the intracellular C-terminus which induces the proteolytic cleavage and liberation of the extracellular domain.
  • Information by UniProt

  • 数据库链接

    • 别名

      • CA 125 antibody
      • CA-125 antibody
      • CA125 antibody
      • CA125 ovarian cancer antigen antibody
      • Cancer antigen 125 antibody
      • FLJ14303 antibody
      • MUC 16 antibody
      • MUC-16 antibody
      • MUC16 antibody
      • MUC16_HUMAN antibody
      • Mucin 16 antibody
      • Mucin 16 cell surface associated antibody
      • Mucin-16 antibody
      • Mucin16 antibody
      • Ovarian cancer related tumor marker CA125 antibody
      • Ovarian cancer-related tumor marker CA125 antibody
      • Ovarian carcinoma antigen CA125 antibody
      see all

    图片

    • Flow Cytometry - Anti-MUC16 antibody [OC125] (ab693)
      Flow Cytometry - Anti-MUC16 antibody [OC125] (ab693)

      Overlay histogram showing HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained with ab693 (red line). The cells were fixed with 80% methanol (5 minutes) and then permeabilized with 0.1% PBS-Tween for 20 minutes. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab693, 1 µg/1x106 cells) for 30 minutes at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 minutes at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2 µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 minutes)/permeabilized with 0.1% PBS-Tween for 20 minutes used under the same conditions.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC16 antibody [OC125] (ab693)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC16 antibody [OC125] (ab693)

      Paraffin embedded human ovarian cancer tissue stained for MUC16 with ab693 (1/50 dilution) in immunohistochemical analysis.

    文献 (12)

    发表研究结果有使用 ab693？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab693 被引用在 12 文献中.

    • Pantshwa JM  et al. Chemotherapeutic Efficacy of Implantable Antineoplastic-Treatment Protocols in an Optimal Mouse Model for Human Ovarian Carcinoma Cell Targeting. Int J Mol Sci 19:N/A (2018). PubMed: 30287783
    • Kline JB  et al. Tumor antigen CA125 suppresses antibody-dependent cellular cytotoxicity (ADCC) via direct antibody binding and suppressed Fc-? receptor engagement. Oncotarget 8:52045-52060 (2017). Flow Cyt ; Human . PubMed: 28881712
    • Kim BR  et al. SOX2 and PI3K Cooperate to Induce and Stabilize a Squamous-Committed Stem Cell Injury State during Lung Squamous Cell Carcinoma Pathogenesis. PLoS Biol 14:e1002581 (2016). ICC/IF ; Human . PubMed: 27880766
    • Zeng WJ  et al. Quantum dot-based multiplexed imaging in malignant ascites: a new model for malignant ascites classification. Int J Nanomedicine 10:1759-68 (2015). IHC-P ; Human . PubMed: 25784803
    • Zhang J  et al. Megakaryocytic potentiating factor and mature mesothelin stimulate the growth of a lung cancer cell line in the peritoneal cavity of mice. PLoS One 9:e104388 (2014). Flow Cyt ; Mouse . PubMed: 25118887
    View all Publications for this product

    客户评价及客户问答

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    1-6 of 6 Abreviews or Q&A

    Question

    I am inquiring about the antigen peptide about the anti-MUC16 antibody [OC125] (ab693). I am now studying the structure and function of MUC16. If I could know in which region this antibody could recognize the protein, it will be a great help for my study. UCLA HEALTH SCIENCES IMPORTANT WARNING: This email (and any attachments) is only intended for the use of the person or entity to which it is addressed, and may contain information that is privileged and confidential. You, the recipient, are obligated to maintain it in a safe, secure and confidential manner. Unauthorized redisclosure or failure to maintain confidentiality may subject you to federal and state penalties. If you are not the intended recipient, please immediately notify us by return email, and delete this message from your computer.

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    Answer

    Thank you for your interest in the MUC16 antibody, clone OC125. The papers from 1981 and 1983 that are listed in the references section of the ab693 datasheet describe production and characterization of the antibody. The antigen was not a peptide; it was a cell line, OVCA433, established from a patient with cystadenocarcinoma. (http://www.ncbi.nlm.nih.gov/pmc/articles/PMC370929/?page=2) The location of the epitope that the antibody recognizes has not, to our knowledge, been determined.

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    Question

    Could you give me information about concentration of MAB, α-hu OC125 (α-hu Muc16). A F/P-ratio would be likewise available?

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    Answer

    The IgG concentration of the lot you have is 25.5 ug/mL. The antibody is not conjugated, so there is no F/P ratio.

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    Question


    Hello-

    I had a question about your anti-MUC16 antibody OC125 (cat#ab693). On your website it says it is sold as 500 uL, but I could not find a concentration listed. Could you please let me know what concentration this product is provided at? Thank you very much for your time!

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    Answer

    Thank you for your reply. The concentration for the current lot is 50.5 ug/mL. Please let me know if you have any further questions.

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    Question

    Hello-
    I had a question about your anti-MUC16 antibody OC125 (cat#ab693). On your website it says it is sold as 500 uL, but I could not find a concentration listed. Could you please let me know what concentration this product is provided at? Thank you very much for your time!

    Read More
    Answer

    Thank you for contacting us. The concentration is batch dependent. Have you already purchased the product? If so I can provide the concentration if you can send the lot number. Thank you!

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    Question

    I would like to test ab693 in IP.

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    Answer

    I am very pleased to hear you would like to accept our offer and test ab693 in IP. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for IP and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.  

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    Question

    He needs this produkt to storting with FACS and he has to do life staining.

    He need this cells to grow

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    Answer

    Thank you for your reply and for clarifying the questions.

    I can confirm that both these antibodies (ab693 and ab134093) are tested and covered by our 6 month guarantee for flow cytometry.

    However, I am sorry they have not been tried and tested for FACS cell sorting where live cells are required, so regrettably we would not be able to guarantee how well this will work. Both contain low concentration of preservative which may affect the cell viability.

    I hope this will be helpful. If you have any further questions, please do not hesitate to contact me.

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