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Tags & Cell Markers Cell Type Markers Tumor Associated
使用敲除细胞株进行验证重组RabMAb

重组Anti-MUC1抗体[EPR1023] (ab109185)

  • Datasheet
  • SDS
Reviews (1)Q&A (5)References (18)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC1 antibody [EPR1023] (ab109185)
  • Western blot - Anti-MUC1 antibody [EPR1023] (ab109185)
  • Western blot - Anti-MUC1 antibody [EPR1023] (ab109185)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC1 antibody [EPR1023] (ab109185)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC1 antibody [EPR1023] (ab109185)
  • Immunocytochemistry/ Immunofluorescence - Anti-MUC1 antibody [EPR1023] (ab109185)
  • Western blot - Anti-MUC1 antibody [EPR1023] (ab109185)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC1 antibody [EPR1023] (ab109185)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC1 antibody [EPR1023] (ab109185)
  • Immunoprecipitation - Anti-MUC1 antibody [EPR1023] (ab109185)
  • Flow Cytometry - Anti-MUC1 antibody [EPR1023] (ab109185)
  • Western blot - Anti-MUC1 antibody [EPR1023] (ab109185)
  • Flow Cytometry - Anti-MUC1 antibody [EPR1023] (ab109185)
  • OI-RD Scanning - Anti-MUC1 antibody [EPR1023] (ab109185)
  • Anti-MUC1 antibody [EPR1023] (ab109185)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR1023] to MUC1
  • Suitable for: ICC/IF, WB, IP, IHC-P, Flow Cyt
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

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概述

  • 产品名称

    Anti-MUC1抗体[EPR1023]
    参阅全部 MUC1 一抗
  • 描述

    兔单克隆抗体[EPR1023] to MUC1
  • 宿主

    Rabbit
  • 特异性

    ab109185 detects the 17 kDa carboxy-terminal subunit (subunit beta). Depending on the N-glycosylation extent, the size of this subunit is estimated to be between 17 kDa (without N-glycosylation) and 23-25 kDa
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Rat
    Human
    IP
    Human
    WB
    Mouse
    Human
    See all applications and species data
  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • T47-D cell lysate, colon cancer lysate, Human breast carcinoma tissue, Human normal breast tissue.
  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • 解离常数(KD)

    KD = 8.90 x 10 -12 M
    Learn more about KD
  • 存储溶液

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 59% PBS, 0.05% BSA
  • Concentration information loading...
  • 纯度

    Protein A purified
  • 克隆

    单克隆
  • 克隆编号

    EPR1023
  • 同种型

    IgG
  • 研究领域

    • Tags & Cell Markers
    • Cell Type Markers
    • Tumor Associated
    • Signal Transduction
    • Cytoskeleton / ECM
    • Extracellular Matrix
    • ECM Proteins
    • MUC
    • Cancer
    • Invasion/microenvironment
    • ECM
    • Extracellular matrix
    • MUC
    • Cancer
    • Tumor immunology
    • Tumor-associated antigens

相关产品

  • Alternative Versions

    • Anti-MUC1 antibody [EPR1023] - Low endotoxin, Azide free (ab194978)
    • HRP Anti-MUC1 antibody [EPR1023] (ab195936)
    • Alexa Fluor® 488 Anti-MUC1 antibody [EPR1023] (ab196443)
    • PE Anti-MUC1 antibody [EPR1023] (ab211592)
    • Anti-MUC1 antibody [EPR1023] - BSA and Azide free (ab271879)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • T-47D whole cell lysate (ab14899)
  • Recombinant Protein

    • Recombinant Human MUC1 protein (ab80082)

应用

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab109185 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

应用 Species
Flow Cyt
Human
ICC/IF
Human
IHC-P
Rat
Human
IP
Human
WB
Mouse
Human
应用 Ab评论 说明
ICC/IF
1/1000.

For unpurified use at 1/100 - 1/250.

WB
1/1000 - 1/5000. Predicted molecular weight: 17 kDa.
IP
1/20.

For unpurified use at 1/10 - 1/100.

IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

Flow Cyt
1/30.

For unpurified use at 1/100 - 1/1000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

说明
ICC/IF
1/1000.

For unpurified use at 1/100 - 1/250.

WB
1/1000 - 1/5000. Predicted molecular weight: 17 kDa.
IP
1/20.

For unpurified use at 1/10 - 1/100.

IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

Flow Cyt
1/30.

For unpurified use at 1/100 - 1/1000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

靶标

  • 功能

    The alpha subunit has cell adhesive properties. Can act both as an adhesion and an anti-adhesion protein. May provide a protective layer on epithelial cells against bacterial and enzyme attack.
    The beta subunit contains a C-terminal domain which is involved in cell signaling, through phosphorylations and protein-protein interactions. Modulates signaling in ERK, SRC and NF-kappa-B pathways. In activated T-cells, influences directly or indirectly the Ras/MAPK pathway. Promotes tumor progression. Regulates TP53-mediated transcription and determines cell fate in the genotoxic stress response. Binds, together with KLF4, the PE21 promoter element of TP53 and represses TP53 activity.
  • 组织特异性

    Expressed on the apical surface of epithelial cells, especially of airway passages, breast and uterus. Also expressed in activated and unactivated T-cells. Overexpressed in epithelial tumors, such as breast or ovarian cancer and also in non-epithelial tumor cells. Isoform Y is expressed in tumor cells only.
  • 疾病相关

    MUC1/CA 15-3 is used as a serological clinical marker of breast cancer to monitor response to breast cancer treatment and disease recurrence (PubMed:20816948). Decreased levels over time may be indicative of a positive response to treatment. Conversely, increased levels may indicate disease progression. At an early stage disease, only 21% of patients exhibit high MUC1/CA 15-3 levels, that is why CA 15-3 is not a useful screening test. Most antibodies target the highly immunodominant core peptide domain of 20 amino acid (APDTRPAPGSTAPPAHGVTS) tandem repeats. Some antibodies recognize glycosylated epitopes.
    Medullary cystic kidney disease 1
  • 序列相似性

    Contains 1 SEA domain.
  • 发展阶段

    During fetal development, expressed at low levels in the colonic epithelium from 13 weeks of gestation.
  • 翻译后修饰

    Highly glycosylated (N- and O-linked carbohydrates and sialic acid). O-glycosylated to a varying degree on serine and threonine residues within each tandem repeat, ranging from mono- to penta-glycosylation. The average density ranges from about 50% in human milk to over 90% in T47D breast cancer cells. Further sialylation occurs during recycling. Membrane-shed glycoproteins from kidney and breast cancer cells have preferentially sialyated core 1 structures, while secreted forms from the same tissues display mainly core 2 structures. The O-glycosylated content is overlapping in both these tissues with terminal fucose and galactose, 2- and 3-linked galactose, 3- and 3,6-linked GalNAc-ol and 4-linked GlcNAc predominating. Differentially O-glycosylated in breast carcinomas with 3,4-linked GlcNAc. N-glycosylation consists of high-mannose, acidic complex-type and hybrid glycans in the secreted form MUC1/SEC, and neutral complex-type in the transmembrane form, MUC1/TM.
    Proteolytic cleavage in the SEA domain occurs in the endoplasmic reticulum by an autoproteolytic mechanism and requires the full-length SEA domain as well as requiring a Ser, Thr or Cys residue at the P + 1 site. Cleavage at this site also occurs on isoform MUC1/X but not on isoform MUC1/Y. Ectodomain shedding is mediated by ADAM17.
    Dual palmitoylation on cysteine residues in the CQC motif is required for recycling from endosomes back to the plasma membrane.
    Phosphorylated on tyrosines and serine residues in the C-terminal. Phosphorylation on tyrosines in the C-terminal increases the nuclear location of MUC1 and beta-catenin. Phosphorylation by PKC delta induces binding of MUC1 to beta-catenin/CTNNB1 and thus decreases the formation of the beta-catenin/E-cadherin complex. Src-mediated phosphorylation inhibits interaction with GSK3B. Src- and EGFR-mediated phosphorylation on Tyr-1229 increases binding to beta-catenin/CTNNB1. GSK3B-mediated phosphorylation on Ser-1227 decreases this interaction but restores the formation of the beta-cadherin/E-cadherin complex. On T-cell receptor activation, phosphorylated by LCK. PDGFR-mediated phosphorylation increases nuclear colocalization of MUC1CT and CTNNB1.
    The N-terminal sequence has been shown to begin at position 24 or 28.
  • 细胞定位

    Secreted; Cell membrane. Cytoplasm. Nucleus. On EGF and PDGFRB stimulation, transported to the nucleus through interaction with CTNNB1, a process which is stimulated by phosphorylation. On HRG stimulation, colocalizes with JUP/gamma-catenin at the nucleus and Apical cell membrane. Exclusively located in the apical domain of the plasma membrane of highly polarized epithelial cells. After endocytosis, internalized and recycled to the cell membrane. Located to microvilli and to the tips of long filopodial protusions.
  • Target information above from: UniProt accession P15941 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 4582 Human
    • Entrez Gene: 17829 Mouse
    • Entrez Gene: 24571 Rat
    • Omim: 158340 Human
    • SwissProt: P15941 Human
    • SwissProt: Q02496 Mouse
    • Unigene: 89603 Human
    • Unigene: 16193 Mouse
    • 别名

      • ADMCKD antibody
      • ADMCKD1 antibody
      • Breast carcinoma associated antigen DF3 antibody
      • Breast carcinoma-associated antigen DF3 antibody
      • CA 15-3 antibody
      • CA15 3 antibody
      • CA15 3 antigen antibody
      • CA15-3 antibody
      • CA15.3 antibody
      • Cancer antigen 15-3 antibody
      • Carcinoma associated mucin antibody
      • Carcinoma-associated mucin antibody
      • CD 227 antibody
      • CD227 antibody
      • DF3 antigen antibody
      • EMA antibody
      • Episialin antibody
      • Epithelial Membrane Antigen antibody
      • H23 antigen antibody
      • H23AG antibody
      • KL 6 antibody
      • KL-6 antibody
      • KL6 antibody
      • Krebs von den Lungen-6 antibody
      • MAM 6 antibody
      • MAM6 antibody
      • MCD antibody
      • MCKD antibody
      • MCKD1 antibody
      • Medullary cystic kidney disease 1 (autosomal dominant) antibody
      • Medullary cystic kidney disease, autosomal dominant antibody
      • MUC 1 antibody
      • MUC-1 antibody
      • MUC-1/SEC antibody
      • MUC-1/X antibody
      • MUC1 antibody
      • MUC1-alpha antibody
      • MUC1-beta antibody
      • MUC1-CT antibody
      • MUC1-NT antibody
      • MUC1/ZD antibody
      • MUC1_HUMAN antibody
      • Mucin 1 antibody
      • Mucin 1 cell surface associated antibody
      • Mucin 1 transmembrane antibody
      • Mucin 1, cell surface associated antibody
      • Mucin-1 subunit beta antibody
      • Peanut reactive urinary mucin antibody
      • Peanut-reactive urinary mucin antibody
      • PEM antibody
      • PEMT antibody
      • Polymorphic epithelial mucin antibody
      • PUM antibody
      • Tumor associated epithelial membrane antigen antibody
      • Tumor associated epithelial mucin antibody
      • Tumor associated mucin antibody
      • Tumor-associated epithelial membrane antigen antibody
      • Tumor-associated mucin antibody
      see all

    图片

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC1 antibody [EPR1023] (ab109185)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC1 antibody [EPR1023] (ab109185)

      Immunohistochemical staining of paraffin embedded human endometrium with purified ab109185 at a working dilution of 1 in 500. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

    • Western blot - Anti-MUC1 antibody [EPR1023] (ab109185)
      Western blot - Anti-MUC1 antibody [EPR1023] (ab109185)
      All lanes : Anti-MUC1 antibody [EPR1023] (ab109185) at 1/1000 dilution

      Lane 1 : Wild-type HeLa whole cell lysate
      Lane 2 : MUC1 knockout HeLa whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Predicted band size: 17 kDa
      Observed band size: 17-24 kDa why is the actual band size different from the predicted?



      Lanes 1 - 2: Merged signal (red and green). Green - ab109185 observed at 24 kDa. Red - loading control, ab9484, observed at 37 kDa.

      ab109185 was shown to specifically react with MUC1 in wild-type HeLa cells as signal was lost in MUC1 knockout cells. Wild-type and MUC1 knockout samples were subjected to SDS-PAGE. Ab109185 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    • Western blot - Anti-MUC1 antibody [EPR1023] (ab109185)
      Western blot - Anti-MUC1 antibody [EPR1023] (ab109185)
      Anti-MUC1 antibody [EPR1023] (ab109185) at 1/1000 dilution (Purified antibody) + Colon cancer at 10 µg

      Secondary
      Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

      Predicted band size: 17 kDa
      Observed band size: 24 kDa why is the actual band size different from the predicted?



      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC1 antibody [EPR1023] (ab109185)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC1 antibody [EPR1023] (ab109185)

      Immunohistochemical staining of paraffin embedded mouse lung with purified ab109185 at a working dilution of 1 in 500. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC1 antibody [EPR1023] (ab109185)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC1 antibody [EPR1023] (ab109185)

      Immunohistochemical staining of paraffin embedded rat kidney with purified ab109185 at a working dilution of 1 in 500. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

    • Immunocytochemistry/ Immunofluorescence - Anti-MUC1 antibody [EPR1023] (ab109185)
      Immunocytochemistry/ Immunofluorescence - Anti-MUC1 antibody [EPR1023] (ab109185)

      Immunofluorescence staining of A431 cells with purified ab109185 at a working dilution of 1 in 1000, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 555 goat anti rabbit, used at a dilution of 1 in 400. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative control is shown in bottom right hand panel - for the negative control, purified ab109185 was used at a dilution of 1/200 followed by an Alexa Fluor® 555 goat anti-mouse antibody at a dilution of 1/500.

    • Western blot - Anti-MUC1 antibody [EPR1023] (ab109185)
      Western blot - Anti-MUC1 antibody [EPR1023] (ab109185)
      Anti-MUC1 antibody [EPR1023] (ab109185) at 1/5000 dilution (Purified) + T47D cell lysate at 10 µg

      Secondary
      Secondary ab: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

      Predicted band size: 17 kDa
      Observed band size: 18-25 kDa why is the actual band size different from the predicted?



      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC1 antibody [EPR1023] (ab109185)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC1 antibody [EPR1023] (ab109185)

      ab109185 (unpurified) showing positive staining in Breast ductal infiltrating carcinoma tissue.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC1 antibody [EPR1023] (ab109185)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC1 antibody [EPR1023] (ab109185)

      ab109185 (unpurified) showing positive staining in Normal stomach tissue.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunoprecipitation - Anti-MUC1 antibody [EPR1023] (ab109185)
      Immunoprecipitation - Anti-MUC1 antibody [EPR1023] (ab109185)

      ab109185 (purified) at 1/20 immunoprecipitating MUC1 in T47-D (Lane 1). Lane 2 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).

      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.

    • Flow Cytometry - Anti-MUC1 antibody [EPR1023] (ab109185)
      Flow Cytometry - Anti-MUC1 antibody [EPR1023] (ab109185)

      Overlay histogram showing MCF7 cells fixed in 2% PFA and stained with purified ab109185 at a dilution of 1 in 30 (pink line). The secondary antibody used was FITC goat anti-rabbit at a dilution of 1 in 150. Rabbit monoclonal IgG was used as an isotype control.

    • Western blot - Anti-MUC1 antibody [EPR1023] (ab109185)
      Western blot - Anti-MUC1 antibody [EPR1023] (ab109185)
      All lanes : Anti-MUC1 antibody [EPR1023] (ab109185) at 1/1000 dilution (Unpurified)

      Lane 1 : T47-D cell lysate
      Lane 2 : Human colon cancer lysate

      Lysates/proteins at 10 µg per lane.

      Performed under reducing conditions.

      Predicted band size: 17 kDa
      Additional bands at: 17 kDa (possible cleavage fragment)

    • Flow Cytometry - Anti-MUC1 antibody [EPR1023] (ab109185)
      Flow Cytometry - Anti-MUC1 antibody [EPR1023] (ab109185)

      Overlay histogram showing MCF7 cells stained with unpurified ab109185 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab109185, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in MCF7 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

    • OI-RD Scanning - Anti-MUC1 antibody [EPR1023] (ab109185)
      OI-RD Scanning - Anti-MUC1 antibody [EPR1023] (ab109185)

      Equilibrium disassociation constant (KD) of unpurified ab109185.
      Learn more about KD

      Click here to learn more about KD

    • Anti-MUC1 antibody [EPR1023] (ab109185)
      Anti-MUC1 antibody [EPR1023] (ab109185)

    实验方案

    • Flow cytometry protocols
    • Immunoprecipitation protocols
    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    数据表及文件

    • SDS download

    • Datasheet download

      Download

    文献 (18)

    发表研究结果有使用 ab109185?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab109185 被引用在 18 文献中.

    • Fan Z  et al. Smartphone Biosensor System with Multi-Testing Unit Based on Localized Surface Plasmon Resonance Integrated with Microfluidics Chip. Sensors (Basel) 20:N/A (2020). PubMed: 31941128
    • Li XY  et al. The long noncoding RNA MIR210HG promotes tumor metastasis by acting as a ceRNA of miR-1226-3p to regulate mucin-1c expression in invasive breast cancer. Aging (Albany NY) 11:5646-5665 (2019). PubMed: 31399552
    • Huang R  et al. The construction and analysis of tumor-infiltrating immune cell and ceRNA networks in recurrent soft tissue sarcoma. Aging (Albany NY) 11:10116-10143 (2019). PubMed: 31739284
    • Wu G  et al. A Mucin1 C-terminal Subunit-directed Monoclonal Antibody Targets Overexpressed Mucin1 in Breast Cancer. Theranostics 8:78-91 (2018). PubMed: 29290794
    • Xiang S  et al. Crosstalk of NF-?B/P65 and LncRNA HOTAIR-Mediated Repression of MUC1 Expression Contribute to Synergistic Inhibition of Castration-Resistant Prostate Cancer by Polyphyllin 1-Enzalutamide Combination Treatment. Cell Physiol Biochem 47:759-773 (2018). PubMed: 29807357
    View all Publications for this product

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    1-6 of 6 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-MUC1 antibody [EPR1023]

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (sebocytes)
    Permeabilization
    Yes - PBS 0,5% tween
    Specification
    sebocytes
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
    Fixative
    Formaldehyde
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    Abcam user community

    Verified customer

    提交于 Dec 27 2019

    Question

    Which MUC1 isoforms will be detected by ab109185?

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    Abcam community

    Verified customer

    Asked on Sep 11 2014

    Answer

    The MUC1 antibody ab109185 should be capable of detecting all isoforms of human MUC1 that are listed in the UniProt entry for accession P15941, http://www.uniprot.org/uniprot/P15941, with the exception of isoforms 5, M6, ZD, and J13, which are missing the sequence of amino acids that the antibody is raised against. However, detection of specific isoforms has not been confirmed by experiment.

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    Tom Ruyle

    Abcam Scientific Support

    回复于 Sep 11 2014

    Question

    In the picture below are bands about 22 kDa, but in description there is - Predicted band size : 122 kDa. We think that this is some mistake. Customer thinks also that she should expect bond 122 kDa . Will this protein be glycolysed? On the other side 22kDa is too little for MUC1 protein.

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    Abcam community

    Verified customer

    Asked on Jun 05 2013

    Answer


    MUC1 has at least 10 isoforms with molecular weights around either 400 kDa or 20 kDa. ab109185 is designed to recognize a 25 kDa C-term cleaved fragment which is why the observed band is at ˜ 25 KDa.

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    Abcam Scientific Support

    回复于 Jun 05 2013

    Question

    Inquiry: Hi, Could you let me know which "residues from the C-terminus of Human MUC1" were used to form the synthetic peptide immunogen for this antibody? (ab109185) Thank you!

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    Abcam community

    Verified customer

    Asked on Apr 24 2012

    Answer

    Thank you for your enquiry and your interest in our products.

    The immunogen used to raise this antibody was a synthetic peptide, corresponding to residues from the C-terminus of Human MUC1. The exact sequence is commercially sensitive information.

    The epitope has not been mapped yet; as soon as we have further information we will update our online datasheet accordingly.

    If you need any further assistance in the future, please do not hesitate to contact me.

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    Abcam Scientific Support

    回复于 Apr 24 2012

    Question

    thank you for the answer. i see that in your catalog you have 4 MUC1 protein. Can you suggest me witch one i 've to select for the antibody that you advise me?? Could you advise me the antigen for the Ab28081?





    best regards,

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    Abcam community

    Verified customer

    Asked on Jan 27 2012

    Answer

    Thank you for your inquiry.

    Unfortunately, we do not offer a MUC1 fulllength protein. The MUC1 protein (ab80082) is only a recombinant fragment and all others are peptides. I am sorry for the inconvenience but I cannot recommend any of these as positive control for your experiment.

    The immunogen for ab28081 washuman Urinary MUC-1 mucin.I am happy to confirm that the epitopethis clone ([C595 (NCRC48)])recognizes was mapped to the peptide epitope ARG-PRO-ALA-PRO within the protein core of the mucin. The epitope sequence RPAP is a repeated motif that occurs about 40 times along the length of the protein (between residues 126 to 965).

    I am sorry that I could not answer all your questions on this occasion and hope this information was helpful nevertheless.

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    Abcam Scientific Support

    回复于 Jan 27 2012

    Question

    Dear AbCam,
    i would like to perform an ELISA sandwich assay for the determination of MUC1 antigen using this two antibodies:
    1) ab28081
    2) ab109185
    i would like to know if it possible to use these two antibodies together; if these antibodies are not yet tested,
    is possible to have a discount or a free sample if i decide to purchase?

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    Abcam community

    Verified customer

    Asked on Jan 26 2012

    Answer

    Thank you for your inquiry.

    I can confirm that these two antibodies were not tested in sandwich ELISA yet. Unfortunately, we cannot give any discount. But there would be the possiblity to take part in our testing discount programm and to recieve a free antibody.

    But I cannot recommend it in this case because we do not even know if ab109185 work in ELISA at all and we do have antibodies that are tested and guaranteed for sandwich ELISA in our catalogue:

    ab10119 (Anti-MUC1 antibody [M3A106] (HRP)) can be be paired for ELISA with Mouse monoclonal [M10H6] to MUC1 (ab10117) and Mouse monoclonal [M4H2] to MUC1 (ab10120) and Mouse monoclonal [M8C9] to MUC1 (ab10123).

    Please follow these links to the respective datasheets:

    Click here (or use the following: https://www.abcam.com/MUC1-antibody-M3A106-HRP-ab10119.html).

    Click here (or use the following: https://www.abcam.com/MUC1-antibody-M10H6-ab10117.html).

    Click here (or use the following: https://www.abcam.com/MUC1-antibody-M4H2-ab10120.html).

    Click here (or use the following: https://www.abcam.com/MUC1-antibody-M8C9-ab10123.html).

    I hope this information is helpful. Please do not hesiate to conatc me with any further qestions.

    Read More

    Abcam Scientific Support

    回复于 Jan 26 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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