Question (74229) | Anti-mTOR (phospho S2448) antibody [EPR426(2)] (ab109268)

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Question

Thank you for your help. And I want to ask a question about how 0.1% PBS-TWEEN permeabilize cells? Could TWEEN do it? If I add TWEEN of other company to 1XPBS buffer to a final volume fraction of 0.1%, could this solution also permealize cells?

Answer

Tween-20 is often used at 0.2%-0.5% as a membrane permeabilizing solution. At 0.1% in PBS, Tween-20 can help reduce background but does not have much effect for permeabilizing. Tween-20 may be found at many life science vendors.

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