为了使您在Abcam官网的浏览体验更顺畅,请使用最新版本的浏览器比如 Google Chrome

我们使用cookies,使我们网站尽可能对您有帮助。

我们的Cookie政策介绍了您如何能够取消cookie使用。

如果您不改变cookie设置,我们认为您愿意接受cookie。

继续 继续

致电 +86 21 2070 0500 或 联系我们

  • 我的账户
  • 退出
登录 或 注册

欢迎光临

登录 或

您还没有帐户?

注册
我的购物篮
快速订购
Abcam homepage

  • 研究用途的产品
    按产品类型
    一抗
    二抗
    免疫测定试剂盒与试剂
    细胞与组织成像工具
    细胞与生化测定
    蛋白质与肽
    按产品类型
    蛋白质组学工具
    激动剂、活化剂、拮抗剂与抑制剂
    细胞株和裂解液
    多重miRNA检测
    多因子分析试剂盒
    按研究领域
    癌症
    心血管
    细胞生物学
    表观遗传学
    代谢
    发育生物学
    按研究领域
    免疫学
    微生物学
    神经科学
    信号转导
    干细胞
  • 诊断和治疗的解决方案
    定制方案及合作关系

    定制抗体开发及商务合作助力您的诊断和治疗探索。

    与我们一起开创定制方案

    与我们成为伙伴

  • 技术支持
    支持中心

    获取研究建议和支持

    查看支持中心

    Protocols

    一步一步完成您的实验

    查看Protocols

  • 活动通知
    • 会议年历
    • 肿瘤研究
    • 心血管研究
    • 染色质及细胞核信号转导研究
    • 免疫学
    • 神经生物学
    • 干细胞研究
    • 商品展览
    • 实验技术视频
    查看最新活动

    活动说明、摘要提交、演讲者、注册方式及更多信息

    查看全球活动安排

  • 通路
    细胞信号转导通路

    查看所有通路

    查看交叉通路

在新冠疫情期间支持我们的客户和员工–了解更多

Cell Biology Cell Cycle Cell Cycle Inhibitors Other

Anti-mTOR (phospho S2448)抗体(ab1093)

  • Datasheet
  • SDS
Submit a review Q&A (1)References (17)

Product price, shipping and contact information

Currently unavailable

抱歉,我们目前无法显示此内容。

请联系我们以完成下单,或者之后再尝试访问。

 

正在加载规格和价格。。。

 

送货和订单信息

Abpromise

产品质量保证,客户专家支持,

点击获取更多

Western blot - Anti-mTOR (phospho S2448) antibody (ab1093)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mTOR (phospho S2448) antibody (ab1093)

Key features and details

  • Rabbit polyclonal to mTOR (phospho S2448)
  • Suitable for: IHC-P, WB
  • Reacts with: Human
  • Isotype: IgG

你可能会感兴趣

蛋白
Product image
Recombinant Human mTOR protein (ab114179)
二抗
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
一抗
Product image
Anti-HLA-DR antibody [EPR3692] - Low endotoxin, Azide free (ab215985)

查看更多相关产品

概述

  • 产品名称

    Anti-mTOR (phospho S2448)抗体
    参阅全部 mTOR 一抗
  • 描述

    兔多克隆抗体to mTOR (phospho S2448)
  • 宿主

    Rabbit
  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data
  • 免疫原

    Synthetic peptide: KRSRTRTDpSYSAGQSVEI, corresponding to C terminal amino acids 2440-2463 of Human mTOR.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 阳性对照

    • WB: IGF-1 treated serum starved 293T whole cell lysate. IHC-P: Human kidney tissue, proximal convoluted tubules.

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液

    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Cell Biology
    • Cell Cycle
    • Cell Cycle Inhibitors
    • Other
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Damage & Repair
    • DNA Damage Response
    • DNA Damage Recognition
    • Cancer
    • Cell cycle
    • Cell cycle inhibitors
    • Other
    • Cardiovascular
    • Heart
    • Cardiogenesis
    • Transcription factors/regulators
    • Kits/ Lysates/ Other
    • Kits
    • ELISA Kits
    • ELISA Kits
    • Growth factors and hormones ELISA kits
    • Metabolism
    • Types of disease
    • Obesity

相关产品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human mTOR protein (ab114179)

应用

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab1093 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

应用 Species
IHC-P
Human
WB
Human
应用 Ab评论 说明
IHC-P
Use a concentration of 5 µg/ml.
WB
1/1000. Detects a band of approximately 250 kDa (predicted molecular weight: 288 kDa).
说明
IHC-P
Use a concentration of 5 µg/ml.
WB
1/1000. Detects a band of approximately 250 kDa (predicted molecular weight: 288 kDa).

靶标

  • 功能

    Kinase subunit of both mTORC1 and mTORC2, which regulates cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino-acids. Growth factor-stimulated mTORC1 activation involves AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino-acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-421', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Phosphorylates MAF1 leading to attenuation of its RNA polymerase III-repressive function. mTORC2 is also activated by growth. factors, but seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'.
  • 组织特异性

    Expressed in numerous tissues, with highest levels in testis.
  • 序列相似性

    Belongs to the PI3/PI4-kinase family.
    Contains 1 FAT domain.
    Contains 1 FATC domain.
    Contains 7 HEAT repeats.
    Contains 1 PI3K/PI4K domain.
  • 翻译后修饰

    Autophosphorylated; when part of mTORC1 or mTORC2.
  • 细胞定位

    Endoplasmic reticulum membrane. Golgi apparatus membrane. Mitochondrion outer membrane. Lysosome. Cytoplasm. Nucleus > PML body. Shuttles between cytoplasm and nucleus. Accumulates in the nucleus in response to hypoxia (By similarity). Targeting to lysosomes depends on amino acid availability and RRAGA and RRAGB.
  • Target information above from: UniProt accession P42345 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 2475 Human
    • Omim: 601231 Human
    • SwissProt: P42345 Human
    • Unigene: 338207 Human
    • 别名

      • dJ576K7.1 (FK506 binding protein 12 rapamycin associated protein 1) antibody
      • FK506 binding protein 12 rapamycin associated protein 1 antibody
      • FK506 binding protein 12 rapamycin associated protein 2 antibody
      • FK506 binding protein 12 rapamycin complex associated protein 1 antibody
      • FK506-binding protein 12-rapamycin complex-associated protein 1 antibody
      • FKBP rapamycin associated protein antibody
      • FKBP12 rapamycin complex associated protein antibody
      • FKBP12-rapamycin complex-associated protein 1 antibody
      • FKBP12-rapamycin complex-associated protein antibody
      • FLJ44809 antibody
      • FRAP antibody
      • FRAP1 antibody
      • FRAP2 antibody
      • Mammalian target of rapamycin antibody
      • Mechanistic target of rapamycin antibody
      • mTOR antibody
      • MTOR_HUMAN antibody
      • OTTHUMP00000001983 antibody
      • RAFT1 antibody
      • Rapamycin and FKBP12 target 1 antibody
      • Rapamycin associated protein FRAP2 antibody
      • Rapamycin target protein 1 antibody
      • Rapamycin target protein antibody
      • RAPT1 antibody
      • Serine/threonine-protein kinase mTOR antibody
      see all

    图片

    • Western blot - Anti-mTOR (phospho S2448) antibody (ab1093)
      Western blot - Anti-mTOR (phospho S2448) antibody (ab1093)
      All lanes : Anti-mTOR (phospho S2448) antibody (ab1093) at 1/1000 dilution

      Lane 1 : 24hr serum starved 293T whole cell lysate
      Lane 2 : 24hr serum starved 293T whole cell lysate, treated with IGF-1 (100 ng/ml) for 20 mins prior to harvest

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : HRP Goat-anti-Rabbit IgG at 1/2000 dilution

      Predicted band size: 288 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mTOR (phospho S2448) antibody (ab1093)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mTOR (phospho S2448) antibody (ab1093)

      Formalin-fixed, paraffin-embedded human kidney tissue, (proximal convulted tubules), labeling mTOR using ab1093 at 5 µg/ml (red). Counterstain is hematoxylin.

    实验方案

    • Western blot protocols
    • Immunohistochemistry protocols

    Click here to view the general protocols

    数据表及文件

    • SDS download

    • Datasheet download

      Download

    文献 (17)

    发表研究结果有使用 ab1093?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab1093 被引用在 17 文献中.

    • Teng CF  et al. Chemopreventive Effect of Phytosomal Curcumin on Hepatitis B Virus-Related Hepatocellular Carcinoma in A Transgenic Mouse Model. Sci Rep 9:10338 (2019). PubMed: 31316146
    • Liu Y  et al. Alisol B 23-acetate inhibits the viability and induces apoptosis of non-small cell lung cancer cells via PI3K/AKT/mTOR signal pathway. Mol Med Rep 20:1187-1195 (2019). PubMed: 31173235
    • Feng CW  et al. Therapeutic Effect of Modulating TREM-1 via Anti-inflammation and Autophagy in Parkinson's Disease. Front Neurosci 13:769 (2019). PubMed: 31440123
    • Tao J  et al. MiR-216a accelerates proliferation and fibrogenesis via targeting PTEN and SMAD7 in human cardiac fibroblasts. Cardiovasc Diagn Ther 9:535-544 (2019). PubMed: 32038943
    • Ortega MA  et al. Implication of the PI3K/Akt/mTOR Pathway in the Process of Incompetent Valves in Patients with Chronic Venous Insufficiency and the Relationship with Aging. Oxid Med Cell Longev 2018:1495170 (2018). PubMed: 30057669
    View all Publications for this product

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    Question

    BATCH NUMBER 125112 ORDER NUMBER 94471 DESCRIPTION OF THE PROBLEM No signal at all SAMPLE WCL isolated from cultured HepG2 , HCT116, DLD1, SUV480 and HT29 cells PRIMARY ANTIBODY Dilution: 1:1000 Dilution buffer: TBS-Tween 20(0.1%) with 5% BSA incubation: overnight at 4 degrees Waching step: wash with TBS-Tween 20(0.1%) , 5min x 3 DETECTION METHOD ECL plus system POSITIVE AND NEGATIVE CONTROLS USED No, the customer didn't use both positive and negative controls. ANTIBODY STORAGE CONDITIONS It was stored at -20 degrees. SAMPLE PREPARATION Lysis buffer: CelLytic-M from SIGMA (cat# C2978) Protease inhibitors: protease inhibitor coacktai tablets from Roche (cat# 11697 498001) Loading buffer: TrisGlycine-SDS sample buffer (2x) from Invitrogen Heating sample: for 5 minutes at 95 degrees AMOUNT OF PROTEIN LOADED approximately 20ug each ELECTROPHORESIS/GEL CONDITIONS 4-12% Tris-Glycine Gel (1.0mm thick) from Invitrogen TRANSFER AND BLOCKING CONDITIONS Blocking buffer: TBS-Tween 20 (0.1%) Blocking agent: 5% non-fat dry milk incubation : for 1 hour at room temp SECONDARY ANTIBODY 2nd Ab: Anti rabbit IgG-HRP conjugated Dilution: 1:10000 Incubation: for 1 hour at room temp Wash step: TBS-Tween 20(0.1%), 5min x 3 HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 1 ADDITIONAL NOTES Would you please let me know how we should advise her about this matter?

    Read More

    Abcam community

    Verified customer

    Asked on Aug 24 2005

    Answer

    I'm sorry to hear one of your customers is experiencing a problem with ab1093. We have not tested this antibody in cultured HepG2 , HCT116, DLD1, SUV480 and HT29 cells and I think the customer's problem is due to the fact that these cells contain low levels of the phosphorylated protein, undetectable by WB with ab1093. For example, a paper by Paajarvi et al (FASEB J. 2005 Mar;19(3):476-8. Epub 2004 Dec 29.) shows that HepG2 cells (even serum starved) have very low levels of basal phosphoS448 mTOR and need to be stimulated with pravastin 2uM and insulin 1ugl/ml (1hr) to have a significant level of the phosphorylated protein. Therefore I strongly suggest that the customer runs the recommended positive control of serum starved 293T cell lysate (preferably nuclear lysate) and to also do a nuclear extraction step to concentrate the protein in her samples. Please ask her to try a 1:500 dilution of the antibody and make sure the secondary antibody is not damaged. I also noticed the customer blocked with two blocking agents (BSA and milk), this can cause problems too so ask her to do a 1hr 5%BSA block and use only TBST to dilute the primary and secondary antibodies (milk contains casein which is a phospho protein so causes interference with phospho-specific antibodies). Please let me know if you need any further assistance,

    Read More

    Abcam Scientific Support

    回复于 Aug 26 2005

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    通过邮件收到学术资源和促销信息 注册
    研究类别(A-Z)
    • 肿瘤研究
    • 心血管研究
    • 细胞生物学
    • 发育生物学
    • 染色质及细胞核信号转导研究
    • 免疫学
    • 代谢
    • 微生物学
    • 神经生物学
    • 信号转导研究
    • 干细胞研究
    产品种类(A-Z)
    • 一抗
    • 二抗
    • 生化试剂
    • 亚类对照
    • 流式细胞仪多色选择器
    • 试剂盒
    • 内参对照
    • 细胞裂解液
    • 多肽
    • 蛋白酶
    • 组织切片
    • 蛋白标签及细胞标记物
    • 相关试剂及工具
    帮助与支持
    • 技术支持
    • 技术FAQ
    • 实验步骤与建议
    • 购买FAQ
    • RabMAb 产品
    • 培训课程
    • 按靶标浏览
    企业
    • 企业资讯
    • 投资者关系
    • 公司新闻
    • 招贤纳士
    • 关于我们
    活动通知
    • 商品展览
    • 会议
    全球网站
    • abcam.com
    • abcam.co.jp
    QR code for Weechat

    微信扫码 关注我们

    沪ICP备14046373号-1

    沪公网安备 31011502007413号

    • 销售条款
    • 网站使用条款
    • Cookie政策
    • 隐私权政策
    • 法律
    © 1998-2021 艾博抗(上海)贸易有限公司版权所有