Application
Western blot
Sample
Human Cell lysate - whole cell (Keratinocyte)
Loading amount
15 µg
Specification
Keratinocyte
Gel Running Conditions
Reduced Denaturing (10% gel)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Other product details
Dilution
1/1000
Incubation time
20 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBS
Secondary antibody
Name
Non-Abcam antibody was used: GE (Amersham) ECL Rabbit IgG, HRP-Linked F(ab')2 F
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
West Pico (Pierce)
Exposure
20 minute(s) and 0 second(s)
Bands
Specific: >175 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Prof. Paul Townsend
Verified customer
提交于 Jun 26 2008