Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Other product details
2 hour(s) and 0 minute(s) · Temperature: 22°C
Non-Abcam antibody was used: Jackson ImmunoResearch anti-rabbit Host species: Donkey Clonality: Polyclonal Conjugation: Horse Radish Peroxidase
15 hour(s), 0 minute(s) and 0 second(s)
30 ug HeLa cell lysates
The anti-rabbit secondary antibody from Jackson ImmunoResearch we used for this experiment was shown to work well for other rabbit antibodies. The exposure time from 1 min to overnight (15 hours) did not give any band. Whether this is a vial-specific problem remains to be determined.
We are sorry to hear that ab32028 did not work well in your Western Blotting experiment. At this time, we have not received any recent complaints on this product and we are unsure about the cause of the problem. As you indicated, the problem could be vial specific. We would be happy to offer you another vial/lot to test. Please keep us updated with your progress.