Application
Western blot
Sample
Mouse Tissue lysate - whole (Cerebellum)
Loading amount
30 µg
Specification
Cerebellum
Gel Running Conditions
Reduced Denaturing (4-20 % gel)
Blocking step
Starting Block T20 (PBS) as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C
Other product details
Dilution
1/2000
Incubation time
22 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Starting Block T20 (PBS)
Secondary antibody
Name
Non-Abcam antibody was used: Goat ECL anti-rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
Chemiluminescence, ECL
Exposure
1 minute(s) and 30 second(s)
Bands
Specific: 280 kDa Non-specific: 150 kDa
Positive control
i used this mouse cerebellum as a positive control
Negative control
i did not have a negative control
Additional data
Additional Notes
I previously used this antibody on rat penis and detected a strong band around 150kDa. I ordered the blocking peptide to make sure this was the correct band and it turned out the actual band was detected at around 250-280kDa. I ran another gel using this mouse cerebellum as a positive control and noticed a nice strong band at the molecular weight of interest which is about 250-280 kDa.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Miss. Gwen Lagoda
Verified customer
提交于 Jul 15 2008