Key features and details
- Rabbit polyclonal to MTG16
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
存储溶液Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
Concentration information loading...
纯度Immunogen affinity purified
Our Abpromise guarantee covers the use of ab129444 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 71 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
功能Functions as a transcriptional repressor. Regulates the proliferation and the differentiation of erythroid progenitors by repressing the expression of TAL1 target genes. Plays a role in granulocyte differentiation.
Isoform 2 functions as an A-kinase-anchoring protein.
组织特异性Widely expressed with higher expression in heart, pancreas, skeletal muscle, spleen, thymus and peripheral blood leukocytes. Expressed in hematopoietic cells (at protein level).
疾病相关Note=A chromosomal aberration involving CBFA2T3 is found in therapy-related myeloid malignancies. Translocation t(16;21)(q24;q22) that forms a RUNX1-CBFA2T3 fusion protein.
序列相似性Belongs to the CBFA2T family.
Contains 1 MYND-type zinc finger.
Contains 1 TAFH (NHR1) domain.
细胞定位Nucleus > nucleolus. The RUNX1-CBFA2T3 fusion protein localizes to the nucleoplasm and Nucleus > nucleoplasm. Golgi apparatus membrane.
- Information by UniProt
- Cbfa2t3 antibody
- Ccore binding factor runt domain alpha subunit 2 translocated to 3 antibody
- ETO2 antibody
All lanes : Anti-MTG16 antibody (ab129444) at 0.1 µg/ml
Lane 1 : Jurkat whole cell lysate at 50 µg
Lane 2 : Jurkat whole cell lysate at 15 µg
Lane 3 : 293T whole cell lysate at 50 µg
Lane 4 : HeLa whole cell lysate at 50 µg
Predicted band size: 71 kDa
Detection of MTG16 by Western Blot of Immunprecipitate.
Lane 1: ab129444 at 1µg/ml staining MTG16 in HeLa whole cell lysate immunoprecipitated using ab129444 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Lane 2: Control IgG
Detection: Chemiluminescence with exposure time of 10 seconds.