参阅全部 MT-ND5 一抗
经测试应用适用于: WB, ELISA, IHC-Pmore details
Synthetic peptide between 550~579 amino acids selected from the C terminal region of Human MT-ND5 conjugated to KLH.
- WB: CEM cell lysate. IHC-P: Human normal cerebral cortex.
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
存储溶液Preservative: 0.09% Sodium azide
Concentration information loading...
纯度Immunogen affinity purified
纯化说明Purified through a protein A column, followed by peptide affinity purification.
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Integration of energy
Our Abpromise guarantee covers the use of ab92624 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/100 - 1/1000. Predicted molecular weight: 67 kDa.|
|IHC-P||1/25 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
功能Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
疾病相关Defects in MT-ND5 are a cause of Leber hereditary optic neuropathy (LHON) [MIM:535000]. LHON is a maternally inherited disease resulting in acute or subacute loss of central vision, due to optic nerve dysfunction. Cardiac conduction defects and neurological defects have also been described in some patients. LHON results from primary mitochondrial DNA mutations affecting the respiratory chain complexes.
Defects in MT-ND5 are a cause of Leigh syndrome (LS) [MIM:256000]. LS is a severe neurological disorder characterized by bilaterally symmetrical necrotic lesions in subcortical brain regions.
Defects in MT-ND5 are a cause of mitochondrial complex I deficiency (MT-C1D) [MIM:252010]. A disorder of the mitochondrial respiratory chain that causes a wide range of clinical disorders, from lethal neonatal disease to adult-onset neurodegenerative disorders. Phenotypes include macrocephaly with progressive leukodystrophy, non-specific encephalopathy, cardiomyopathy, myopathy, liver disease, Leigh syndrome, Leber hereditary optic neuropathy, and some forms of Parkinson disease.
Defects in MT-ND5 are a cause of mitochondrial encephalomyopathy with lactic acidosis and stroke-like episodes syndrome (MELAS) [MIM:540000]. MELAS is a genetically heterogenious disorder, characterized by episodic vomiting, seizures, and recurrent cerebral insults resembling strokes and causing hemiparesis, hemianopsia, or cortical blindness.
序列相似性Belongs to the complex I subunit 5 family.
细胞定位Mitochondrion inner membrane.
- Information by UniProt
- Complex I, subunit ND5 antibody
- EC 188.8.131.52 antibody
- Mitochondrially encoded NADH dehydrogenase 5 antibody
IHC image of MT-ND5 staining in Human normal cerebral cortex formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab92624, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Anti-MT-ND5 antibody (ab92624) at 1/1000 dilution + CEM whole cell lysate at 35 µg
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution
Predicted band size: 67 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes : Anti-MT-ND5 antibody (ab92624) at 1/100 dilution
All lanes : Human Cerebral Cortex
Lysates/proteins at 20 µg per lane.
All lanes : Donkey polyclonal to rabbit IgG conjugated to IRDye 800CW at 1/500 dilution
Performed under reducing conditions.
Predicted band size: 67 kDa
Exposure time: 5 minutes
Detection method: Odyssey system
This product has been referenced in:
- Lin H et al. CO2-sensitive tRNA modification associated with human mitochondrial disease. Nat Commun 9:1875 (2018). Read more (PubMed: 29760464) »
- Zhou M et al. A hypertension-associated mitochondrial DNA mutation introduces an m1G37 modification into tRNAMet, altering its structure and function. J Biol Chem 293:1425-1438 (2018). Read more (PubMed: 29222331) »