重组Anti-MT-ND1抗体[EPR13466(2)] (ab181848)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13466(2)] to MT-ND1
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-MT-ND1抗体[EPR13466(2)]
参阅全部 MT-ND1 一抗 -
描述
兔单克隆抗体[EPR13466(2)] to MT-ND1 -
宿主
Rabbit -
特异性
This antibody is unsuitable for detecting cell line samples.
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经测试应用
适用于: WBmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Human fetal heart and fetal muscle lysates.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.21% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR13466(2) -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab181848于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB | (3) |
1/1000 - 1/20000. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).
This antibody is unsuitable for detecting cell line samples. |
说明 |
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WB
1/1000 - 1/20000. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa). This antibody is unsuitable for detecting cell line samples. |
靶标
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功能
Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. -
疾病相关
Defects in MT-ND1 are a cause of Leber hereditary optic neuropathy (LHON) [MIM:535000]. LHON is a maternally inherited disease resulting in acute or subacute loss of central vision, due to optic nerve dysfunction. Cardiac conduction defects and neurological defects have also been described in some patients. LHON results from primary mitochondrial DNA mutations affecting the respiratory chain complexes.
Defects in MT-ND1 are a cause of mitochondrial encephalomyopathy with lactic acidosis and stroke-like episodes syndrome (MELAS) [MIM:540000]. MELAS is a genetically heterogenious disorder, characterized by episodic vomiting, seizures, and recurrent cerebral insults resembling strokes and causing hemiparesis, hemianopsia, or cortical blindness.
Defects in MT-ND1 may be associated with susceptibility to Alzheimer disease mitochondrial (AD-MT) [MIM:502500]. Alzheimer disease is a neurodegenerative disorder characterized by progressive dementia, loss of cognitive abilities, and deposition of fibrillar amyloid proteins as intraneuronal neurofibrillary tangles, extracellular amyloid plaques and vascular amyloid deposits. The major constituent of these plaques is the neurotoxic amyloid-beta-APP 40-42 peptide (s), derived proteolytically from the transmembrane precursor protein APP by sequential secretase processing. The cytotoxic C-terminal fragments (CTFs) and the caspase-cleaved products such as C31 derived from APP, are also implicated in neuronal death.
Defects in MT-ND1 may be associated with non-insulin-dependent diabetes mellitus (NIDDM). -
序列相似性
Belongs to the complex I subunit 1 family. -
细胞定位
Mitochondrion inner membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 4535 Human
- Entrez Gene: 17716 Mouse
- Entrez Gene: 26193 Rat
- Omim: 516000 Human
- SwissProt: P03886 Human
- SwissProt: P03888 Mouse
- SwissProt: P03889 Rat
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别名
- Complex I, subunit ND1 antibody
- Mitochondrially encoded NADH dehydrogenase 1 antibody
- MT-ND1 antibody
see all
图片
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All lanes : Anti-MT-ND1 antibody [EPR13466(2)] (ab181848) at 1/5000 dilution (purified)
Lane 1 : Human heart lysates
Lane 2 : Mouse heart lysates
Lane 3 : Rat heart lysates
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 36 kDaBlocking and diluting buffer: 5% NFDM/TBST
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All lanes : Anti-MT-ND1 antibody [EPR13466(2)] (ab181848) at 1/20000 dilution (unpurified)
Lane 1 : Human fetal heart lysate
Lane 2 : Human fetal muscle lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution
Predicted band size: 36 kDa
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (25)
ab181848 被引用在 25 文献中.
- Suliman HB et al. Nuclear respiratory factor-1 negatively regulates TGF-β1 and attenuates pulmonary fibrosis. iScience 25:103535 (2022). PubMed: 34977500
- Zhu J et al. Clinical Relevance and Tumor Growth Suppression of Mitochondrial ROS Regulators along NADH:Ubiquinone Oxidoreductase Subunit B3 in Thyroid Cancer. Oxid Med Cell Longev 2022:8038857 (2022). PubMed: 35087620
- Emtenani S et al. Macrophage mitochondrial bioenergetics and tissue invasion are boosted by an Atossa-Porthos axis in Drosophila. EMBO J 41:e109049 (2022). PubMed: 35319107
- Chen X et al. Neuropathy-associated Fars2 deficiency affects neuronal development and potentiates neuronal apoptosis by impairing mitochondrial function. Cell Biosci 12:103 (2022). PubMed: 35794642
- Bertola N et al. Mutated FANCA Gene Role in the Modulation of Energy Metabolism and Mitochondrial Dynamics in Head and Neck Squamous Cell Carcinoma. Cells 11:N/A (2022). PubMed: 35954197