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Epigenetics and Nuclear Signaling Transcription Other factors
使用敲除细胞株进行验证重组RabMAb

重组Anti-mSin3A抗体[EPR6780] (ab129087)

  • Datasheet
  • SDS
Submit a review Submit a question References (5)

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Western blot - Anti-mSin3A antibody [EPR6780] (ab129087)
  • Immunocytochemistry/ Immunofluorescence - Anti-mSin3A antibody [EPR6780] (ab129087)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mSin3A antibody [EPR6780] (ab129087)
  • Flow Cytometry - Anti-mSin3A antibody [EPR6780] (ab129087)
  • Western blot - Anti-mSin3A antibody [EPR6780] (ab129087)
  • OI-RD Scanning - Anti-mSin3A antibody [EPR6780] (ab129087)
  • Anti-mSin3A antibody [EPR6780] (ab129087)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6780] to mSin3A
  • Suitable for: WB, IHC-P, Flow Cyt, ICC/IF
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

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概述

  • 产品名称

    Anti-mSin3A抗体[EPR6780]
    参阅全部 mSin3A 一抗
  • 描述

    兔单克隆抗体[EPR6780] to mSin3A
  • 宿主

    Rabbit
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data
  • 免疫原

    Synthetic peptide corresponding to residues on the C-terminus in Human mSin3A (UniProt ID: Q96ST3).

  • 阳性对照

    • HeLa, K562, 293T, PC12, RAW 264.7, and Mouse lung lysates, Human colon tissue
  • 常规说明

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • 解离常数(KD)

    KD = 1.19 x 10 -10 M
    Learn more about KD
  • 存储溶液

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • 纯度

    Tissue culture supernatant
  • 克隆

    单克隆
  • 克隆编号

    EPR6780
  • 同种型

    IgG
  • 研究领域

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Other factors
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Co-factors
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Metabolism of lipids and lipoproteins
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Lipid metabolism
    • Metabolism
    • Pathways and Processes
    • Cofactors, Vitamins / minerals
    • Co-factors

相关产品

  • Alternative Versions

    • Anti-mSin3A antibody [EPR6780] - BSA and Azide free (ab248291)
  • Compatible Secondaries

    • Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • HeLa nuclear extract lysate (ab14655)
    • K-562 nuclear extract lysate (ab14851)
    • PC-12 nuclear extract lysate (ab14884)

应用

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab129087 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

应用 Species
Flow Cyt
Human
ICC/IF
Human
IHC-P
Human
WB
Human
应用 Ab评论 说明
WB
1/1000 - 1/10000. Detects a band of approximately 150 kDa (predicted molecular weight: 145 kDa).
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt
Use at an assay dependent concentration.
ICC/IF
1/100 - 1/250.
说明
WB
1/1000 - 1/10000. Detects a band of approximately 150 kDa (predicted molecular weight: 145 kDa).
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt
Use at an assay dependent concentration.
ICC/IF
1/100 - 1/250.
  • 应用说明
    Is unsuitable for IP.
  • 靶标

    • 功能

      Acts as a transcriptional repressor. Interacts with MXI1 to repress MYC responsive genes and antagonize MYC oncogenic activities. Also interacts with MAD-MAX heterodimers by binding to MAD. The heterodimer then represses transcription by tethering SIN3A to DNA. Acts as a corepressor for REST.
    • 序列相似性

      Contains 3 PAH (paired amphipathic helix) domains.
    • 翻译后修饰

      SUMO1 sumoylated by TOPORS. Probably desumoylated by SENP2.
    • 细胞定位

      Nucleus. Nucleus > nucleolus. Recruited to the nucleolus by SAP30L.
    • Target information above from: UniProt accession Q96ST3 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • 数据库链接

      • Entrez Gene: 25942 Human
      • Entrez Gene: 20466 Mouse
      • Entrez Gene: 363067 Rat
      • Omim: 607776 Human
      • SwissProt: Q96ST3 Human
      • SwissProt: Q60520 Mouse
      • Unigene: 513039 Human
      • Unigene: 15755 Mouse
      • Unigene: 63749 Rat
      see all
    • 别名

      • AW553200 antibody
      • DKFZP434K2235 antibody
      • FLJ90319 antibody
      • Histone deacetylase complex subunit Sin 3a antibody
      • Histone deacetylase complex subunit Sin3a antibody
      • KIAA0700 antibody
      • Kiaa4126 antibody
      • mKIAA4126 antibody
      • Paired amphipathic helix protein Sin 3a antibody
      • Paired amphipathic helix protein Sin3a antibody
      • Sin 3a antibody
      • SIN3 homolog A antibody
      • SIN3 homolog A transcription regulator (yeast) antibody
      • SIN3 homolog A transcription regulator antibody
      • SIN3 transcription regulator homolog A antibody
      • Sin3a antibody
      • SIN3A protein antibody
      • SIN3A_HUMAN antibody
      • Transcriptional co repressor Sin 3A antibody
      • Transcriptional co repressor Sin3A antibody
      • Transcriptional corepressor Sin3a antibody
      • Transcriptional regulator SIN3A antibody
      see all

    图片

    • Western blot - Anti-mSin3A antibody [EPR6780] (ab129087)
      Western blot - Anti-mSin3A antibody [EPR6780] (ab129087)

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: mSin3A knockout HAP1 cell lysate (20 µg)
      Lane 3: HeLa cell lysate (20 µg)
      Lane 4: Jurkat cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab129087 observed at 150 kDa. Red - loading control, ab8245, observed at 37 kDa.
      ab129087 was shown to recognize mSin3A when mSin3A knockout samples were used, along with additional cross-reactive bands. Wild-type and mSinA knockout samples were subjected to SDS-PAGE. ab129087 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

    • Immunocytochemistry/ Immunofluorescence - Anti-mSin3A antibody [EPR6780] (ab129087)
      Immunocytochemistry/ Immunofluorescence - Anti-mSin3A antibody [EPR6780] (ab129087)

      Immunocytochemistry/Immunofluorescence analysis of MCF7 (Human breast adenocarcinoma cell line) labeling mSin3A with Purified ab129087 at 1/500 dilution (5 µg/ml). Cells were fixed with 4% PFA and permeabilized with 0.1% tritonX-100. ab150077 Goat anti rabbit IgG(Alexa Fluor® 488) at 1/1000 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI. PBS was used instead of the primary antibody as the negative control.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mSin3A antibody [EPR6780] (ab129087)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mSin3A antibody [EPR6780] (ab129087)

      ab129087, at a dilution of 1/50, staining mSin3A in paraffin-embedded Human colon tissue by Immunohistochemistry.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Flow Cytometry - Anti-mSin3A antibody [EPR6780] (ab129087)
      Flow Cytometry - Anti-mSin3A antibody [EPR6780] (ab129087)

      Flow Cytometry analysis of MCF-7 (human breast carcinoma) cells labeling mSin3A with purified ab129087 at 1/250 dilution(10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

    • Western blot - Anti-mSin3A antibody [EPR6780] (ab129087)
      Western blot - Anti-mSin3A antibody [EPR6780] (ab129087)
      All lanes : Anti-mSin3A antibody [EPR6780] (ab129087) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : K562 cell lysate
      Lane 3 : 293T cell lysate
      Lane 4 : PC12 cell lysate
      Lane 5 : RAW 264.7 cell lysate
      Lane 6 : Mouse lung lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 145 kDa

    • OI-RD Scanning - Anti-mSin3A antibody [EPR6780] (ab129087)
      OI-RD Scanning - Anti-mSin3A antibody [EPR6780] (ab129087)
      Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD
    • Anti-mSin3A antibody [EPR6780] (ab129087)
      Anti-mSin3A antibody [EPR6780] (ab129087)

    实验方案

    • Flow cytometry protocols
    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    数据表及文件

    • SDS download

    • Datasheet download

      Download

    文献 (5)

    发表研究结果有使用 ab129087?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab129087 被引用在 5 文献中.

    • Kalin JH  et al. Targeting the CoREST complex with dual histone deacetylase and demethylase inhibitors. Nat Commun 9:53 (2018). WB . PubMed: 29302039
    • Mehta SL  et al. Long Noncoding RNA FosDT Promotes Ischemic Brain Injury by Interacting with REST-Associated Chromatin-Modifying Proteins. J Neurosci 35:16443-9 (2015). PubMed: 26674869
    • Jamaladdin S  et al. Histone deacetylase (HDAC) 1 and 2 are essential for accurate cell division and the pluripotency of embryonic stem cells. Proc Natl Acad Sci U S A 111:9840-5 (2014). Mouse . PubMed: 24958871
    • Cho S  et al. AGO2 and SETDB1 cooperate in promoter-targeted transcriptional silencing of the androgen receptor gene. Nucleic Acids Res N/A:N/A (2014). PubMed: 25183519
    • O'Neill DJ  et al. SETD6 controls the expression of estrogen-responsive genes and proliferation of breast carcinoma cells. Epigenetics 9:942-50 (2014). PubMed: 24751716

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