Application
Western blot
Sample
Mouse Tissue lysate - whole (Liver homogenate)
Loading amount
25 µg
Specification
Liver homogenate
Gel Running Conditions
Reduced Denaturing (7.5%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Dilution
1/250
Incubation time
2 hour(s) and 30 minute(s) · Temperature: 25°C · Diluent: TBS+tween
Secondary antibody
Name
Dilution
1/25000
Detection
Detection method
ECL+
Exposure
6 minute(s) and 30 second(s)
Bands
Specific: 200 kDa
Additional data
Additional Notes
Each lane represents an individual WT mouse sample
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Sep 17 2009