Key features and details
- Mouse monoclonal [M2 III-6] to MRP2
- Suitable for: IHC-P, IHC-Fr, WB, ICC/IF
- Knockout validated
- Reacts with: Human
- Isotype: IgG2a
参阅全部 MRP2 一抗
描述小鼠单克隆抗体[M2 III-6] to MRP2
特异性This antibody detects MRP 2. It does not cross-react with human MDR 1, MRP 1, MRP 3 or MRP 5 gene products.
经测试应用适用于: IHC-P, IHC-Fr, WB, ICC/IFmore details
Fusion protein corresponding to Human MRP2 aa 1339-1541 (C terminal).
表位This antibody reacts with an internal epitope of MRP 2.
We received mixed feedback about rat species so we are removing this from tested species list for now. The immunogen is 84% homologous to human protein so theoretically it should cross react however considering the latest customer feedback we no longer guarantee this species.
存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Constituent: 0.7% BSA
Concentration information loading...
Our Abpromise guarantee covers the use of ab3373 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/200. See Abreview. Abreview indicates that several methods of antigen retreival are suitable for this antibody, but heat mediated Ag retrieval in citrate buffer gave optimal results.|
Acetone fixation of frozen sample is recommended.
|WB||1/20 - 1/50. Predicted molecular weight: 174 kDa.
See Scheffer GL et al. 2000.
The samples MUST NOT be boiled before running the gel as multi-pass transmembrane proteins, like MRP2, aggregate severely at high temperatures, which increases as a direct function of temperature. We recommend heating the samples at 60-70C for 15-20 minutes or at 36C for 20 minutes as mentioned in PLoS Pathog 9:e1003244 (2013).
|ICC/IF||1/20 - 1/50.
Acetone fixed cytospin preparations.
功能Mediates hepatobiliary excretion of numerous organic anions. May function as a cellular cisplatin transporter.
组织特异性Found on the apical membrane of polarized cells in liver, kidney and intestine. The highest expression is found in liver.
疾病相关Defects in ABCC2 are the cause of Dubin-Johnson syndrome (DJS) [MIM:237500]. DJS is an autosomal recessive disorder characterized by conjugated hyperbilirubinemia, an increase in the urinary excretion of coproporphyrin isomer I, deposition of melanin-like pigment in hepatocytes, and prolonged retention of sulfobromophthalein, but otherwise normal liver function.
序列相似性Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily.
Contains 2 ABC transmembrane type-1 domains.
Contains 2 ABC transporter domains.
- Information by UniProt
- ABC30 antibody
- abcC2 antibody
- ATP binding cassette sub family C (CFTR/MRP) member 2 antibody
All lanes : Anti-MRP2 antibody [M2 III-6] (ab3373) at 1/20 dilution
Lane 1 : Wild-type A549 whole cell lysate
Lane 2 : ABCC2 knockout A549 whole cell lysate
Lane 3 : HepG2 whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 174 kDa
Lanes 1 - 3: Merged signal (red and green). Green - ab3373 observed at 210 kDa. Red - loading control, ab52866, observed at 50 kDa.
ab3373 was shown to recognize ABCC2 in wild-type A549 cells as signal was lost at the expected MW in ABCC2 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and ABCC2 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% Milk. Ab3373 and ab52866 (Rabbit anti-alpha Tubulin loading control) were incubated overnight at 4°C at 1/20 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
Confocal micrographs comparing the presence and localization of hepatic markers within a 2D cell culture of human hepatocytes. Staining β-Catenin with ab32572 at 1/100 (Purple) and MRP2 with ab3373 at 1/100 (Yellow).
Cells were fixed for 30 minutes at 4°C in 4% paraformaldehyde (PFA) and washed 3 times with DPBS. Cells were blocked for 1 hour with DPBS containing 1% donkey serum, 1% Triton X-100. Cells were incubated for 1 hour at room temperature with the primary antibodies diluted in the blocking solution. Cells were washed three times with PBS for 30 minutes each. Cells were incubated for 1 hour at room temperature with appropriate secondary antibodies diluted in the blocking solution. Cells were then washed three times with PBS for 30 minutes each and then imaged using an LSM700 laser scanning confocal microscope.
Immunohistochemical analysis of Human liver tissue, staining MRP2 with ab3373 at 1/50 dilution.
Anti-MRP2 antibody [M2 III-6] (ab3373) at 1/500 dilution + WIF-B cells, the hepatoma-derived hybrid cell line at 30 µg with 5% milk in PBST
HRP conjugated Donkey anti-Mouse at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 174 kDa
Observed band size: 130-250 kDa why is the actual band size different from the predicted?
Exposure time: 20 minutes
ab3373 被引用在 57 文献中.
- Xiao Y et al. Quercetin and kaempferol increase the intestinal absorption of isorhamnetin coexisting in Elaeagnus rhamnoides (L.) A. Nelson (Elaeagnaceae) extracts via regulating multidrug resistance-associated protein 2. Phytomedicine 53:154-162 (2019). PubMed: 30668394
- Myint K et al. Identification of MRP2 as a targetable factor limiting oxaliplatin accumulation and response in gastrointestinal cancer. Sci Rep 9:2245 (2019). PubMed: 30783141
- Costantini D et al. Simulated microgravity promotes the formation of tridimensional cultures and stimulates pluripotency and a glycolytic metabolism in human hepatic and biliary tree stem/progenitor cells. Sci Rep 9:5559 (2019). PubMed: 30944365
- Haran JP et al. Alzheimer's Disease Microbiome Is Associated with Dysregulation of the Anti-Inflammatory P-Glycoprotein Pathway. mBio 10:N/A (2019). PubMed: 31064831
- Cervenkova L et al. Protein expression of ABCC2 and SLC22A3 associates with prognosis of pancreatic adenocarcinoma. Sci Rep 9:19782 (2019). PubMed: 31874997