重组Anti-MRP2抗体[EPR10998] (ab172630)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10998] to MRP2
- Suitable for: WB, ICC/IF, Flow Cyt (Intra)
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-MRP2抗体[EPR10998]
参阅全部 MRP2 一抗 -
描述
兔单克隆抗体[EPR10998] to MRP2 -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IF, Flow Cyt (Intra)more details
不适用于: IHC-P or IP -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Human fetal liver, HepG2 and A549 lysates. ICC/IF: HepG2 cells and A549 cells. Flow Cyt (intra): Permeabilized A549 cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR10998 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Conjugation kits
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Isotype control
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KO cell lines
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KO cell lysates
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab172630于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 174 kDa.
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ICC/IF |
1/100.
For unpurified use at 1/250 - 1/500. |
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Flow Cyt (Intra) |
1/70.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. For unpurified use at 1/100 - 1/500. |
说明 |
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WB
1/1000 - 1/10000. Predicted molecular weight: 174 kDa. |
ICC/IF
1/100. For unpurified use at 1/250 - 1/500. |
Flow Cyt (Intra)
1/70. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. For unpurified use at 1/100 - 1/500. |
靶标
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功能
Mediates hepatobiliary excretion of numerous organic anions. May function as a cellular cisplatin transporter. -
组织特异性
Found on the apical membrane of polarized cells in liver, kidney and intestine. The highest expression is found in liver. -
疾病相关
Defects in ABCC2 are the cause of Dubin-Johnson syndrome (DJS) [MIM:237500]. DJS is an autosomal recessive disorder characterized by conjugated hyperbilirubinemia, an increase in the urinary excretion of coproporphyrin isomer I, deposition of melanin-like pigment in hepatocytes, and prolonged retention of sulfobromophthalein, but otherwise normal liver function. -
序列相似性
Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily.
Contains 2 ABC transmembrane type-1 domains.
Contains 2 ABC transporter domains. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 1244 Human
- Omim: 601107 Human
- SwissProt: Q92887 Human
- Unigene: 368243 Human
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别名
- ABC30 antibody
- abcC2 antibody
- ATP binding cassette sub family C (CFTR/MRP) member 2 antibody
see all
图片
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All lanes : Anti-MRP2 antibody [EPR10998] (ab172630) at 1/1000 dilution
Lane 1 : Wild-type A549 (Human lung carcinoma cell line) whole cell lysate
Lane 2 : ABCC2 knockout A549 (Human lung carcinoma cell line) whole cell lysate
Lane 3 : Hep G2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 4 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 174 kDaLanes 1 - 4: Merged signal (red and green). Green - ab172630 observed at 210 kDa. Red - loading control, ab130007, observed at 130 kDa.
ab172630 was shown to recognize MRP2 in wild-type A549 cells as signal was lost at the expected MW in ABCC2 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and ABCC2 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% Milk. Ab172630 and ab130007 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/1000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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Anti-MRP2 antibody [EPR10998] (ab172630) at 1/2000 dilution (Purified) + HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 174 kDa
Observed band size: >190 kDa why is the actual band size different from the predicted?The multiple bands are consistent with what has been described in PMID: 18832477
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Immunocytochemistry/ Immunofluorescence analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling MRP2 with purified ab172630 at 1:100 dilution (7.1 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with None. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
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Intracellular Flow Cytometry analysis of A549 (Human lung carcinoma epithelial cell) cells labeling MRP2 with purified ab172630 at 1/70 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
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All lanes : Anti-MRP2 antibody [EPR10998] (ab172630) at 1/1000 dilution
Lane 1 : Human fetal liver lysate
Lane 2 : HepG2 lysate
Lane 3 : A549 lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 174 kDa -
Intracellular flow cytometric analysis of permeabilized A549 cells usingab172630 at a 1/100 dilution (red) or a rabbit IgG (negative) (green).
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Immunocytochemistry/ Immunofluorescence analysis of A549 cells labeling MRP2 with ab172630 at a 1/250 dilution.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (13)
ab172630 被引用在 13 文献中.
- Hu C et al. circFARP1 enables cancer-associated fibroblasts to promote gemcitabine resistance in pancreatic cancer via the LIF/STAT3 axis. Mol Cancer 21:24 (2022). PubMed: 35045883
- Akiyama S et al. Drug metabolic activity is a critical cell-intrinsic determinant for selection of hepatocytes during long-term culture. Stem Cell Res Ther 13:104 (2022). PubMed: 35279203
- Zhu X et al. Ordered micropattern arrays fabricated by lung-derived dECM hydrogels for chemotherapeutic drug screening. Mater Today Bio 15:100274 (2022). PubMed: 35601895
- Wang W et al. Transcriptome and Gut Microbiota Profiling Analysis of ANIT-Induced Cholestasis and the Effects of Da-Huang-Xiao-Shi Decoction Intervention. Microbiol Spectr 10:e0324222 (2022). PubMed: 36409145
- Gao J et al. Curcumol increases the sensitivity of colon cancer to 5-FU by regulating Wnt/β-catenin signaling. Transl Cancer Res 10:2437-2450 (2021). PubMed: 35116559