Anti-MRG15抗体(ab183663)
Key features and details
- Rabbit polyclonal to MRG15
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-MRG15抗体
参阅全部 MRG15 一抗 -
描述
兔多克隆抗体to MRG15 -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rat, Rabbit, Rhesus monkey -
免疫原
Recombinant fragment within Human MRG15 aa 96-323. The exact sequence is proprietary.
Database link: Q9UBU8 -
阳性对照
- Huh7, Hep3B and mouse liver whole cell lysate/extract; HeLa cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab183663于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/500 - 1/3000. Predicted molecular weight: 41 kDa.
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ICC/IF |
1/100 - 1/1000.
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说明 |
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WB
1/500 - 1/3000. Predicted molecular weight: 41 kDa. |
ICC/IF
1/100 - 1/1000. |
靶标
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功能
Component of the NuA4 histone acetyltransferase (HAT) complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. The NuA4 complex ATPase and helicase activities seem to be, at least in part, contributed by the association of RUVBL1 and RUVBL2 with EP400. NuA4 may also play a direct role in DNA repair when directly recruited to sites of DNA damage. Also component of the mSin3A complex which acts to repress transcription by deacetylation of nucleosomal histones. Required for homologous recombination repair (HRR) and resistance to mitomycin C (MMC). Involved in the localization of PALB2, BRCA2 and RAD51, but not BRCA1, to DNA-damage foci. -
序列相似性
Belongs to the MRG family. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 10933 Human
- Entrez Gene: 21761 Mouse
- Entrez Gene: 627352 Mouse
- Entrez Gene: 100340614 Rabbit
- Entrez Gene: 300891 Rat
- Entrez Gene: 711357 Rhesus monkey
- Omim: 607303 Human
- SwissProt: Q9UBU8 Human
see all -
别名
- CG6363 antibody
- Eaf3 antibody
- Esa1p associated factor 3 homolog antibody
see all
图片
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All lanes : Anti-MRG15 antibody (ab183663) at 1/500 dilution
Lane 1 : Huh7 whole cell lysate/extract
Lane 2 : Hep3B whole cell lysate/extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 41 kDa10 % SDS-PAGE
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Anti-MRG15 antibody (ab183663) at 1/500 dilution + Mouse liver lysate/extract at 50 µg
Predicted band size: 41 kDa10 % SDS-PAGE
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Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells labeling MRG15 with ab183663 at 1/1000 dilution (green). The slide on the right is counterstained with Hoechst 33342 (blue).
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab183663 被引用在 1 文献中.
- Charoy C et al. Genetic specification of left-right asymmetry in the diaphragm muscles and their motor innervation. Elife 6:N/A (2017). WB ; Mouse . PubMed: 28639940