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Epigenetics and Nuclear Signaling Chromosome Structure Telomeres

Anti-Mre11抗体[12D7] (ab214)

  • Datasheet
  • SDS
Reviews (10) Submit a question References (71)

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Western blot - Anti-Mre11 antibody [12D7] (ab214)
  • Immunocytochemistry/ Immunofluorescence - Anti-Mre11 antibody [12D7] (ab214)
  • Flow Cytometry - Anti-Mre11 antibody [12D7] (ab214)
  • Western blot - Anti-Mre11 antibody [12D7] (ab214)
  • Western blot - Anti-Mre11 antibody [12D7] (ab214)
  • Immunocytochemistry/ Immunofluorescence - Anti-Mre11 antibody [12D7] (ab214)
  • Western blot - Anti-Mre11 antibody [12D7] (ab214)

Key features and details

  • Mouse monoclonal [12D7] to Mre11
  • Suitable for: Flow Cyt, ICC/IF, WB
  • Reacts with: Rat, Human
  • Isotype: IgG1

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概述

  • 产品名称

    Anti-Mre11抗体[12D7]
    参阅全部 Mre11 一抗
  • 描述

    小鼠单克隆抗体[12D7] to Mre11
  • 宿主

    Mouse
  • 经测试应用

    适用于: Flow Cyt, ICC/IF, WBmore details
  • 种属反应性

    与反应: Rat, Human
  • 免疫原

    Amino acids 182-582 of Mre11 expressed in E. coli.

  • 阳性对照

    • WB HEK-293T, A431, HeLa, HepG2, PC-12 whole cell lysate; ICC: HeLa cells.
  • 常规说明

    This product was changed from ascites to tissue culture supernatant on 10th April 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    pH: 7.40
    Constituent: 100% PBS
  • Concentration information loading...
  • 纯度

    Tissue culture supernatant
  • 克隆

    单克隆
  • 克隆编号

    12D7
  • 骨髓瘤

    NS1
  • 同种型

    IgG1
  • 轻链类型

    kappa
  • 研究领域

    • Epigenetics and Nuclear Signaling
    • Chromosome Structure
    • Telomeres
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Damage & Repair
    • Non Homol. End Joining
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Damage & Repair
    • Homologous Recomb.

相关产品

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
    • Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control (ab91353)
  • Recombinant Protein

    • Recombinant Human Mre11 protein (ab114277)

应用

Our Abpromise guarantee covers the use of ab214 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use 1-2µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ICC/IF 1/100 - 1/1000.
WB 1/500 - 1/3000. Detects a band of approximately 79 kDa (predicted molecular weight: 79 kDa).

(see Robinson et al).

靶标

  • 功能

    Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11A. RAD50 may be required to bind DNA ends and hold them in close proximity. This could facilitate searches for short or long regions of sequence homology in the recombining DNA templates, and may also stimulate the activity of DNA ligases and/or restrict the nuclease activity of MRE11A to prevent nucleolytic degradation past a given point. The complex may also be required for DNA damage signaling via activation of the ATM kinase. In telomeres the MRN complex may modulate t-loop formation.
  • 疾病相关

    Defects in MRE11A are a cause of ataxia telangiectasia-like disorder (ATLD) [MIM:604391]. ATLD is a disease with the same clinical feature than ataxia-telangiectasia but with a somewhat milder clinical course.
  • 序列相似性

    Belongs to the MRE11/RAD32 family.
  • 翻译后修饰

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位

    Nucleus. Localizes to discrete nuclear foci after treatment with genotoxic agents.
  • Target information above from: UniProt accession P49959 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 4361 Human
    • Entrez Gene: 64046 Rat
    • Omim: 600814 Human
    • SwissProt: P49959 Human
    • SwissProt: Q9JIM0 Rat
    • Unigene: 192649 Human
    • Unigene: 209040 Rat
    • 别名

      • AT like disease antibody
      • Ataxia telangiectasia disorder like antibody
      • ATLD antibody
      • DNA recombination and repair protein antibody
      • Double strand break repair protein MRE11A antibody
      • Double-strand break repair protein MRE11A antibody
      • endo/exonuclease Mre11 antibody
      • HNGS1 antibody
      • meiotic recombination (S. cerevisiae) 11 homolog A antibody
      • Meiotic recombination 11 homolog 1 antibody
      • meiotic recombination 11 homolog A (S. cerevisiae) antibody
      • Meiotic recombination 11 homolog A antibody
      • MmMRE11A antibody
      • Mre 11 antibody
      • MRE 11a antibody
      • MRE 11b antibody
      • MRE11 homolog 1 antibody
      • MRE11 homolog A antibody
      • MRE11 homolog double strand break repair nuclease antibody
      • MRE11 meiotic recombination 11 homolog A (S. cerevisiae) antibody
      • MRE11 meiotic recombination 11 homolog A antibody
      • MRE11_HUMAN antibody
      • MRE11A antibody
      • MRE11b antibody
      • OTTHUMP00000236830 antibody
      • OTTHUMP00000236831 antibody
      • OTTHUMP00000236832 antibody
      • OTTHUMP00000236833 antibody
      see all

    图片

    • Western blot - Anti-Mre11 antibody [12D7] (ab214)
      Western blot - Anti-Mre11 antibody [12D7] (ab214)
      All lanes : Anti-Mre11 antibody [12D7] (ab214) at 1/1000 dilution

      Lane 1 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
      Lane 2 : A431 (Human epidermoid carcinoma cell line) whole cell lysate
      Lane 3 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
      Lane 4 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate

      Lysates/proteins at 30 µg per lane.

      Secondary
      All lanes : anti-mouse IgG HRP-conjugated antibody

      Predicted band size: 79 kDa



      7.5% SDS-PAGE

    • Immunocytochemistry/ Immunofluorescence - Anti-Mre11 antibody [12D7] (ab214)
      Immunocytochemistry/ Immunofluorescence - Anti-Mre11 antibody [12D7] (ab214)

      Immunocytochemical analysis of, 4% paraformaldehyde-fixed at RT for 15 min, HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling Mre-11 (green) with ab214 at 1/200 dilution. Blue: Hoechst 33342 staining. Scale bar= 10 μm.

    • Flow Cytometry - Anti-Mre11 antibody [12D7] (ab214)
      Flow Cytometry - Anti-Mre11 antibody [12D7] (ab214)

      Overlay histogram showing HeLa cells stained with ab214 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab214, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was a goat anti-mouse DyLight® 488 (IgG, H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

      This image was generated using the ascites version of the product.

    • Western blot - Anti-Mre11 antibody [12D7] (ab214)
      Western blot - Anti-Mre11 antibody [12D7] (ab214)
      Anti-Mre11 antibody [12D7] (ab214) at 1/500 dilution + PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate at 30 µg

      Secondary
      Anti-mouse IgG HRP-conjugated antibody

      Developed using the ECL technique.

      Predicted band size: 79 kDa



      7.5% SDS-PAGE

      The signal was developed with Trident ECL plus-Enhanced.

      This image was generated using the ascites version of the product.

    • Western blot - Anti-Mre11 antibody [12D7] (ab214)
      Western blot - Anti-Mre11 antibody [12D7] (ab214)
      All lanes : Anti-Mre11 antibody [12D7] (ab214) at 1/1000 dilution

      Lane 1 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
      Lane 2 : Human Mre-11-transfected HEK-293T whole cell lysate

      Lysates/proteins at 30 µg per lane.

      Secondary
      All lanes : anti-mouse IgG HRP-conjugated antibody

      Predicted band size: 79 kDa



      This image was generated using the ascites version of the product.

      7.5% SDS-PAGE

    • Immunocytochemistry/ Immunofluorescence - Anti-Mre11 antibody [12D7] (ab214)
      Immunocytochemistry/ Immunofluorescence - Anti-Mre11 antibody [12D7] (ab214)Image supplied by Dr Domenico Delia, Istituto Nazionale Tumori, Italy.

      Indirect immunofluorescence to detect localisation of Mre11 in normal lymphoblastoid cells.
      (Panel D - negative control).

      This image was generated using the ascites version of the product.

    • Western blot - Anti-Mre11 antibody [12D7] (ab214)
      Western blot - Anti-Mre11 antibody [12D7] (ab214)This image is courtesy of an anonymous Abreview
      All lanes : Anti-Mre11 antibody [12D7] (ab214) at 1/500 dilution

      Lane 1 : Mouse breast cancer cell line - whole cell lysate. Transfected with vector control.
      Lane 2 : Mouse breast cancer cell line - whole cell lysate. Transfected with knockdown shRNA targeting Mre11 gene.

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : HRP-conjugated Goat anti-mouse IgG polyclonal at 1/1000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 79 kDa
      Observed band size: 75 kDa
      why is the actual band size different from the predicted?


      Exposure time: 1 minute


      This image was generated using the ascites version of the product.

      See Abreview

    实验方案

    • Flow cytometry protocols
    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    数据表及文件

    • Datasheet
    • SDS
  • 文献 (71)

    发表研究结果有使用 ab214?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab214 被引用在 71 文献中.

    • Mooser C  et al. Treacle controls the nucleolar response to rDNA breaks via TOPBP1 recruitment and ATR activation. Nat Commun 11:123 (2020). PubMed: 31913317
    • Onn L  et al. SIRT6 is a DNA double-strand break sensor. Elife 9:N/A (2020). PubMed: 31995034
    • Thuault S  et al. A proximity-labeling proteomic approach to investigate invadopodia molecular landscape in breast cancer cells. Sci Rep 10:6787 (2020). PubMed: 32321993
    • Wang J  et al. Elevated MRE11 expression associated with progression and poor outcome in prostate cancer. J Cancer 10:4333-4340 (2019). PubMed: 31413753
    • Nieminuszczy J  et al. EXD2 Protects Stressed Replication Forks and Is Required for Cell Viability in the Absence of BRCA1/2. Mol Cell 75:605-619.e6 (2019). PubMed: 31255466
    View all Publications for this product

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-10 of 10 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Mre11 antibody [12D7]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Pig Cell (LLC-PK1 cell line)
    Permeabilization
    Yes - 0.5% Triton-X 100
    Specification
    LLC-PK1 cell line
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.1% · Temperature: R.T°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    提交于 Feb 10 2016

    Immunohistochemistry (Frozen sections) abreview for Anti-Mre11 [12D7] antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Sample
    Human Tissue sections (Normal or gastric cancer tissues)
    Specification
    Normal or gastric cancer tissues
    Permeabilization
    Yes - 0.1% Triton X-100
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    提交于 Feb 20 2014

    Immunocytochemistry abreview for Anti-Mre11 [12D7] antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 23°C
    Sample
    Mouse Cultured Cells (Mouse embryonic fibroblasts)
    Specification
    Mouse embryonic fibroblasts
    Permeabilization
    Yes - 0.1% Triton X-100
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    提交于 Feb 19 2014

    Immunocytochemistry abreview for Anti-Mre11 [12D7] antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 23°C
    Sample
    Human Cultured Cells (Hela cell line)
    Specification
    Hela cell line
    Permeabilization
    Yes - 0.1% Triton X-100
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    提交于 Feb 18 2014

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Mre11 [12D7] antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 23°C
    Sample
    Human Cell (Human colon cancer cell lines)
    Specification
    Human colon cancer cell lines
    Permeabilization
    Yes - 0.3% Triton X-100
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    提交于 Oct 15 2013

    Western blot abreview for Anti-Mre11 antibody [12D7]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    20 µg
    Gel Running Conditions
    Reduced Denaturing (7.5)
    Sample
    Mouse Cell lysate - whole cell (Mouse breast cancer cell line)
    Specification
    Mouse breast cancer cell line
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Read More

    Abcam user community

    Verified customer

    提交于 Oct 15 2013

    Western blot abreview for Anti-Mre11 antibody [12D7]

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (Mouse embryonic fibroblasts)
    Loading amount
    15 µg
    Specification
    Mouse embryonic fibroblasts
    Gel Running Conditions
    Reduced Denaturing (10% Tris-Gly gel)
    Blocking step
    LI-COR® Odyssey® Blocking Buffer as blocking agent for 45 minute(s) · Concentration: 50% · Temperature: RT°C
    Read More

    Abcam user community

    Verified customer

    提交于 Nov 08 2011

    Western blot abreview for Anti-Mre11 antibody [12D7]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - nuclear (human cancer cells (colon, bladder))
    Loading amount
    20 µg
    Specification
    human cancer cells (colon, bladder)
    Gel Running Conditions
    Reduced Denaturing (4-12)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 19°C
    Read More

    Abcam user community

    Verified customer

    提交于 Apr 12 2011

    Western blot abreview for Anti-Mre11 antibody [12D7]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HeLa cells)
    Loading amount
    20 µg
    Specification
    HeLa cells
    Gel Running Conditions
    Reduced Denaturing (10% Tris-Gly gel)
    Blocking step
    LI-COR® Odyssey® Blocking Buffer as blocking agent for 45 minute(s) · Concentration: 50% · Temperature: RT°C
    Read More

    Abcam user community

    Verified customer

    提交于 Nov 17 2010

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Mre11 antibody [12D7]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Normal Bronchus)
    Specification
    Normal Bronchus
    Fixative
    Formaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: EDTA
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 20 minute(s) · Concentration: 5%
    Read More

    Abcam user community

    Verified customer

    提交于 Jun 15 2007

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