小鼠/大鼠FGF1 ELISA试剂盒(ab223587)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 2 pg/ml
- Range: 16 pg/ml - 1000 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Serum, Tissue Lysate, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse, Rat
概述
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产品名称
小鼠/大鼠FGF1 ELISA试剂盒
参阅全部 FGF1 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Brain tissue 5 2.7% 批次间 样品 n Mean SD CV% Brain tissue 3 3.7% -
样品类型
Cell culture supernatant, Urine, Serum, EDTA Plasma, Cit plasma, Tissue Lysate -
检测类型
Sandwich (quantitative) -
灵敏度
2 pg/ml -
范围
16 pg/ml - 1000 pg/ml -
回收率
特定样本回收率 样品类型 平均% 范围 Urine 111 95% - 135% Serum 116 111% - 119% Cell culture media 117 108% - 125% EDTA Plasma 105 102% - 109% Cit plasma 119 111% - 125% Tissue Lysate 118 105% - 126% -
检测时间
1h 30m -
实验步骤
One step assay -
种属反应性
与反应: Mouse, Rat -
产品概述
Mouse/Rat FGF1 ELISA Kit (ab223587) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of FGF1 protein in cit plasma, edta plasma, serum, tissue lysate, urine, and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse/Rat FGF1 with NULL NULL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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说明
FGF1, also known as Fibroblast Growth Factor 1 or FGF acidic, is an important activator for several signaling cascades with roles in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. It functions as potent mitogen in vitro. In the presence of heparin, FGF1 binds in a 1:2 stoichiometry with FGF1 receptor protein, causing the autophosphorylation and activation of the receptor. This complex then binds to integrin and recruits PTPN11 to begin FGF1 signaling. Because of its possible role in organ development and in tumor growth, FGF1 is an important subject of study for the treatment of diabetes and the diagnosis and treatment of cancer. Mouse and rat FGF1 are identical on an amino acid level.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
平台
Pre-coated microplate (12 x 8 well strips)
性能
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存放说明
Store at +4°C. Please refer to protocols. -
组件 1 x 96 tests 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent CPR 1 x 6ml 10X Mouse/Rat FGF1 Capture Antibody 1 x 600µl 10X Mouse/Rat FGF1 Detector Antibody 1 x 600µl Mouse/Rat FGF1 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent 25BS 1 x 20ml Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
研究领域
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功能
The heparin-binding fibroblast growth factors play important roles in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. They are potent mitogens in vitro. -
序列相似性
Belongs to the heparin-binding growth factors family. -
细胞定位
Secreted. Cytoplasm. Cytoplasm > cell cortex. Lacks a cleavable signal sequence. Within the cytoplasm, it is transported to the cell membrane and then secreted by a non-classical pathway that requires Cu(2+) ions and S100A13. Secreted in a complex with SYT1. - Information by UniProt
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别名
- Acidic fibroblast growth factor
- aFGF
- Beta endothelial cell growth factor
see all -
数据库链接
- Entrez Gene: 14164 Mouse
- Entrez Gene: 25317 Rat
- SwissProt: P61148 Mouse
- SwissProt: P61149 Rat
- Unigene: 241282 Mouse
- Unigene: 88013 Rat
相关产品
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Alternative Versions
图片
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SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Background-subtracted data values (mean +/- SD) are graphed.
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Background-subtracted data values (mean +/- SD) are graphed.
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Background-subtracted data values (mean +/- SD) are graphed.
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The concentrations of FGF1 were measured in duplicates, interpolated from the FGF1 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (citrate) 25% and plasma (EDTA) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
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The concentrations of FGF1 were measured in duplicates, interpolated from the FGF1 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 25%, plasma (citrate) 25%, plasma (EDTA) 50%, and RAW 264.7 supernatant 95%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
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Interpolated concentrations of native mouse/rat FGF1 in mouse and rat brain tissue extract based on a 31 or 63 μg/mL extract load, respectively. The concentrations of FGF1 were measured in duplicate and interpolated from the FGF standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean FGF1 concentration was determined to be 697 pg/mL in mouse brain tissue extract and 796 pg/mL in rat brain tissue extract.
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The concentrations of FGF1 were measured in duplicate and interpolated from the FGF1 standard curve and corrected for sample dilution. Undiluted samples are as follows: mouse urine 25%, rat urine 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
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Only 14% interference was observed with the highest dose of hFGF1.
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To learn more about the advantages of recombinant antibodies see here.
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab223587 被引用在 2 文献中.
- Ye K et al. In vitro study of decellularized rat tissues for nerve regeneration. Front Neurol 13:986377 (2022). PubMed: 36188412
- Fei J et al. Low concentration of rutin treatment might alleviate the cardiotoxicity effect of pirarubicin on cardiomyocytes via activation of PI3K/AKT/mTOR signaling pathway. Biosci Rep 39:N/A (2019). PubMed: 31138757