小鼠VEGF-B ELISA试剂盒(ab213897)
Key features and details
- Sensitivity: 2 pg/ml
- Range: 31.2 pg/ml - 2000 pg/ml
- Sample type: Cell culture supernatant, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
概述
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产品名称
小鼠VEGF-B ELISA试剂盒
参阅全部 VEGFB 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% 1 16 70pg/ml 4.13 = 5% 2 16 247pg/ml 20.17 = 7.5% 3 16 978pg/ml 48.9 = 5% 批次间 样品 n Mean SD CV% 1 24 69pg/ml 4.83 = 7% 2 24 246pg/ml 20.17 = 8.2% 3 24 1037pg/ml 59.1 = 5.7% -
样品类型
Cell culture supernatant, Serum, Hep Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
< 2 pg/ml -
范围
31.2 pg/ml - 2000 pg/ml -
检测时间
3h 30m -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Mouse -
产品概述
The Mouse VEGF-B Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213897) is designed for the quantitative detection of Mouse VEGF-B in cell culture supernatants, serum and plasma (heparin).
The ELISA kit is based on standard sandwich enzyme-linked immunosorbent assay technology. A monoclonal antibody from rat specific for VEGF-B has been precoated onto 96-well plates. Standards (Expression system for standard: sf21; Immunogen sequence: Q20-A207 and Q20-R148) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for VEGF-B is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse VEGF-B amount of sample captured in plate.
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说明
Vascular endothelial growth factor B also known as VEGF-B is a protein that, in humans, is encoded by the VEGF-B gene. VEGF-B is a growth factor that belongs to the vascular endothelial growth factor family, of which VEGF-A is the best-known member. In contrast to VEGF-A, VEGF-B plays a less pronounced role in the vascular system: Whereas VEGF-A is important for the formation of blood vessels, such as during development or in pathological conditions, VEGF-B seems to play a role only in the maintenance of newly formed blood vessels during pathological conditions. It also plays an important role on several types of neurons. It is important for the protection of neurons in the retina and the cerebral cortex during stroke and of motor neurons during motor neuron diseases such as amyotrophic lateral sclerosis.
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平台
Pre-coated microplate (12 x 8 well strips)
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 标识符 1 x 96 tests ABC Diluent Buffer Blue Cap 1 x 12ml Adhesive Plate Seal 4 units Antibody Diluent Buffer Green Cap 1 x 12ml Anti-Mouse VEGF-B coated Microplate (12 x 8 wells) 1 unit Avidin-Biotin-Peroxidase Complex (ABC) 1 x 100µl Biotinylated anti- Mouse VEGF-B antibody 1 x 100µl Lyophilized recombinant Mouse VEGF-B standard 2 vials Sample Diluent Buffer Green Cap 1 x 30ml TMB Color Developing Agent Black Cap 1 x 10ml TMB Stop Solution Yellow Cap 1 x 10ml Wash Buffer (25X) 1 x 20ml -
研究领域
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功能
Growth factor for endothelial cells. VEGF-B167 binds heparin and neuropilin-1 whereas the binding to neuropilin-1 of VEGF-B186 is regulated by proteolysis. -
组织特异性
Expressed in all tissues except liver. Highest levels found in heart, skeletal muscle and pancreas. -
序列相似性
Belongs to the PDGF/VEGF growth factor family. -
翻译后修饰
VEGF-B186 is O-glycosylated. -
细胞定位
Secreted. Secreted but remains associated to cells or to the extracellular matrix unless released by heparin. - Information by UniProt
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别名
- Vascular endothelial growth factor B
- Vascular endothelial growth factor-related factor
- VEGF B
see all -
数据库链接
- Entrez Gene: 22340 Mouse
- SwissProt: P49766 Mouse
- Unigene: 15607 Mouse
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab213897 被引用在 3 文献中.
- Elhady SS et al. Anticancer Effects of New Ceramides Isolated from the Red Sea Red Algae Hypnea musciformis in a Model of Ehrlich Ascites Carcinoma: LC-HRMS Analysis Profile and Molecular Modeling. Mar Drugs 20:N/A (2022). PubMed: 35049918
- Elhady SS et al. VEGFR-Mediated Cytotoxic Activity of Pulicaria undulata Isolated Metabolites: A Biological Evaluation and In Silico Study. Life (Basel) 11:N/A (2021). PubMed: 34440504
- Llorián-Salvador M et al. VEGF-B Is an Autocrine Gliotrophic Factor for Müller Cells under Pathologic Conditions. Invest Ophthalmol Vis Sci 61:35 (2020). PubMed: 32945843