Mouse brain tissue homogenates were made in T-PER protein extraction reagent. With a total protein concentration of 6000 ug/ml, TIMP2 could be detected in both 1:2 and 1:4 dilutions. The ELISA assay was performed according to the instructions. We used the overnight incubation approach.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Dec 22 2017