Key features and details
- Sensitivity: 80 pg/ml
- Range: 0.0823 ng/ml - 60 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
参阅全部 TIMP2 试剂盒
样品类型Cell culture supernatant, Serum, Plasma
灵敏度< 80 pg/ml
范围0.082 ng/ml - 60 ng/ml
特定样本回收率 样品类型 平均% 范围 Cell culture supernatant 95.36 82% - 105% Serum 92.65 80% - 112% Plasma 93.42 81% - 103%
实验步骤Multiple steps standard assay
Abcam's TIMP2 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse TIMP2 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Mouse TIMP2 coated on a 96-well plate. Standards and samples are pipetted into the wells and TIMP2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse TIMP2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TIMP2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
存放说明Store at -20°C. Please refer to protocols.
组件 1 x 96 tests 20X Wash Buffer 1 x 25ml 260X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Biotinylated anti-Mouse TIMP2 2 vials Recombinant Mouse TIMP2 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TIMP2 Microplate (12 x 8 wells) 1 unit TMB One-Step Substrate Reagent 1 x 12ml
相关性Matrix metalloproteinases (MMPs) are a family of enzymes that are responsible for the degradation of extracellular matrix components such as collagen, laminin and proteoglycans. These enzymes are involved in normal physiological processes such as embryogenesis and tissue remodeling and may play an important role in arthritis, periodontitis, and metastasis. The activation and activity of MMPs are regulated by a family of endogenous inhibitors, tissue inhibitors of metalloproteinase (TIMP). TIMP2 (also called CSC-21K) is a 24 kDa glycoprotein that is expressed by a variety of cell types. It forms a non covalent, stoichiometric complex with both latent and active MMPs. TIMP2 shows a preference for MMP2. TIMPs are capable of altering the metastatic potential of cancer cells and have been shown to inhibit invasion and metastasis in animal models. TIMP2 is constitutively produced in low (pg/ml) levels by most cell types in tissue culture. The low protein levels produced often require concentration of cell culture media to visualize the bands by Western Blotting.
- CSC 21K
ab100746 被引用在 2 文献中.
- Morad G et al. Cdc42-Dependent Transfer of mir301 from Breast Cancer-Derived Extracellular Vesicles Regulates the Matrix Modulating Ability of Astrocytes at the Blood-Brain Barrier. Int J Mol Sci 21:N/A (2020). PubMed: 32481745
- Tajerian M et al. The hippocampal extracellular matrix regulates pain and memory after injury. Mol Psychiatry 23:2302-2313 (2018). PubMed: 30254235