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Kits/ Lysates/ Other Lysates Tissue Lysates Mouse Other

小鼠spleen组织裂解物- total蛋白(ab7937)

  • Datasheet
  • SDS
Submit a review Q&A (1)References (2)

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概述

  • 产品名称

    小鼠spleen组织裂解物- total蛋白
    参阅全部 Spleen 细胞裂解液
  • 常规说明

    Source: White laboratory mice


    Mouse spleen tissue lysate was prepared by homogenization in modified RIPA buffer (150mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Tissue and cell debris were removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The tissue lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol.

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

  • 经测试应用

    适用于: WBmore details

性能

  • Mycoplasma free

    Yes
  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    pH: 7.2
    Constituents: 60.05% Water, 12.5% Glycerol (glycerin, glycerine), 9% Tris HCl, 7.7% DTT, 4.4% Sodium chloride, 1% Triton-X-100, 1% Sodium deoxycholate, 1.1% Sodium lauryl sulfate, 0.15% EDTA disodium salt, 0.5% Aprotinin, 0.5% Leupeptin hemisulfate, 0.09% PMSF, 0.01% Bromophenol blue
  • Concentration information loading...
  • 裂解物说明

    Mouse spleen tissue lysate was prepared by homogenization in modified RIPA buffer (150mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Tissue and cell debris were removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The tissue lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol.
  • 研究领域

    • Kits/ Lysates/ Other
    • Lysates
    • Tissue Lysates
    • Mouse
    • Other
    • Kits/ Lysates/ Other
    • Lysates
    • Whole Cell Lysates
    • Mouse
    • Other

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab7937于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
Use at an assay dependent concentration. Mouse spleen tissue lysate is ready to load on SDS-PAGE for Western blotting, 20 µg per lane is recommended for mini gel.
说明
WB
Use at an assay dependent concentration. Mouse spleen tissue lysate is ready to load on SDS-PAGE for Western blotting, 20 µg per lane is recommended for mini gel.

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

数据表及文件

  • SDS download

  • Datasheet download

    Download

文献 (2)

发表研究结果有使用 ab7937?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab7937 被引用在 2 文献中.

  • Wen X  et al. CircRNA-016901 silencing attenuates irradiation-induced injury in bone mesenchymal stem cells via regulating the miR-1249-5p/HIPK2 axis. Exp Ther Med 21:355 (2021). PubMed: 33732328
  • Yang Y  et al. Cryptotanshinone alleviates polycystic ovary syndrome in rats by regulating the HMGB1/TLR4/NF-?B signaling pathway. Mol Med Rep 22:3851-3861 (2020). PubMed: 32901834

客户评价及客户问答

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Question

Since we've got the antibody we were unable to have good results always having to many bands. We were trying to detect Ikaros in mouse fetal liver and added fetal liver from Ikaros knockout mice as a negatif control and we got the same bands as the wildtype.

We used frozen tissu (total protein extract), blocked the whole day in 5%milk and incubate ovenight with the Ikaros antibody ab26083 lot 808013 dilution 1:1000. the next day we washed 3 times with PBST and incubate 1 hour with the secondary antibody.

Do you think frozen protein extract are not good. does ikaros protein degrade fast?have you ever tested the antibody in mouse fetal liver? and even how did it work in the spleen

Thanks in advance for your help

Read More

Abcam community

Verified customer

Asked on Aug 02 2012

Answer

Thank you for your reply.

After checking the expression pattern of Ikaros, we would not expect this protein to be detectable in liver samples. As a positive control, I would recommend a spleen whole cell lysate prepared with RIPA buffer (such as ab7937), or a nuclear extract from spleen.

Regarding the multiple bands, there are six known isoforms of this protein ranging from 30-60 kDa. Additionally, there are a number of phosphorylation sites that could cause fluctuations in the observed band size.

In general, frozen samples should still be viable as long as they have not been stored for a very long time or exposed to multiple freeze-thaw cycles. We have not received any feedback indicating that this protein degrades readily, but I would still recommend preparing your lysates on ice in the presence of protease and phosphatase inhibitors.

I hope this helps, please let me know if you need any additional information or assistance.

Read More

Abcam Scientific Support

回复于 Aug 02 2012

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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