小鼠RAGE ELISA试剂盒(ab100738)
Key features and details
- Sensitivity: 15 pg/ml
- Range: 13.72 pg/ml - 10000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
概述
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产品名称
小鼠RAGE ELISA试剂盒
参阅全部 RAGE 试剂盒 -
检测方法
Colorimetric -
样品类型
Cell culture supernatant, Serum, Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
< 15 pg/ml -
范围
13.72 pg/ml - 10000 pg/ml -
回收率
87 %
特定样本回收率 样品类型 平均% 范围 Cell culture supernatant 75.89 67% - 82% Serum 102.9 91% - 110% Plasma 82.69 71% - 92% -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Mouse -
产品概述
Abcam’s RAGE Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse RAGE in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for mouse RAGE coated on a 96-well plate. Standards and samples are pipetted into the wells and RAGE present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse RAGE antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of RAGE bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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平台
Microplate
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 1 x 96 tests 160X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer 1 x 25ml 5X Assay Diluent 1 x 15ml Biotinylated anti-Mouse RAGE 2 vials RAGE Microplate (12 x 8 wells) 1 unit Recombinant Mouse RAGE Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
研究领域
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功能
Mediates interactions of advanced glycosylation end products (AGE). These are nonenzymatically glycosylated proteins which accumulate in vascular tissue in aging and at an accelerated rate in diabetes. Acts as a mediator of both acute and chronic vascular inflammation in conditions such as atherosclerosis and in particular as a complication of diabetes. AGE/RAGE signaling plays an important role in regulating the production/expression of TNF-alpha, oxidative stress, and endothelial dysfunction in type 2 diabetes. Interaction with S100A12 on endothelium, mononuclear phagocytes, and lymphocytes triggers cellular activation, with generation of key proinflammatory mediators. Interaction with S100B after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling (By similarity). Receptor for amyloid beta peptide. Contributes to the translocation of amyloid-beta peptide (ABPP) across the cell membrane from the extracellular to the intracellular space in cortical neurons. ABPP-initiated RAGE signaling, especially stimulation of p38 mitogen-activated protein kinase (MAPK), has the capacity to drive a transport system delivering ABPP as a complex with RAGE to the intraneuronal space. -
组织特异性
Endothelial cells. -
序列相似性
Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
细胞定位
Secreted and Cell membrane. - Information by UniProt
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别名
- Advanced glycosylation end product-specific receptor
- Ager
- DAMA 358M23.4
see all -
数据库链接
- Entrez Gene: 11596 Mouse
- SwissProt: Q62151 Mouse
- Unigene: 3383 Mouse
图片
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Standard curve of mouse RAGE with background signal subtracted (duplicates; +/- SD).
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Mouse RAGE measured in in mouse tissue lysates (in RIPA buffer tested in the range of 0.1ug to 0.1 mg protein, quantity of RAGE shown as per mg of extracted protein ), duplicates +/- SD.
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Mouse RAGE measured in biological fluids (dilution range neat - 1/10; duplicates +/- SD).
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Representative standard curve using ab100738
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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