小鼠proMMP9 ELISA试剂盒(ab100732)
Key features and details
- Sensitivity: 50 pg/ml
- Range: 74.07 pg/ml - 18000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
概述
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产品名称
小鼠proMMP9 ELISA试剂盒
参阅全部 MMP9 试剂盒 -
检测方法
Colorimetric -
样品类型
Cell culture supernatant, Serum, Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
< 50 pg/ml -
范围
74.07 pg/ml - 18000 pg/ml -
回收率
100 %
特定样本回收率 样品类型 平均% 范围 Cell culture supernatant 109.7 96% - 118% Serum 114.4 97% - 132% Plasma 112 101% - 121% -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Mouse -
产品概述
proMMP9 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit (ab100732) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse proMMP9 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for mouse proMMP9 coated on a 96-well plate. Standards and samples are pipetted into the wells and proMMP9 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse proMMP9 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of proMMP9 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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平台
Microplate
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 1 x 96 tests 20X Wash Buffer 1 x 25ml 400X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent 1 x 15ml Biotinylated anti-Mouse proMMP9 2 vials proMMP9 Microplate (12 x 8 wells) 1 unit Recombinant Mouse proMMP9 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
研究领域
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功能
May play an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly-
-Leu bond. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. Degrades fibronectin but not laminin or Pz-peptide. -
组织特异性
Produced by normal alveolar macrophages and granulocytes. -
疾病相关
Intervertebral disc disease
Metaphyseal anadysplasia 2 -
序列相似性
Belongs to the peptidase M10A family.
Contains 3 fibronectin type-II domains.
Contains 4 hemopexin repeats. -
结构域
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. -
翻译后修饰
Processing of the precursor yields different active forms of 64, 67 and 82 kDa. Sequentially processing by MMP3 yields the 82 kDa matrix metalloproteinase-9.
N- and O-glycosylated. -
细胞定位
Secreted, extracellular space, extracellular matrix. - Information by UniProt
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别名
- 82 kDa matrix metalloproteinase-9
- 92 kDa gelatinase
- 92 kDa type IV collagenase
see all -
数据库链接
- Entrez Gene: 17395 Mouse
- SwissProt: P41245 Mouse
数据表及文件
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SDS download
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Datasheet download
文献 (5)
ab100732 被引用在 5 文献中.
- Boodhoo K et al. Dysregulated healing responses in diabetic wounds occur in the early stages postinjury. J Mol Endocrinol 66:141-155 (2021). PubMed: 33350981
- Deng HJ et al. A20 Establishes Negative Feedback With TRAF6/NF-κB and Attenuates Early Brain Injury After Experimental Subarachnoid Hemorrhage. Front Immunol 12:623256 (2021). PubMed: 34381441
- Ma J et al. Rivaroxaban attenuates thrombosis by targeting the NF-?B signaling pathway in a rat model of deep venous thrombus. Int J Mol Med 40:1869-1880 (2017). PubMed: 29039441
- Yan B et al. HMGB1-TLR4-IL23-IL17A axis promotes paraquat-induced acute lung injury by mediating neutrophil infiltration in mice. Sci Rep 7:597 (2017). PubMed: 28377603
- Xie J et al. The effects of interleukin-1ß in modulating osteoclast-conditioned medium's influence on gelatinases in chondrocytes through mitogen-activated protein kinases. Int J Oral Sci 7:220-31 (2015). PubMed: 26674424