Key features and details
- Sensitivity: 14 pg/ml
- Range: 10.97 pg/ml - 8000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
参阅全部 CXCL7/PBP 试剂盒
样品类型Cell culture supernatant, Serum, Plasma
灵敏度< 14 pg/ml
范围10.97 pg/ml - 8000 pg/ml
特定样本回收率 样品类型 平均% 范围 Cell culture supernatant 123.5 108% - 133% Serum 118.3 100% - 135% Plasma 97.75 75% - 110%
实验步骤Multiple steps standard assay
Abcam’s PPBP Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse PPBP in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Mouse PPBP coated on a 96- well plate. Standards and samples are pipetted into the wells and PPBP present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse PPBP antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of PPBP bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
存放说明Store at -20°C. Please refer to protocols.
组件 1 x 96 tests 20X Wash Buffer 1 x 25ml 5X Assay Diluent B 1 x 15ml 600X HRP-Streptavidin Concentrate 1 x 200µl Assay Diluent C 1 x 30ml Biotinylated anti-Mouse PPBP 2 vials PPBP Microplate (12 x 8 wells) 1 unit Recombinant Mouse PPBP Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml
功能LA-PF4 stimulates DNA synthesis, mitosis, glycolysis, intracellular cAMP accumulation, prostaglandin E2 secretion, and synthesis of hyaluronic acid and sulfated glycosaminoglycan. It also stimulates the formation and secretion of plasminogen activator by human synovial cells. NAP-2 is a ligand for CXCR1 and CXCR2, and NAP-2, NAP-2(73), NAP-2(74), NAP-2(1-66), and most potent NAP-2(1-63) are chemoattractants and activators for neutrophils. TC-1 and TC-2 are antibacterial proteins, in vitro released from activated platelet alpha-granules. CTAP-III(1-81) is more potent than CTAP-III desensitize chemokine-induced neutrophil activation.
序列相似性Belongs to the intercrine alpha (chemokine CxC) family.
翻译后修饰Proteolytic removal of residues 1-9 produces the active peptide connective tissue-activating peptide III (CTAP-III) (low-affinity platelet factor IV (LA-PF4)).
Proteolytic removal of residues 1-13 produces the active peptide beta-thromboglobulin, which is released from platelets along with platelet factor 4 and platelet-derived growth factor.
NAP-2(1-66) is produced by proteolytical processing, probably after secretion by leukocytes other than neutrophils.
NAP-2(73) and NAP-2(74) seem not be produced by proteolytical processing of secreted precursors but are released in an active form from platelets.
- Information by UniProt
- B TG1
- Beta TG
- Beta thromboglobulin
- Entrez Gene: 57349 Mouse