小鼠PLGF-2 ELISA试剂盒(PGF) (ab155459)

概述

  • 产品名称
    小鼠PLGF-2 ELISA试剂盒(PGF)
  • 检测方法
    Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    overall < 10%
    批次间
    样品 n Mean SD CV%
    Overall < 12%
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型
    Sandwich (quantitative)
  • 灵敏度
    < 1 pg/ml
  • 范围
    1.64 pg/ml - 400 pg/ml
  • 回收率

    特定样本回收率
    样品类型 平均% 范围
    Cell culture supernatant 110.3 104% - 116%
    Serum 95.3 87% - 103%
    Plasma 91.02 74% - 107%

  • 实验步骤
    Multiple steps standard assay
  • 种属反应性
    与反应: Mouse
  • 产品概述

    Abcam’s PLGF-2 (PGF) Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay designed for the quantitative measurement of mouse PLGF-2 (PGF) in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for mouse PLGF-2 coated on a 96-well plate. Standards and samples are pipetted into the wells and PLGF-2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse PLGF-2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of PLGF-2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 经测试应用
    适用于: Sandwich ELISAmore details
  • 平台
    Microplate

性能

应用

Our Abpromise guarantee covers the use of ab155459 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

图片

  • Representative standard curve using ab155459 - Assay Diluent C.

  • Representative standard curve using ab155459 -  Assay Diluent B.

实验方案

文献

ab155459 has not yet been referenced specifically in any publications.

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