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小鼠单克隆抗体[hlgA1]抗人IgA H&L (ab433)

Submit a review Q&A (3)References (1)

Key features and details

  • Mouse monoclonal [hlgA1] Anti-Human IgA H&L
  • Host species: Mouse
  • Isotype: IgG1
  • Suitable for: ELISA, RIA

概述

  • 产品名称

    小鼠单克隆抗体[hlgA1]抗人IgA H&L
    参阅全部 IgA 二抗

  • 宿主

    Mouse

  • 靶标种属

    Human

  • 特异性

    hlgA1 specifically recognizes human IgA. hIgA - 100% hIgA (secretory) - 107% hIgA (myeloma) - 135% hIgA1 (myeloma) - 145% hIgA2 (myeloma) - 91% hIgA1 (myeloma) - 104% hIgA2 (myeloma) - 200% hIgA alpha-chain - 138% hIgG - <0.3% hIgG - <0.3% hIgM (plasma) - 2.6% hIgM (Waldenström's macroglobulinaemia) - <0.3% Affinity constant: 3x109 L/mol human plasma IgA.

  • 经测试应用

    适用于: ELISA, RIAmore details

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • 存储溶液

    pH: 6.00
    Preservative: 0.095% Sodium azide
    Constituents: 0.335% PBS, 1.0878% Sodium citrate, 0.9% Sodium chloride
  • Concentration information loading...

  • 纯度

    Protein A purified

  • 克隆

    单克隆

  • 克隆编号

    hlgA1

  • 同种型

    IgG1

  • 轻链类型

    unknown

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab433于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ELISA
Use at an assay dependent concentration.
RIA
Use at an assay dependent concentration.
说明
ELISA
Use at an assay dependent concentration.
RIA
Use at an assay dependent concentration.

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

文献 (1)

发表研究结果有使用 ab433？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab433 被引用在 1 文献中.

  • Juhela S  et al. T cell responses to enterovirus antigens and to beta-cell autoantigens in unaffected children positive for IDDM-associated autoantibodies. J Autoimmun 12:269-78 (1999). PubMed: 10330298

客户评价及客户问答

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1-3 of 3 Abreviews or Q&A

Question

Is there anti human IgA2 conjugate [ Monoclonal or Polyclonal]other than ICN ?

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Answer

Thank you for your enquiry. Ab 433 is the only IgA secondary antibody that we have. To see if another company may have what you're looking for, I suggest that you click on the link to "The World's Antibody Gateway". The World's Antibody Gateway is a free search engine service provided by Abcam to help you to quickly find the antibodies that you are looking for. It is a free-text search engine developed by the Abcam team so that it searches the catalogs of all online antibody companies (currently 249).

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Question

Will this reagent cross react with monkey IgA? I need a single reagent that will detect human and monkey IgA, but not other classes of Ig.

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Answer

We have not tested the cross reactivity of this antibody (Mouse monoclonal to human IgA) with monkey IgA or with other monkey Ig classes. Its cross reativity with human IgG is below 0.3% and with human IgM(plasma) 2.6%. Human IgM(Waldenström's macroglobulinemia) cross reacts less than 0.3%.

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Question

Will this antibody recognize secreted IgA in the saliva? Does it crossreact with rat sIgA?

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Answer

Anti-IgA (ab433) cross-reacted 107% with the secretory IgA preparation from DAKO. Cross reacivity with rat and saliva as a sample have not yet been tested.

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