小鼠单克隆抗体[2C11]抗人IgG1 Fc (ab1927)
概述
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产品名称
小鼠单克隆抗体[2C11]抗人IgG1 Fc
参阅全部 IgG1 二抗 -
宿主
Mouse -
靶标种属
Human -
经测试应用
适用于: IHC-Fr, ELISAmore details -
免疫原
IgG from human serum.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading... -
纯度
Protein A purified -
纯化说明
Chromatography on protein A Sepharose -
克隆
单克隆 -
克隆编号
2C11 -
骨髓瘤
Sp2/0 -
同种型
IgG1 -
常规说明
Concentration varies from lot to lot and can be provided on request. -
研究领域
应用
Our Abpromise guarantee covers the use of ab1927 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-Fr | Use at an assay dependent concentration. | |
| ELISA | Use at an assay dependent concentration. PubMed: 24373342 |
图片
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![ELISA - Mouse monoclonal [2C11] Anti-Human IgG1 Fc (ab1927)](https://www.abcam.cn/ps/products/1/ab1927/Images/ab1927-331323-mouse-monoclonal-2c11-anti-human-igg1-fc-elisa.jpg)
ELISA - Mouse monoclonal [2C11] Anti-Human IgG1 Fc (ab1927)Marino et al PLoS One. 2017 May 18;12(5):e0177810. doi: 10.1371/journal.pone.0177810. eCollection 2017. Fig 5. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/Characterisation of virus activity and functional expression of monoclonal antibodies.
(Panel B) IgG1 antibody expression detected in cellular supernatants of HT-29 cells infected with 10ppc NG-135 by anti-IgG1 ELISA.
Media was removed from infected or control cells, clarified by centrifugation and diluted 1 in 2 in 3% BSA/PBS for storage at -20°C. For quantification of human IgG1, 96 well plates (NUNC) were coated overnight, 4°C, with mouse monoclonal anti-human IgG1 Fc antibody (ab1927, Abcam) at a 1:1000 dilution in carbonate/bicarbonate buffer. Plates were blocked in 3% BSA/PBS, washed and samples and standards added to the plate for 1 hr and RT. Secondary detection was carried out using a HRP conjugated goat anti-human IgG Fab (ab87422, Abcam) incubated for 1 hr, RT.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
文献
ab1927 被引用在 8 文献中.
- Wang J et al. Krüppel like factor 6 splice variant 1 (KLF6-SV1) overexpression recruits macrophages to participate in lung cancer metastasis by up-regulating TWIST1. Cancer Biol Ther 20:680-691 (2019). PubMed: 30590988
- Zhao Z et al. Upregulation of Insulin-Like Growth Factor-1 Receptor (IGF-1R) Reverses the Inhibitory Effect of Let-7g-5p on Migration and Invasion of Nasopharyngeal Carcinoma. Med Sci Monit 25:5747-5756 (2019). PubMed: 31374070
- Wen Z et al. Inhibition of lung cancer cells and Ras/Raf/MEK/ERK signal transduction by ectonucleoside triphosphate phosphohydrolase-7 (ENTPD7). Respir Res 20:194 (2019). PubMed: 31443651
- Vanden Bempt M et al. Cooperative Enhancer Activation by TLX1 and STAT5 Drives Development of NUP214-ABL1/TLX1-Positive T Cell Acute Lymphoblastic Leukemia. Cancer Cell 34:271-285.e7 (2018). PubMed: 30107177
- Girardi T et al. The T-cell leukemia-associated ribosomal RPL10 R98S mutation enhances JAK-STAT signaling. Leukemia 32:809-819 (2018). Human . PubMed: 28744013
- Marino N et al. Development of a versatile oncolytic virus platform for local intra-tumoural expression of therapeutic transgenes. PLoS One 12:e0177810 (2017). PubMed: 28542292
- Medeiros MM et al. Natural antibody response to Plasmodium falciparum merozoite antigens MSP5, MSP9 and EBA175 is associated to clinical protection in the Brazilian Amazon. BMC Infect Dis 13:608 (2013). ELISA ; Human . PubMed: 24373342
- Hendaoui I et al. Inhibition of Wnt/ß-catenin signaling by a soluble collagen-derived frizzled domain interacting with Wnt3a and the receptors frizzled 1 and 8. PLoS One 7:e30601 (2012). PubMed: 22303445
