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Immunology Immunoglobulins Heavy Chain IgG

小鼠单克隆抗体[2C11]抗人IgG1 Fc (ab1927)

  • Datasheet
  • SDS
Reviews (1)Q&A (5)References (10)

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ELISA - Mouse monoclonal [2C11] Anti-Human IgG1 Fc (ab1927)

    Key features and details

    • Mouse monoclonal [2C11] Anti-Human IgG1 Fc
    • Host species: Mouse
    • Isotype: IgG1
    • Suitable for: IHC-Fr, ELISA

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    封闭
    Normal Mouse Serum (sterile) (ab7486)

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    概述

    • 产品名称

      小鼠单克隆抗体[2C11]抗人IgG1 Fc
      参阅全部 IgG1 二抗
    • 宿主

      Mouse
    • 靶标种属

      Human
    • 经测试应用

      适用于: IHC-Fr, ELISAmore details
    • 免疫原

      IgG from human serum.

    性能

    • 形式

      Liquid
    • 存放说明

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • 存储溶液

      pH: 7.40
      Preservative: 0.09% Sodium azide
      Constituent: PBS
    • Concentration information loading...
    • 纯度

      Protein A purified
    • 纯化说明

      Chromatography on protein A Sepharose
    • 克隆

      单克隆
    • 克隆编号

      2C11
    • 骨髓瘤

      Sp2/0
    • 同种型

      IgG1
    • 常规说明

      Concentration varies from lot to lot and can be provided on request.
    • 研究领域

      • Immunology
      • Immunoglobulins
      • Heavy Chain
      • IgG
      • Secondary antibodies
      • anti-Human
      • IgG
      • Unconjugated

    相关产品

      应用

      The Abpromise guarantee

      Our Abpromise guarantee covers the use of ab1927 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      应用 Ab评论 说明
      IHC-Fr
      Use at an assay dependent concentration.
      ELISA
      Use at an assay dependent concentration. PubMed: 24373342
      说明
      IHC-Fr
      Use at an assay dependent concentration.
      ELISA
      Use at an assay dependent concentration. PubMed: 24373342

      图片

      • ELISA - Mouse monoclonal [2C11] Anti-Human IgG1 Fc (ab1927)
        ELISA - Mouse monoclonal [2C11] Anti-Human IgG1 Fc (ab1927)Marino et al PLoS One. 2017 May 18;12(5):e0177810. doi: 10.1371/journal.pone.0177810. eCollection 2017. Fig 5. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

        Characterisation of virus activity and functional expression of monoclonal antibodies.

        (Panel B) IgG1 antibody expression detected in cellular supernatants of HT-29 cells infected with 10ppc NG-135 by anti-IgG1 ELISA.

        Media was removed from infected or control cells, clarified by centrifugation and diluted 1 in 2 in 3% BSA/PBS for storage at -20°C. For quantification of human IgG1, 96 well plates (NUNC) were coated overnight, 4°C, with mouse monoclonal anti-human IgG1 Fc antibody (ab1927, Abcam) at a 1:1000 dilution in carbonate/bicarbonate buffer. Plates were blocked in 3% BSA/PBS, washed and samples and standards added to the plate for 1 hr and RT. Secondary detection was carried out using a HRP conjugated goat anti-human IgG Fab (ab87422, Abcam) incubated for 1 hr, RT.

      实验方案

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      数据表及文件

      • SDS download

      • Datasheet download

        Download

      文献 (10)

      发表研究结果有使用 ab1927?请让我们知道,以便我们可以引用本数据表中的参考文章。

      ab1927 被引用在 10 文献中.

      • Wang J  et al. Krüppel like factor 6 splice variant 1 (KLF6-SV1) overexpression recruits macrophages to participate in lung cancer metastasis by up-regulating TWIST1. Cancer Biol Ther 20:680-691 (2019). PubMed: 30590988
      • Zhao Z  et al. Upregulation of Insulin-Like Growth Factor-1 Receptor (IGF-1R) Reverses the Inhibitory Effect of Let-7g-5p on Migration and Invasion of Nasopharyngeal Carcinoma. Med Sci Monit 25:5747-5756 (2019). PubMed: 31374070
      • Wen Z  et al. Inhibition of lung cancer cells and Ras/Raf/MEK/ERK signal transduction by ectonucleoside triphosphate phosphohydrolase-7 (ENTPD7). Respir Res 20:194 (2019). PubMed: 31443651
      • Zhang Y  et al. Sirtuin 6 overexpression relieves sepsis-induced acute kidney injury by promoting autophagy. Cell Cycle 18:425-436 (2019). PubMed: 30700227
      • Wu Z  et al. Hematopoietic-substrate-1 associated protein X-1 (HAX-1) regulates liver cancer cells growth, metastasis, and angiogenesis through Akt. Cancer Biol Ther 20:1223-1233 (2019). PubMed: 31132019
      • Girardi T  et al. The T-cell leukemia-associated ribosomal RPL10 R98S mutation enhances JAK-STAT signaling. Leukemia 32:809-819 (2018). Human . PubMed: 28744013
      • Vanden Bempt M  et al. Cooperative Enhancer Activation by TLX1 and STAT5 Drives Development of NUP214-ABL1/TLX1-Positive T Cell Acute Lymphoblastic Leukemia. Cancer Cell 34:271-285.e7 (2018). PubMed: 30107177
      • Marino N  et al. Development of a versatile oncolytic virus platform for local intra-tumoural expression of therapeutic transgenes. PLoS One 12:e0177810 (2017). PubMed: 28542292
      • Medeiros MM  et al. Natural antibody response to Plasmodium falciparum merozoite antigens MSP5, MSP9 and EBA175 is associated to clinical protection in the Brazilian Amazon. BMC Infect Dis 13:608 (2013). ELISA ; Human . PubMed: 24373342
      • Hendaoui I  et al. Inhibition of Wnt/ß-catenin signaling by a soluble collagen-derived frizzled domain interacting with Wnt3a and the receptors frizzled 1 and 8. PLoS One 7:e30601 (2012). PubMed: 22303445

      客户评价及客户问答

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      1-6 of 6 Abreviews or Q&A

      Mouse monoclonal 2C11 Anti-Human IgG1 Fc

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Flow Cytometry
      Growing cells on plates coated with Fc-DII4, differentiating to proT cells through staining with CD44 and CD25.
      Analyzed with FloCyt
      We have been using this antibody for years and have used 5 different batches.
      They have always done as promised in the datasheet.

      Abcam user community

      Verified customer

      提交于 Apr 17 2017

      Question

      I am looking for a secondary antibody, which detects human IgG1 specifically without cross reactivity towards murine Fc. I would like to use such antibody in a FACS staining and the labeling of the antibody does not matter.
      Does your company provide such antibody. From the data sheet it is hard to judge because it always says that the antibody might cross react with other IgGs.
      I would be very happy if you could get back to me.

      Kind regards,

      Read More

      Abcam community

      Verified customer

      Asked on Jun 11 2012

      Answer

      Thank you for contacting us.

      ab1927 does not cross react with mouse IgGs so it could also be a option to try.

      I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

      Use our products? Submit an Abreview. Earn rewards!
      https://www.abcam.com/abreviews

      Read More

      Abcam Scientific Support

      回复于 Jun 11 2012

      Question

      I am looking for a secondary antibody, which detects human IgG1 specifically without cross reactivity towards murine Fc. I would like to use such antibody in a FACS staining and the labeling of the antibody does not matter.
      Does your company provide such antibody. From the data sheet it is hard to judge because it always says that the antibody might cross react with other IgGs.
      I would be very happy if you could get back to me.

      Kind regards,

      Read More

      Abcam community

      Verified customer

      Asked on Jun 08 2012

      Answer

      Thank you for contacting us.

      Antibody ab50473 does not react with mouse IgGs. This might be suitable as per your requirements.

      I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

      Use our products? Submit an Abreview. Earn rewards!
      https://www.abcam.com/abreviews

      Read More

      Abcam Scientific Support

      回复于 Jun 08 2012

      Question

      I am looking for a secondary antibody, which detects human IgG1 specifically without cross reactivity towards murine Fc. I would like to use such antibody in a FACS staining and the labeling of the antibody does not matter.
      Does your company provide such antibody. From the data sheet it is hard to judge because it always says that the antibody might cross react with other IgGs.
      I would be very happy if you could get back to me.

      Kind regards,

      Read More

      Abcam community

      Verified customer

      Asked on Jun 08 2012

      Answer

      Thank you for contacting us.

      It seems product ab1927 and ab50473, could be suitable however in order to confirm it I am now waiting, species cross reactivity data from lab. I will get back to you with more updates soon.

      I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

      Use our products? Submit an Abreview. Earn rewards!
      https://www.abcam.com/abreviews

      Read More

      Abcam Scientific Support

      回复于 Jun 08 2012

      Question

      Hello,

      I am curious if any of the mouse anti-human IgG Mabs listed below cross react with monkey IgG?

      Mouse anti-Human IgG Fc secondary antibody (ab99757)

      Mouse monoclonal [H2] SecondaryAntibody to Human IgG - Fc (ab99770)

      Mouse anti-Human IgG1 Fc secondary antibody (ab1927)

      Mouse anti-Human IgG heavy chain secondary antibody (ab9243)

      Mouse monoclonal [hIgG1] SecondaryAntibody to Human IgG - H&L, pre-adsorbed (ab436)

      Read More

      Abcam community

      Verified customer

      Asked on Mar 19 2012

      Answer

      Thank you for contacting Abcam.

      We have not tested any of those antibodies against monkey IgG, therefore I cannot say whether they will cross react or not. Due to this, you can take advantage of our testing discount program if you want, please see the link below for more details:

      https://www.abcam.com/index.html?pageconfig=resource&rid=11998&viapagetrap=collaborationdiscount



      If there is anything else I can help you with, please let me know.

      Read More

      Abcam Scientific Support

      回复于 Mar 19 2012

      Question

      Is it IgG1, kappa or IgG1, lambda?

      Read More

      Abcam community

      Verified customer

      Asked on Apr 21 2005

      Answer

      Ab1927 - (Monoclonal mouse anti-human IgG1) - recognizes both kappa and lambda allotypes of human IgG1. Itself is isotype IgG1, and we have not determined if it is kappa or lambda allotype.

      Read More

      Abcam Scientific Support

      回复于 Apr 25 2005

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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