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小鼠MMP3 ELISA试剂盒(ab100731)

Submit a review Q&A (1)References (1)
Typical standard curve

    Key features and details

    • Sensitivity: 10 pg/ml
    • Range: 13.72 pg/ml - 10000 pg/ml
    • Sample type: Cell culture supernatant, Plasma, Serum
    • Detection method: Colorimetric
    • Assay type: Sandwich (quantitative)
    • Reacts with: Mouse

    概述

    • 产品名称

      小鼠MMP3 ELISA试剂盒
      参阅全部 MMP3 试剂盒

    • 检测方法

      Colorimetric

    • 样品类型

      Cell culture supernatant, Serum, Plasma

    • 检测类型

      Sandwich (quantitative)

    • 灵敏度

      < 10 pg/ml

    • 范围

      13.72 pg/ml - 10000 pg/ml

    • 回收率

      100 %

      特定样本回收率
      样品类型 平均% 范围
      Cell culture supernatant 117.4 106% - 130%
      Serum 120.6 101% - 140%
      Plasma 111.6 101% - 137%

    • 实验步骤

      Multiple steps standard assay

    • 种属反应性

      与反应: Mouse

    • 产品概述

      Abcam’s MMP3 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse MMP3 in serum, plasma and cell culture supernatants.

      This assay employs an antibody specific for mouse MMP3 coated on a 96-well plate. Standards and samples are pipetted into the wells and MMP3 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse MMP3 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MMP3 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

    • 平台

      Microplate

    性能

    图片

    • Typical standard curve
      Typical standard curve

      Representative standard curve using ab100731

    文献 (1)

    发表研究结果有使用 ab100731？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab100731 被引用在 1 文献中.

    • Yamakawa M  et al. Porphyromonas gingivalis infection exacerbates the onset of rheumatoid arthritis in SKG mice. Clin Exp Immunol 186:177-189 (2016). PubMed: 27465496

    客户评价及客户问答

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    Question

    To whom it may concern,   I have a question regarding your Mouse MMP3 ELISA kit (ab100731).  On the datasheet regarding the kit, it states: “Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases.”   I was wondering, does this ELISA recognize the general MMP3 protein, both inactive and active, or does it just recognize the activated, cleaved protein?   Appreciate your help!

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    Answer

    ab100731 detects both the pro and mature form.   I hope that helps! Please let me know if you have any more questions.

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