This is a relatively straightforward ELISA kit that can be done in 1 longish day or over 2 days. It is quite simple, except for the following elements that were a bit inconvenient:
1. Storage: -20degree storage, with many of the sample frozen and re-thawed in case you want to use the kit over 2 experiments (separate tubes would have fixed this).
2. Awkward concentrations: I was surprised to see that I had to diluted some things 400X. It's a bit strange and yields larger volumes that will be ultimately wasted...
3. The wells are removable, which is great if you want to run more than one experiment. However, they don't fit properly on the frame and easily come off when blotting it after washes. Very frustrating and not something I've seen with the other abcam ELISA kits that I've tried...
1. Storage: -20degree storage, with many of the sample frozen and re-thawed in case you want to use the kit over 2 experiments (separate tubes would have fixed this).
2. Awkward concentrations: I was surprised to see that I had to diluted some things 400X. It's a bit strange and yields larger volumes that will be ultimately wasted...
3. The wells are removable, which is great if you want to run more than one experiment. However, they don't fit properly on the frame and easily come off when blotting it after washes. Very frustrating and not something I've seen with the other abcam ELISA kits that I've tried...
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Aug 01 2018