小鼠MIP3 alpha ELISA试剂盒(CCL20) (ab100728)
Key features and details
- Sensitivity: 3 pg/ml
- Range: 2.06 pg/ml - 1500 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
概述
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产品名称
小鼠MIP3 alpha ELISA试剂盒(CCL20)
参阅全部 Macrophage Inflammatory Protein 3 alpha 试剂盒 -
检测方法
Colorimetric -
样品类型
Cell culture supernatant, Serum, Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
< 3 pg/ml -
范围
2.06 pg/ml - 1500 pg/ml -
回收率
90 %
特定样本回收率 样品类型 平均% 范围 Cell culture supernatant 80.52 72% - 91% Serum 83.74 74% - 93% Plasma 108.2 84% - 143% -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Mouse -
产品概述
Abcam’s MIP3 alpha Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse MIP3 alpha in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for mouse MIP3 alpha coated on a 96-well plate. Standards and samples are pipetted into the wells and MIP3 alpha present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse MIP3 alpha antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MIP3 alpha bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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平台
Microplate
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 1 x 96 tests 20X Wash Buffer 1 x 25ml 400X HRP-Streptavidin Concentrate 1 unit 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Biotinylated anti-mouse MIP3 alpha 2 vials MIP3 alpha Microplate (12 x 8 wells) 1 unit Recombinant Mouse MIP3 alpha Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
研究领域
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功能
Chemotactic factor that attracts lymphocytes and, slightly, neutrophils, but not monocytes. Inhibits proliferation of myeloid progenitors in colony formation assays. May be involved in formation and function of the mucosal lymphoid tissues by attracting lymphocytes and dendritic cells towards epithelial cells. C-terminal processed forms have been shown to be equally chemotactically active for leukocytes. Possesses antibacterial activity E.coli ATCC 25922 and S.aureus ATCC 29213. -
组织特异性
Expressed predominantly in the liver, lymph nodes, appendix, peripheral blood lymphocytes, and fetal lung. Low levels seen in thymus, prostate, testis, small intestine and colon. -
序列相似性
Belongs to the intercrine beta (chemokine CC) family. -
翻译后修饰
C-terminal processed forms which lack 1, 3 or 6 amino acids are produced by proteolytic cleavage after secretion from peripheral blood monocytes. -
细胞定位
Secreted. - Information by UniProt
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别名
- Beta chemokine exodus 1
- Beta-chemokine exodus-1
- C C motif chemokine ligand 20
see all -
数据库链接
- Entrez Gene: 20297 Mouse
- SwissProt: O89093 Mouse
图片
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Typical standard curve
Representative standard curve using ab100728
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Typical standard curveRepresentative standard curve using ab100728
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab100728 尚未被引用在任何文献中。