Key features and details
- Sensitivity: 2 pg/ml
- Range: 2.46 pg/ml - 600 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
样品类型Cell culture supernatant, Serum, Plasma
灵敏度< 2 pg/ml
范围2.46 pg/ml - 600 pg/ml
特定样本回收率 样品类型 平均% 范围 Cell culture supernatant 74.3 67% - 87% Serum 109.8 98% - 118% Plasma 76.8 67% - 93%
实验步骤Multiple steps standard assay
Abcam’s IL-28 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse IL-28 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for IL-28 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-28 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse IL-28 antibody is added. After washing away unbound biotinylated antibody, HRP conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-28 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
存放说明Store at -20°C. Please refer to protocols.
组件 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer 1 x 25ml 5X Assay Diluent B 1 x 15ml Assay Diluent C 1 x 30ml Biotinylated anti-Mouse IL-28 2 vials IL-28 Microplate (12 x 8 wells) 1 unit Recombinant Mouse IL-28 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml
- Cytokine Zcyto20
- IFN lambda 2
ab100708 被引用在 1 文献中.
- Okamoto M et al. IPS-1 is essential for type III IFN production by hepatocytes and dendritic cells in response to hepatitis C virus infection. J Immunol 192:2770-7 (2014). PubMed: 24532585