Key features and details
- Sensitivity: 45 pg/ml
- Range: 78.13 pg/ml - 5000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
参阅全部 IL-10 试剂盒
样品类型Cell culture supernatant, Serum, Plasma
灵敏度< 45 pg/ml
范围78.13 pg/ml - 5000 pg/ml
特定样本回收率 样品类型 平均% 范围 Cell culture supernatant 103.7 79% - 120% Serum 85.97 77% - 93% Plasma 92.2 71% - 134%
实验步骤Multiple steps standard assay
Abcam’s Mouse IL-10 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of IL-10 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for mouse IL-10 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-10 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse IL-10 antibody is added. After washing away unbound biotinylated antibody, HRP conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-10 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
存放说明Store at -20°C. Please refer to protocols.
组件 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer 1 x 25ml 5X Assay Diluent B 1 x 15ml 5X Assay Diluent D 1 x 15ml Biotinylated anti-Mouse IL-10 2 vials IL-10 Microplate (12 x 8 wells) 1 unit Recombinant Mouse IL-10 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml
功能Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells.
组织特异性Produced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types.
序列相似性Belongs to the IL-10 family.
- Information by UniProt
- Cytokine synthesis inhibitory factor
ab100697 被引用在 12 文献中.
- Mohamed ME et al. Yarrow oil ameliorates ulcerative colitis in mice model via regulating the NF-?B and PPAR-? pathways. Intest Res 19:194-205 (2021). PubMed: 32819032
- Wu CF et al. The Anti-Cancer Effects of a Zotarolimus and 5-Fluorouracil Combination Treatment on A549 Cell-Derived Tumors in BALB/c Nude Mice. Int J Mol Sci 22:N/A (2021). PubMed: 33925400
- Wu J et al. Nanostructured Zirconia Surfaces Regulate Human Gingival Fibroblasts Behavior Through Differential Modulation of Macrophage Polarization. Front Bioeng Biotechnol 8:611684 (2020). PubMed: 33553117
- Piao X et al. Picroside II Improves Severe Acute Pancreatitis-Induced Intestinal Barrier Injury by Inactivating Oxidative and Inflammatory TLR4-Dependent PI3K/AKT/NF-?B Signaling and Improving Gut Microbiota. Oxid Med Cell Longev 2020:3589497 (2020). PubMed: 32351672
- Gaitantzi H et al. BMP-9 Modulates the Hepatic Responses to LPS. Cells 9:N/A (2020). PubMed: 32143367