小鼠IgM [B11/7] -同型对照(ab91545)
概述
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产品名称
小鼠IgM [B11/7] -同型对照
参阅全部 Mouse 亚类对照 -
特异性
ab91545 was produced against a synthetic hapten, which is normally not present in Humans or animals. Therefore, ab91545 does not react with any antigen of Human or animal cells. -
经测试应用
适用于: Flow Cytmore details -
免疫原
Vegetal protein
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. Store In the Dark. -
存储溶液
pH: 7.20
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading...
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纯度
Protein G purified -
克隆
单克隆 -
克隆编号
B11/7 -
同种型
IgM -
研究领域
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别名
- Mouse Isotype Control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab91545于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Flow Cyt |
Use 2µg for 106 cells. ab91545 is a negative isotype control
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说明 |
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Flow Cyt
Use 2µg for 106 cells. ab91545 is a negative isotype control |
图片
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Overlay histogram showing A549 cells stained with ab2784 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2784, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgM [ICIGM] (ab91545, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in A549 cells fixed with 80% methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab91545 被引用在 2 文献中.
- Fáberová V et al. Super-Resolution Localisation of Nuclear PI(4)P and Identification of Its Interacting Proteome. Cells 9:N/A (2020). PubMed: 32403279
- Zhang CW et al. Long non-coding RNA PVT1 knockdown suppresses fibroblast-like synoviocyte inflammation and induces apoptosis in rheumatoid arthritis through demethylation of sirt6. J Biol Eng 13:60 (2019). PubMed: 31303891