小鼠IgM [B11/7] -同型对照(ab91545)

Q&A (3)References (1)

概述

  • 产品名称

    小鼠IgM [B11/7] -同型对照
    参阅全部 Mouse 亚类对照

  • 特异性

    ab91545 was produced against a synthetic hapten, which is normally not present in Humans or animals. Therefore, ab91545 does not react with any antigen of Human or animal cells.

  • 经测试应用

    适用于: Flow Cytmore details

  • 免疫原

    Vegetal protein

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C. Store In the Dark.

  • 存储溶液

    Preservative: 0.1% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.2
  • Concentration information loading...

  • 纯度

    Protein G purified

  • 克隆

    单克隆

  • 克隆编号

    B11/7

  • 同种型

    IgM

  • 研究领域

  • 别名

    • Mouse Isotype Control

应用

Our Abpromise guarantee covers the use of ab91545 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use 2µg for 106 cells. ab91545 is a negative isotype control

图片

  • Flow Cytometry - Mouse IgM [B11/7] - Isotype control (ab91545)
    Flow Cytometry - Mouse IgM [B11/7] - Isotype control (ab91545)

    Overlay histogram showing A549 cells stained with ab2784 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2784, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgM [ICIGM] (ab91545, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in A549 cells fixed with 80% methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

文献

This product has been referenced in:

  • Zhang CW  et al. Long non-coding RNA PVT1 knockdown suppresses fibroblast-like synoviocyte inflammation and induces apoptosis in rheumatoid arthritis through demethylation of sirt6. J Biol Eng 13:60 (2019). Read more (PubMed: 31303891) »
See 1 Publication for this product

发表研究结果有使用 ab91545？请让我们知道，以便我们可以引用本数据表中的参考文章。

客户评价及客户问答

Q&A
提交问题

1-3 of 3 Q&A

Question

The CD3 antibody that I have here is not from ABCAM.

I am looking for a negative control IgM, to be used in immunhistochemistry slides.

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Answer

Thank you for contacting us.

We carry several isotype controls that are mouse IgM:

ab91545: https://www.abcam.com/Mouse-IgM-ICIGM-Isotype-control-ab91545.html

ab18400: https://www.abcam.com/Mouse-IgM-kappa-MM-30-low-endotoxin-isotype-control-ab18400.html

ab18401: https://www.abcam.com/Mouse-IgM-kappa-monoclonal-MM-30-isotype-control-ab18401.html

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Question

What are the differences between then? I saw that same are Kappa ans one is ICIGM?





































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Answer

Thank you for your reply.

All 3 isotype controls are mouse monoclonal antibodies.

Two of them have a kappa light chain (ab18400 and ab18401). For ab91545, we do not have information about the type of light chain (either kappa or lambda).

ICIGM is the clone number. All clone numbers are given in brackets [...] in our catalog.

The antibodiesdiffer in the tested applications, concentration and buffer composition and amount you would receive (100 ug or 500 ug).

You might want to choose the one of these 3 controls that comes closest to your primary antibody in terms of concentration, but in general any of these isotype controls would be suitable for your IHC experiment.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Question

One of our customers wishes to test ab91545 as an isotype control for antibody ab49945 in IHC-P application. Would you like the customer to order and test the antibody as part of the abreview promotion?

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Answer

The testing discount promotion only applies to primary antibodies, defined as antibodies that are expected to produce a signal of some sort. For isotype controls such as ab91545, we will offer our guarantee for IHC, even though it is not listed on the datasheet, and so we do not offer the testing discount. If you or your customer have any questions, please contact us.

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