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小鼠IgM [B11/7] -同型对照(ab91545)

Q&A (3)References (2)
Flow Cytometry - Mouse IgM [B11/7] - Isotype control (ab91545)

    概述

    • 产品名称

      小鼠IgM [B11/7] -同型对照
      参阅全部 Mouse 亚类对照

    • 特异性

      ab91545 was produced against a synthetic hapten, which is normally not present in Humans or animals. Therefore, ab91545 does not react with any antigen of Human or animal cells.

    • 经测试应用

      适用于: Flow Cytmore details

    • 免疫原

      Vegetal protein

    • 常规说明

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    • 形式

      Liquid

    • 存放说明

      Shipped at 4°C. Store at +4°C. Store In the Dark.

    • 存储溶液

      pH: 7.20
      Preservative: 0.1% Sodium azide
      Constituents: PBS, 1% BSA
    • Concentration information loading...

    • 纯度

      Protein G purified

    • 克隆

      单克隆

    • 克隆编号

      B11/7

    • 同种型

      IgM

    • 研究领域

    • 别名

      • Mouse Isotype Control

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab91545于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    Flow Cyt
    Use 2µg for 106 cells. ab91545 is a negative isotype control
    说明
    Flow Cyt
    Use 2µg for 106 cells. ab91545 is a negative isotype control

    图片

    • Flow Cytometry - Mouse IgM [B11/7] - Isotype control (ab91545)
      Flow Cytometry - Mouse IgM [B11/7] - Isotype control (ab91545)

      Overlay histogram showing A549 cells stained with ab2784 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2784, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgM [ICIGM] (ab91545, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in A549 cells fixed with 80% methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

    文献 (2)

    发表研究结果有使用 ab91545？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab91545 被引用在 2 文献中.

    • Fáberová V  et al. Super-Resolution Localisation of Nuclear PI(4)P and Identification of Its Interacting Proteome. Cells 9:N/A (2020). PubMed: 32403279
    • Zhang CW  et al. Long non-coding RNA PVT1 knockdown suppresses fibroblast-like synoviocyte inflammation and induces apoptosis in rheumatoid arthritis through demethylation of sirt6. J Biol Eng 13:60 (2019). PubMed: 31303891

    客户评价及客户问答

    Q&A
    提交问题

    1-3 of 3 Q&A

    Question

    The CD3 antibody that I have here is not from ABCAM.

    I am looking for a negative control IgM, to be used in immunhistochemistry slides.

    Read More
    Answer

    Thank you for contacting us.

    We carry several isotype controls that are mouse IgM:

    ab91545: https://www.abcam.com/Mouse-IgM-ICIGM-Isotype-control-ab91545.html

    ab18400: https://www.abcam.com/Mouse-IgM-kappa-MM-30-low-endotoxin-isotype-control-ab18400.html

    ab18401: https://www.abcam.com/Mouse-IgM-kappa-monoclonal-MM-30-isotype-control-ab18401.html

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Question

    What are the differences between then? I saw that same are Kappa ans one is ICIGM?





































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    Read More
    Answer

    Thank you for your reply.

    All 3 isotype controls are mouse monoclonal antibodies.

    Two of them have a kappa light chain (ab18400 and ab18401). For ab91545, we do not have information about the type of light chain (either kappa or lambda).

    ICIGM is the clone number. All clone numbers are given in brackets [...] in our catalog.

    The antibodiesdiffer in the tested applications, concentration and buffer composition and amount you would receive (100 ug or 500 ug).

    You might want to choose the one of these 3 controls that comes closest to your primary antibody in terms of concentration, but in general any of these isotype controls would be suitable for your IHC experiment.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Question

    One of our customers wishes to test ab91545 as an isotype control for antibody ab49945 in IHC-P application. Would you like the customer to order and test the antibody as part of the abreview promotion?

    Read More
    Answer

    The testing discount promotion only applies to primary antibodies, defined as antibodies that are expected to produce a signal of some sort. For isotype controls such as ab91545, we will offer our guarantee for IHC, even though it is not listed on the datasheet, and so we do not offer the testing discount. If you or your customer have any questions, please contact us.

    Read More

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