重组小鼠IgG2c单克隆抗体[R313-MouseIgG2c]-Isotype control (ab280756)
Related conjugates and formulations
概述
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产品名称
小鼠IgG2c单克隆抗体[R313-MouseIgG2c]-Isotype control -
经测试应用
适用于: Flow Cyt (Intra), ICC/IFmore details
不适用于: IHC-P -
免疫原
The details of the immunogen for this antibody are not available.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Ion Exchange Chromatography -
克隆
单克隆 -
克隆编号
R313-MouseIgG2c -
同种型
IgG2c
相关产品
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Alternative Versions
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Compatible Secondaries
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Dyes/Markers
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Isotype control
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab280756于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Flow Cyt (Intra) |
1/1000.
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ICC/IF |
1/80.
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说明 |
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Flow Cyt (Intra)
1/1000. |
ICC/IF
1/80. |
图片
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Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized MOLT-4 cells labelling Mouse IgG2c with ab280756 at 1/80 dilution, followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594)at 1/500 dilution (Red). The nuclear counterstain was DAPI (Blue).
Confocal image showing no staining in MOLT-4 cells.
Negative control 1: ab280756 at a 1/80 dilution followed by ab150080 at a 1/200 dilution.
Negative control 2: ab179513 at a 1/200 dilution followed by ab150113 at a 1/1000 dilution.
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Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized MOLT-4 (Human lymphoblastic leukemia T lymphoblast) cells labelling Mouse IgG2c with ab280756 at 1/1000 dilution (0.1µg) (Red) compared with a Mouse IgG2c, kappa monoclonal [18C8BC7AD10] - Isotype Control (ab170191, Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
A Goat anti mouse IgG (Alexa Fluor® 488, ab150113) at 1/2000 dilution was used as the secondary antibody.
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Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized NIH/3T3 cells labelling Mouse IgG2c with ab280756 at 1/80 dilution, followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594)at 1/500 dilution (Red). The nuclear counterstain was DAPI (Blue).
Confocal image showing no staining in NIH/3T3 cells.
Negative control 1: ab280756 at a 1/80 dilution followed by ab150080 at a 1/200 dilution.
Negative control 2: ab179513 at a 1/200 dilution followed by ab150113 at a 1/1000 dilution.
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Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized NIH/3T3 (Mouse embryonic fibroblast, Left) / PC-12 (Rat adrenal gland pheochromocytoma, Right) cells labelling Mouse IgG2c with ab280756 at 1/1000 dilution (0.1µg) (Red) compared with a Mouse IgG2c, kappa monoclonal [18C8BC7AD10] - Isotype Control (ab170191, Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti mouse IgG (Alexa Fluor® 488, ab150113) at 1/2000 dilution was used as the secondary antibody.
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Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized PC-12 cells labelling Mouse IgG2c with ab280756 at 1/80 dilution, followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594)at 1/500 dilution (Red). The nuclear counterstain was DAPI (Blue).
Confocal image showing no staining in PC-12 cells.
Negative control 1: ab280756 at a 1/80 dilution followed by ab150080 at a 1/200 dilution.
Negative control 2: ab179513 at a 1/200 dilution followed by ab150113 at a 1/1000 dilution.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab280756 尚未被引用在任何文献中。