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小鼠IgG2b [PLPV219] -同型对照(ab91366)

Q&A (1)References (5)
Flow Cytometry - Mouse IgG2b [PLPV219] - Isotype Control (ab91366)

    Conjugates logo Related conjugates and formulations

    APC CF405M FITC PE

    概述

    • 产品名称

      小鼠IgG2b [PLPV219] -同型对照
      参阅全部 Mouse 亚类对照

    • 特异性

      ab91366 was produced against a synthetic hapten, which is normally not present in Humans or animals. Therefore, ab91366 does not react with any antigen of Human or animal cells.

    • 经测试应用

      适用于: Flow Cytmore details

    • 免疫原

      Vegetal protein

    • 常规说明

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    • 形式

      Liquid

    • 存放说明

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.

    • 存储溶液

      pH: 7.20
      Preservative: 0.1% Sodium azide
      Constituents: PBS, 1% BSA
    • Concentration information loading...

    • 纯度

      Protein G purified

    • 克隆

      单克隆

    • 克隆编号

      PLPV219

    • 同种型

      IgG2b

    • 研究领域

    • 别名

      • Mouse Isotype Control

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab91366于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    Flow Cyt
    Use 2µg for 106 cells. ab91366 is a negative isotype control
    说明
    Flow Cyt
    Use 2µg for 106 cells. ab91366 is a negative isotype control

    图片

    • Flow Cytometry - Mouse IgG2b [PLPV219] - Isotype Control (ab91366)
      Flow Cytometry - Mouse IgG2b [PLPV219] - Isotype Control (ab91366)

      Overlay histogram showing HeLa cells stained with ab54944 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab54944, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

    文献 (5)

    发表研究结果有使用 ab91366？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab91366 被引用在 5 文献中.

    • Cao Y  et al. ER stress-induced mediator C/EBP homologous protein thwarts effector T cell activity in tumors through T-bet repression. Nat Commun 10:1280 (2019). PubMed: 30894532
    • Guo HH  et al. Liver-target nanotechnology facilitates berberine to ameliorate cardio-metabolic diseases. Nat Commun 10:1981 (2019). PubMed: 31040273
    • Huang X  et al. Defining the NSD2 interactome: PARP1 PARylation reduces NSD2 histone methyltransferase activity and impedes chromatin binding. J Biol Chem 294:12459-12471 (2019). PubMed: 31248990
    • Abo Al Hayja M  et al. Reduced expression of peroxisome proliferator-activated receptor alpha in BAL and blood T cells of non-löfgren's sarcoidosis patients. J Inflamm (Lond) 12:28 (2015). Flow Cyt ; Human . PubMed: 25969669
    • Carilho Torrao RB  et al. Healthy ageing and depletion of intracellular glutathione influences T cell membrane thioredoxin-1 levels and cytokine secretion. Chem Cent J 7:150 (2013). Flow Cyt . PubMed: 24007191

    客户评价及客户问答

    Q&A
    提交问题

    Question


    I am using human PBMCs and human bronchoalveolar cells and I am using flow cytometry for analysing the cells.

    I bought recently these antibodies:

    The primary ab2779 PPAR alpha antibody [3B6/PPAR]

    The secondary Ab DyLight® 488 goat anti-mouse IgG (H+L) (ab96879)


    Isotype Control: ab91366 Mouse IgG2b [PLPV219]


    Now I would like to expand my experiment and analyse PPAR-delta and PPAR-gamma in the same cells and I will use flow cytometry.

    My questions:

    1- Which PPAR-delta Ab should I use? My suggestion is PPAR-A ab58137.

    2- Can I use the same isotype? That is ab91366 Mouse IgG2b [PLPV219]?

    3- Can I use the same secondary Ab? That is DyLight® 488 goat anti-mouse IgG (H+L) (ab96879)

    4- Which PPAR-gamma Ab should I use? My suggestion is Ab41928.

    5- Can I use the same isotype? That is ab91366 Mouse IgG2b [PLPV219]?

    6- Can I use the same secondary Ab? That is DyLight® 488 goat anti-mouse IgG (H+L) (ab96879)

    Read More
    Answer

    Thank you for contacting us.
    Regarding PPAR-delta ab:
    I think ab58137 (www.abcam.com/ab58137) as you suggested would be the best antibody to try as it has been tested in flow cyt and using the same secondary ab (ab96879) would be fine. However you would have to use a mouse IgG1 as a control as the primary antibody (ab58137) has the isotype IgG1. I suggest isotype control antibody, mouse IgG1 [ICIGG1] ab91353 (www.abcam.com/ab91353).
    Regarding PPAR-gamma ab, we do not have any antibodies which have been tested in flow cytometry.
    However, if you would be willing to test one of these antibodies and providing us with your results, whether positive or negative, I could give you a discount.
    Simply follow these easy steps below to apply for our AbTrial Program:
    1. Reply to this email, letting us know you are interested in testing this product.
    2. Our scientists will email you an inactive personal discount code for the value of the product.
    3. Purchase and test the product at the regular price.
    4. Submit your results, including your discount code in the additional notes section of your Abreview.
    5. Once the Abreview is submitted, the discount code will become active.
    6. Apply your discount code on your next order to receive that value off.
    Please let me know if you have any questions about this offer and I would be happy to help you further.
    The Terms and Conditions of this offer can be found at: www.abcam.com/abtrial.
    If you wish to test the antibody you suggested, ab41928, you could use the same secondary antibody but you would need a mouse IgG2a as a control. I suggest ab91361 as an isotype control (www.abcam.com/ab91361).
    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

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