小鼠IgG1，Kappa单克隆抗体[B11/6] -同型对照(ab91353)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control (ab91353)](https://www.abcam.cn/ps/products/91/ab91353/Images/ab91353-319806-mouse-igg1-kappa-monoclonal-b11-6-isotype-control-immunohistochemistry-formalin-paraffin.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control (ab91353)")
概述
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产品名称
小鼠IgG1，Kappa单克隆抗体[B11/6] -同型对照
参阅全部 Mouse 亚类对照 -
经测试应用
适用于: Flow Cyt, IHC-P, IHC-Frmore details -
免疫原
Other Immunogen Type corresponding to Mouse. Synthetic hapten
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常规说明
ab91353 enables an estimation of non-specific binding of mouse monoclonal antibodies to cell surface components in peripheral blood and tissue. Suitable for whole blood, Ficoll-separated preparations, frozen and paraffin embedded sections
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.09% Sodium azide
Constituent: 1% BSA -
Concentration information loading... -
纯度
Protein G purified -
纯化说明
ab91353 is purified and passed through a 0.22µm filter -
Isotype control说明
ab91353 enables an estimation of non-specific binding of mouse monoclonal antibodies to cell surface components in peripheral blood and tissue. Suitable for whole blood, Ficoll-separated preparations, frozen and paraffin embedded sections -
克隆
单克隆 -
克隆编号
B11/6 -
同种型
IgG1 -
轻链类型
kappa -
研究领域
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别名
- Mouse Isotype Control
相关产品
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Alternative Versions
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Compatible Secondaries
应用
Our Abpromise guarantee covers the use of ab91353 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
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| Flow Cyt | Use 2µg for 106 cells. ab91353 is a negative isotype control |
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| IHC-P | Use at an assay dependent concentration. | |
| IHC-Fr | Use at an assay dependent concentration. |
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control (ab91353)Karanovic et al One. 2016 Aug 25;11(8):e0161706. doi: 10.1371/journal.pone.0161706. eCollection 2016. Fig 9. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/
Glomerular nestin expression in experimental groups.
(A) Negative control (×200).
(C) SHADR group: Diffuse glomerular nestin expression was detected involving almost all podocytes within glomerulus. After losartan and tempol treatment, either single or in combination, kidneys restored nestin expression similar to controls (SHC group).
Immunostaining was applied on 5 μm thick paraffin sections. After deparaffinization and rehydration, the sections were treated by microwave for 20 minutes at 400 W in citrate buffer (pH 6.0). After antigen retrieval, samples were incubated for 1 hour at room temperature with primary antibody for nestin (dilution 1:100). Sections were then treated using 3-amino-9-ethylcarbazole (AEC) as substrate, and counterstained with hematoxylin. Negative controls were performed by omitting the first antibody and mouse monoclonal antibodies as isotype control mouse IgG1 (ab91353) antibody was also used.
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![Flow Cytometry - Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control (ab91353)](https://www.abcam.cn/ps/products/91/ab91353/Images/ab91353-319805-mouse-igg1-kappa-monoclonal-b11-6-isotype-control-flow-cytometry.jpg)
Flow Cytometry - Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control (ab91353)Hirakawa et al PLoS One. 2016 Aug 3;11(8):e0159912. doi: 10.1371/journal.pone.0159912. eCollection 2016. Fig 1. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/Effects of hypoxia on mRNA expression of IGF1R and IGF1, and production of IGF1 in cancer cells.
Cell surface expression of IGF1R by FACScan analysis. IGF1R expression level of Panc-1, RWP-1, OCUP-AT, and MiaPaCa-2 cells was higher in hypoxia than that in normoxia.
Cells (2 x 106 cells/mL) were fixed with 2% paraformaldehyde and incubated in PBS with anti IGF1R antibody (ab16890, Abcam) or mouse IgG1- isotype control (ab91353, Abcam) for 30 minutes at 22°C. Cells were subsequently labeled with FITC-conjugated secondary antibody (1:500; ab96879, Abcam) for 30 minutes at 22°C.
The percentage of positive cells were calculated and compared with isotype-matched control-stained cells.
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![Flow Cytometry - Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control (ab91353)](https://www.abcam.cn/ps/products/91/ab91353/Images/ab91353-273296-mouse-igg1-kappa-monoclonal-b11-6-isotype-control-flow-cytometry.jpg)
Flow Cytometry - Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control (ab91353)Overlay histogram showing HeLa (Human epithelial cell line from cervix adenocarcinoma) cells stained with ab59509 (red line).
The cells were fixed with 80% methanol (5 minutes) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab59509, 1 µg/1x106 cells) for 30 minutes at 22ºC. The secondary antibody used was a goat anti-mouse DyLight® 488 (IgG, H+L) (ab96879) at 1/500 dilution for 30 minutes at 22ºC.
Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2 µg/1x106 cells) used under the same conditions.
Acquisition of >5,000 events was performed.
实验方案
文献 (28)
ab91353 被引用在 28 文献中.
- Kobayashi M et al. Inhibition of autophagy in theca cells induces CYP17A1 and PAI-1 expression via ROS/p38 and JNK signalling during the development of polycystic ovary syndrome. Mol Cell Endocrinol 508:110792 (2020). PubMed: 32199904
- Li Y et al. Effect of integrin ß1 in the treatment of stress urinary incontinence by electrical stimulation. Mol Med Rep 19:4727-4734 (2019). PubMed: 31059065
- Rusu P et al. GPD1 Specifically Marks Dormant Glioma Stem Cells with a Distinct Metabolic Profile. Cell Stem Cell 25:241-257.e8 (2019). PubMed: 31303549
- Mills R et al. Intrapulmonary Autoantibodies to HSP72 Are Associated with Improved Outcomes in IPF. J Immunol Res 2019:1845128 (2019). PubMed: 31098385
- Setthawong P et al. Generation of porcine induced-pluripotent stem cells from Sertoli cells. Theriogenology 127:32-40 (2019). PubMed: 30639694
