小鼠IgG1,kappa单克隆抗体[15-6E10A7] -同型对照(Alexa Fluor® 594) (ab178000)

概述

  • 产品名称

    小鼠IgG1,kappa单克隆抗体[15-6E10A7] -同型对照(Alexa Fluor® 594)
    参阅全部 IgG 亚类对照
  • 偶联物

    Alexa Fluor® 594. Ex: 590nm, Em: 617nm
  • 经测试应用

    适用于: ICC/IFmore details
  • 常规说明

    Isotype controls are used to confirm that the primary antibody binding is specific and not a result of non-specific Fc receptor binding or other protein interactions. The isotype control antibody should match the primary antibody’s host species, isotype, and possible conjugation. The Control performed appropriately in all materials and platforms that were tested. 

    Product was previously marketed under the MitoSciences sub-brand.

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C. Store In the Dark.
  • 存储溶液

    Preservative: 0.02% Sodium azide
    Constituents: 68% PBS, 30% Glycerol, 1% BSA
  • Concentration information loading...
  • 纯度

    Ammonium Sulphate Precipitation
  • 克隆

    单克隆
  • 克隆编号

    15-6E10A7
  • 同种型

    IgG1
  • 轻链类型

    kappa
  • 研究领域

  • 细胞定位

    Secreted
  • 别名

    • Ig gamma 1 chain C region
    • Ig gamma 2 chain C region
    • Ig gamma 3 chain C region
    • Ig gamma 4 chain C region
    • IgG
    • IGHG1
    • IGHG2
    • IGHG3
    • IGHG4
    • Immunoglobin heavy constant gamma 1
    • Immunoglobulin G
    see all

应用

Our Abpromise guarantee covers the use of ab178000 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration.

图片

  • Immunocytochemistry data for ab178000. All panels show HeLa cells stained with DAPI as well as; a no primary control (A), ab178000 at 0.1 μg/mL (B), an Alexa 594 anti-SDHA conjugated antibody (ab170172) at 0.1 μg/mL (C).

    Cells were PFA fixed and stored in 0.02%azide/PBS in 4°C, then blocked with 10%GS for 2 hours, primary was diluted in 10%GS and incubated for 2 hours, cells were washed 3x with 1%GS and DAPI was added for 10 minutes in 1%GS.

文献

ab178000 has not yet been referenced specifically in any publications.

客户评价及客户问答

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