小鼠IgG1，kappa单克隆抗体[15-6E10A7] -同型对照(ab170190)
概述
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产品名称小鼠IgG1，kappa单克隆抗体[15-6E10A7] -同型对照
参阅全部 Coiled-coil domain-containing protein 113 亚类对照 -
特异性This Mouse IgG1 Isotype Control Antibody was raised in mouse against a yeast-specific protein making it unsuitable for yeast experiments.
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经测试应用适用于: Flow Cyt, ICC, IHC-Pmore details
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免疫原
Other Immunogen Type. ab170190 binds specifically to DNA-binding domain of the yeast GAL4 protein transcription activator.
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常规说明
This antibody clone is manufactured by Abcam.
Isotype controls are used to confirm that the primary antibody binding is specific and not a result of non-specific Fc receptor binding or other protein interactions. The isotype control antibody should match the primary antibody’s host species, isotype, and possible conjugation. The control performed appropriately in all materials and platforms that were tested.
Product was previously marketed under the MitoSciences sub-brand.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
性能
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形式Liquid
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存放说明Shipped at 4°C. Store at +4°C.
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存储溶液Preservative: 0.02% Sodium azide
Constituent: HEPES buffered saline -
Concentration information loading... -
纯度Ammonium Sulphate Precipitation
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克隆单克隆
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克隆编号15-6E10A7
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同种型IgG1
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轻链类型kappa
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别名
- CCDC113
- Coiled-coil domain-containing protein 113 Gene names
- DKFZp434N1418
- HSPC065
相关产品
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Alternative Versions
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Compatible Secondaries
应用
Our Abpromise guarantee covers the use of ab170190 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt | Use a concentration of 1 µg/ml. | |
| ICC | Use a concentration of 1 µg/ml. | |
| IHC-P | Use at an assay dependent concentration. PubMed: 26627833 |
图片
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![Flow Cytometry - Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)](https://www.abcam.cn/ps/products/170/ab170190/Images/ab170190-171551-mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-flow-cytometry.jpg)
Flow Cytometry - Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)Flow cytometry experiments with 4% PFA fixed HeLa (Human adenocarcinoma), 653s (mouse myeloma), H4IIE (rat hepatoma) and H9C2 (rat myoblast) were performed with the IgG1 Isotype Control Antibody (red) and no primary antibody negative control (black).
An Alexa Fluor® 488 conjugate with isotype specificity to the mouse antibody was used as a secondary antibody. The isotype control at 1 ug/mL shows no higher signal than the no primary negative control.
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![Immunocytochemistry - Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)](https://www.abcam.cn/ps/products/170/ab170190/Images/ab170190-171550-mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-immunocytochemistry.jpg)
Immunocytochemistry - Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)Immunocytochemistry (ICC) experiments with NIH-3T3 (mouse sarcoma), COS7 (monkey kidney fibroblast), H9C2 (rat myoblast) and Hela (human adenocarcinoma) were performed with the IgG1 Isotype Control Antibody (top), no primary antibody negative control (middle), and ab14705 as a positive control (bottom).
An Alexa Fluor® 488 conjugate with isotype specificity to the mouse antibody was used as a secondary antibody. The isotype control at 1 ug/mL shows no higher signal than the no primary negative control.
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![ELISA - Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)](https://www.abcam.cn/ps/products/170/ab170190/Images/ab170190-169713-mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-elisa.jpg)
ELISA - Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)Fluorescence ELISA.
An isotyping ELISA was performed by coating a 96-well plate with 1.25 ug/mL of the IgG1 Isotype Control Antibody and detecting with Alexa Fluor conjugates specific to mouse IgG1, IgG2a, IgG2b, IgG3, IgM and heavy and light chains (H&L) of IgG. This experiment verifies that the primary antibody's isotype is correct and that it is successfully bound by the secondary antibody.
实验方案
文献
This product has been referenced in:
- Sun Q et al. PEGylated TiO2 nanoparticles mediated inhibition of cell migration via integrin beta 1. Sci Technol Adv Mater 19:271-281 (2018). Read more (PubMed: 29707067) »
- Dudka D et al. Complete microtubule-kinetochore occupancy favours the segregation of merotelic attachments. Nat Commun 9:2042 (2018). Read more (PubMed: 29795284) »
