小鼠IgG1,kappa单克隆抗体[15-6E10A7] -同型对照(ab170190)
概述
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产品名称
小鼠IgG1,kappa单克隆抗体[15-6E10A7] -同型对照
参阅全部 Mouse 亚类对照 -
特异性
This Mouse IgG1 Isotype Control Antibody was raised in mouse against a yeast-specific protein making it unsuitable for yeast experiments. -
经测试应用
适用于: Flow Cyt, ICC, IHC-Pmore details -
免疫原
Other Immunogen Type. ab170190 binds specifically to DNA-binding domain of the yeast GAL4 protein transcription activator.
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常规说明
This antibody clone is manufactured by Abcam.
Isotype controls are used to confirm that the primary antibody binding is specific and not a result of non-specific Fc receptor binding or other protein interactions. The isotype control antibody should match the primary antibody’s host species, isotype, and possible conjugation. The control performed appropriately in all materials and platforms that were tested.
Product was previously marketed under the MitoSciences sub-brand.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
Preservative: 0.02% Sodium azide
Constituent: HEPES buffered saline -
Concentration information loading...
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纯度
Ammonium Sulphate Precipitation -
克隆
单克隆 -
克隆编号
15-6E10A7 -
同种型
IgG1 -
轻链类型
kappa -
研究领域
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别名
- Mouse Isotype Control
相关产品
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Alternative Versions
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Compatible Secondaries
应用
Our Abpromise guarantee covers the use of ab170190 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用 | Ab评论 | 说明 |
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Flow Cyt | Use a concentration of 1 µg/ml. | |
ICC | Use a concentration of 1 µg/ml. | |
IHC-P | Use at an assay dependent concentration. PubMed: 26627833 |
图片
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Flow cytometry experiments with 4% PFA fixed HeLa (Human adenocarcinoma), 653s (mouse myeloma), H4IIE (rat hepatoma) and H9C2 (rat myoblast) were performed with the IgG1 Isotype Control Antibody (red) and no primary antibody negative control (black).
An Alexa Fluor® 488 conjugate with isotype specificity to the mouse antibody was used as a secondary antibody. The isotype control at 1 ug/mL shows no higher signal than the no primary negative control.
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Immunocytochemistry (ICC) experiments with NIH-3T3 (mouse sarcoma), COS7 (monkey kidney fibroblast), H9C2 (rat myoblast) and Hela (human adenocarcinoma) were performed with the IgG1 Isotype Control Antibody (top), no primary antibody negative control (middle), and ab14705 as a positive control (bottom).
An Alexa Fluor® 488 conjugate with isotype specificity to the mouse antibody was used as a secondary antibody. The isotype control at 1 ug/mL shows no higher signal than the no primary negative control.
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Fluorescence ELISA.
An isotyping ELISA was performed by coating a 96-well plate with 1.25 ug/mL of the IgG1 Isotype Control Antibody and detecting with Alexa Fluor conjugates specific to mouse IgG1, IgG2a, IgG2b, IgG3, IgM and heavy and light chains (H&L) of IgG. This experiment verifies that the primary antibody's isotype is correct and that it is successfully bound by the secondary antibody.
实验方案
文献 (10)
ab170190 被引用在 10 文献中.
- Rao F et al. Exosomes from Human Gingiva-Derived Mesenchymal Stem Cells Combined with Biodegradable Chitin Conduits Promote Rat Sciatic Nerve Regeneration. Stem Cells Int 2019:2546367 (2019). PubMed: 31191669
- Chen X et al. Oxidative stress-induced IL-15 trans-presentation in keratinocytes contributes to CD8+ T cells activation via JAK-STAT pathway in vitiligo. Free Radic Biol Med 139:80-91 (2019). PubMed: 31078730
- Sun Q et al. PEGylated TiO2 nanoparticles mediated inhibition of cell migration via integrin beta 1. Sci Technol Adv Mater 19:271-281 (2018). PubMed: 29707067
- Dudka D et al. Complete microtubule-kinetochore occupancy favours the segregation of merotelic attachments. Nat Commun 9:2042 (2018). PubMed: 29795284
- Deng H et al. Susceptibility of epithelial cells cultured from different regions of human cervix to HPV16-induced immortalization. PLoS One 13:e0199761 (2018). PubMed: 29944714
- Xu JG et al. TGF-ß1-induced differentiation of SHED into functional smooth muscle cells. Stem Cell Res Ther 8:10 (2017). PubMed: 28114966
- Cui Y et al. The miR-20-Rest-Wnt signaling axis regulates neural progenitor cell differentiation. Sci Rep 6:23300 (2016). Flow Cyt . PubMed: 26996236
- Landa-Solís C et al. Cryopreserved CD90+ cells obtained from mobilized peripheral blood in sheep: a new source of mesenchymal stem cells for preclinical applications. Cell Tissue Bank N/A:N/A (2015). PubMed: 26220398
- Ebisawa M et al. Significance of caveolin-1 and matrix metalloproteinase 14 gene expression in canine mammary tumours. Vet J N/A:N/A (2015). IF . PubMed: 26364240
- Douglas DN et al. Oxidative stress attenuates lipid synthesis and increases mitochondrial fatty acid oxidation in hepatoma cells infected with hepatitis C virus. J Biol Chem N/A:N/A (2015). IHC-P . PubMed: 26627833