Key features and details
- Sensitivity: 1.5 ng/ml
- Range: 2.3 ng/ml - 150 ng/ml
- Sample type: Cell culture supernatant
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
参阅全部 IGF2 试剂盒
样品类型Cell culture supernatant
灵敏度< 1.5 ng/ml
范围2.3 ng/ml - 150 ng/ml
实验步骤Multiple steps standard assay
Abcam’s IGF2 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse IGF2 in cell culture supernatants.
This assay employs an antibody specific for mouse IGF2 coated on a 96-well plate. Standards and samples are pipetted into the wells and IGF2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse IGF2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IGF2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Please note that ab100696 has only been validated with cell culture supernatant samples and cannot be used with plasma samples. It is also not validated for serum samples.
存放说明Store at -20°C. Please refer to protocols.
组件 1 x 96 tests 1,000X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer 1 x 25ml 5X Assay Diluent 1 x 15ml Biotinylated anti-Mouse IGF2 2 vials IGF2 Microplate (12 x 8 wells) 1 unit Recombinant Mouse IGF2 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml
功能The insulin-like growth factors possess growth-promoting activity. In vitro, they are potent mitogens for cultured cells. IGF-II is influenced by placental lactogen and may play a role in fetal development.
Preptin undergoes glucose-mediated co-secretion with insulin, and acts as physiological amplifier of glucose-mediated insulin secretion. Exhibits osteogenic properties by increasing osteoblast mitogenic activity through phosphoactivation of MAPK1 and MAPK3.
疾病相关Epigenetic changes of DNA hypomethylation in IGF2 are a cause of Silver-Russell syndrome (SIRS) [MIM:180860]. SIRS is a clinically heterogeneous condition characterized by severe intrauterine growth retardation, poor postnatal growth, craniofacial features such as a triangular shaped face and a broad forehead, body asymmetry, and a variety of minor malformations.
序列相似性Belongs to the insulin family.
翻译后修饰O-glycosylated with a core 1 or possibly core 8 glycan.
- Information by UniProt
- IGF 2
- IGF II
ab100696 被引用在 5 文献中.
- Park KS et al. Loss of imprinting mutations define both distinct and overlapping roles for misexpression of IGF2 and of H19 lncRNA. Nucleic Acids Res 45:12766-12779 (2017). PubMed: 29244185
- Patel HJ et al. Structure-activity relationship in a purine-scaffold compound series with selectivity for the endoplasmic reticulum Hsp90 paralog Grp94. J Med Chem 58:3922-43 (2015). PubMed: 25901531
- Ferrón SR et al. Differential genomic imprinting regulates paracrine and autocrine roles of IGF2 in mouse adult neurogenesis. Nat Commun 6:8265 (2015). PubMed: 26369386
- Moore SF et al. Loss of the insulin receptor in murine megakaryocytes/platelets causes thrombocytosis and alterations in IGF signalling. Cardiovasc Res 107:9-19 (2015). PubMed: 25902782
- Patel PD et al. Paralog-selective Hsp90 inhibitors define tumor-specific regulation of HER2. Nat Chem Biol 9:677-84 (2013). Sandwich ELISA . PubMed: 23995768