小鼠ICAM 1 ELISA试剂盒(ab252355)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 28.15 pg/ml
- Range: 62.5 pg/ml - 4000 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum, Tissue Culture Media
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
概述
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产品名称
小鼠ICAM 1 ELISA试剂盒
参阅全部 ICAM1 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Serum 8 4.6% 批次间 样品 n Mean SD CV% Serum 3 4% -
样品类型
Cell culture supernatant, Serum, Tissue Culture Media, Hep Plasma, EDTA Plasma, Cit plasma -
检测类型
Sandwich (quantitative) -
灵敏度
28.15 pg/ml -
范围
62.5 pg/ml - 4000 pg/ml -
回收率
特定样本回收率 样品类型 平均% 范围 Cell culture supernatant 113 107% - 115% Serum 96 81% - 111% Tissue Extracts 91 82% - 99% Hep Plasma 91 84% - 100% EDTA Plasma 99 90% - 104% Cit plasma 102 86% - 113% -
检测时间
1h 30m -
实验步骤
One step assay -
种属反应性
与反应: Mouse
不与反应: Cow -
产品概述
Mouse ICAM 1 ELISA Kit (ab252355) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of ICAM 1 protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, serum, and tissue culture media. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse ICAM 1 with 28.15 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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说明
ICAM1, is a cell surface glycoprotein typically expressed in endothelial cells and cells of the immune system. The extracellular portion of ICAM-1 forms five immunoglobulin like domains attached to a single hydrophobic transmembrane region and a short cytoplasmic tail. ICAM-1, binds to the Leukocyte Integrins LFA-1 (CD11a/CD18) and Mac-1 (CD11b/CD18) as well as to non-integrin ligands such as CD43, fibrinogen, hyaluronan, Rhinoviruses and Plasmodium falciparum-infected erythrocytes. Binding to LFA-1 facilitates trans-endothelial leukocyte migration to areas of inflammation via promotion of endothelial apical cups assembly. Mouse ICAM1 shares 92.6 % and 48.6 % sequence homology with rat and human ICAM1, respectively.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
平台
Pre-coated microplate (12 x 8 well strips)
性能
-
存放说明
Store at +4°C. Please refer to protocols. -
组件 1 x 96 tests 10X Mouse ICAM 1 Capture Antibody 1 x 600µl 10X Mouse ICAM 1 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 5BR 1 x 6ml Mouse ICAM 1 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
研究领域
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功能
ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. In case of rhinovirus infection acts as a cellular receptor for the virus. -
序列相似性
Belongs to the immunoglobulin superfamily. ICAM family.
Contains 5 Ig-like C2-type (immunoglobulin-like) domains. -
翻译后修饰
Monoubiquitinated, which is promoted by MARCH9 and leads to endocytosis. -
细胞定位
Membrane. - Information by UniProt
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别名
- Antigen identified by monoclonal antibody BB2
- BB 2
- BB2
see all -
数据库链接
- Entrez Gene: 15894 Mouse
- SwissProt: P13597 Mouse
- Unigene: 435508 Mouse
相关产品
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Alternative Versions
图片
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Standard Curve comparison between human Mouse ICAM 1 SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.
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The ICAM 1 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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The ICAM 1 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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The concentrations of ICAM 1 were measured in duplicates, interpolated from the ICAM 1 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1:200, plasma (citrate) 1:200, plasma (EDTA) 1:200 and plasma (heparin) 1:200. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean ICAM 1 concentration was determined to be 506.72 ng/mL in serum, 535.54 ng/mL in plasma (citrate), 498.48 ng/mL in plasma (EDTA), 563.77 ng/mL in and plasma (heparin).
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The concentrations of ICAM 1 were measured in duplicates, interpolated from the ICAM 1 standard curves and corrected for sample dilution. Undiluted samples are as follows: LPS stimulated J774A.1 cell culture supernatant 50%, unstimulated J774A.1 cell culture supernatant 50%, and spleen cell culture supernatant 4%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean ICAM 1 concentration was determined to be 4.59 ng/mL in LPS stimulated J774A.1 cell culture supernatant, 3.23 ng/mL in unstimulated J774A.1 cell culture supernatant, and 10.94 ng/mL in and spleen cell culture supernatant.
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The concentrations of ICAM 1 were measured in duplicate and interpolated from the ICAM 1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean ICAM 1 concentration was determined to be 1341.51 pg/mL in spleen extract and 3625 pg/mL in lung extract.
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To learn more about the advantages of recombinant antibodies see here.
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SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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To learn more about the advantages of SimpleStep ELISA® kits see here.
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab252355 被引用在 4 文献中.
- Zou J et al. Effects of Diosmin on Vascular Leakage and Inflammation in a Mouse Model of Venous Obstruction. Front Nutr 9:831485 (2022). PubMed: 35273990
- Guo Z et al. Diosmin Alleviates Venous Injury and Muscle Damage in a Mouse Model of Iliac Vein Stenosis. Front Cardiovasc Med 8:785554 (2021). PubMed: 35097005
- Hu T et al. USP38 might be a new therapeutic target for glioma via regulation of cancer cell metastasis. Folia Neuropathol 59:359-371 (2021). PubMed: 35114776
- Cheng CF et al. Adipocyte browning and resistance to obesity in mice is induced by expression of ATF3. Commun Biol 2:389 (2019). PubMed: 31667363