概述

  • 产品名称
    小鼠HSP60 ELISA试剂盒
    参阅全部 HSP60 试剂盒
  • 检测方法
    Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    NIH/3T3 5 6.8%
    批次间
    样品 n Mean SD CV%
    NIH/3T3 3 12.5%
  • 样品类型
    Cell culture extracts, Tissue Extracts
  • 检测类型
    Sandwich (quantitative)
  • 灵敏度
    5.1 pg/ml
  • 范围
    46.875 pg/ml - 3000 pg/ml
  • 回收率

    特定样本回收率
    样品类型 平均% 范围
    Serum 98 81.2% - 110.2%
    Cell culture media 92.3 91.2% - 93%

  • 检测时间
    1h 30m
  • 实验步骤
    One step assay
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 产品概述

    Abcam’s HSP60 in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of HSP60 protein in mouse cell and tissue extract samples.

     

    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.

  • 说明

    HSP60 is mitochondrial matrix protein functioning in protein folding and macromolecular assembly. HSP60 may also prevent miss-folding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix.

  • 经测试应用
    适用于: Sandwich ELISAmore details
  • 平台
    Pre-coated microplate (12 x 8 well strips)

性能

应用

Our Abpromise guarantee covers the use of ab208344 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

图片

  • Background-subtracted data values (mean +/- SD) are graphed.

  • The concentrations of HSP60 were measured in duplicate and interpolated from the HSP60 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean HSP60 concentration was determined to be 3,456 pg/mL in C2C12 cell extract sample and 1,347 pg/mL in C6 cell extract sample.

  • The concentrations of HSP60 were measured in three different dilutions in duplicate and interpolated from the HSP60 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted in ng of HSP60 per mg of extract (mean +/- SD, n=3).

实验方案

文献

ab208344 has not yet been referenced specifically in any publications.

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