产品名称小鼠heart组织裂解物- total蛋白(14 days)
参阅全部 Heart 细胞裂解液
常规说明Source: Outbred NIH-Swiss Albino mice (14 days old)
The mouse heart tissue was collected from fourteen (14) day-old mice. The lysate was prepared by homogenization in modified RIPA buffer (50 mM Tris -HCl, pH 7.4, 1% Triton X-100, 0.2% sodium deoxycholate, 0.2% sodium dodecylsulfate (SDS), 1 mM sodium ethylenediaminetetraacetate, 1 mM phenylmethylsulfonyl flouride, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris -HCl pH 6.8, 12.5% glycerol, 1% SDS, 0.01% bromophenol blue) containing 5% b-mercaptoethanol.
经测试应用适用于: WBmore details
存放说明Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
Constituents: 5% Beta mercaptoethanol, 12.5% Glycerol, 1% SDS, 1% Triton-X-100, 1% Sodium Deoxycholate, 0.01% Bromophenol blue, 5µg/ml Leupeptin, 5µg/ml Aprotinin, 50mM Tris HCl, 150mM Sodium chloride, 1mM EDTA, 1mM PMSF, pH 6.8
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Our Abpromise guarantee covers the use of ab7194 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent dilution. Ready to load; 10-20 µg/lane is recommended for mini gel.|
ab7194 has not yet been referenced specifically in any publications.