Key features and details
- Sensitivity: 6 pg/ml
- Range: 2.74 pg/ml - 2000 pg/ml
- Sample type: Cell culture supernatant, Plasma
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
参阅全部 GM-CSF 试剂盒
样品类型Cell culture supernatant, Plasma
灵敏度< 6 pg/ml
范围2.74 pg/ml - 2000 pg/ml
特定样本回收率 样品类型 平均% 范围 Cell culture supernatant 102.53 88% - 108% Plasma 100.32 89% - 107%
实验步骤Multiple steps standard assay
Abcam’s GM-CSF Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse GM-CSF in plasma and cell culture supernatants.
This assay employs an antibody specific for mouse GM-CSF coated on a 96- well plate. Standards and samples are pipetted into the wells and GM-CSF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse GM-CSF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of GM-CSF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
We have not been able to detect endogenous Mouse GM-CSF in normal serum with ab100685, only in serum spiked with Mouse GM-CSF.
Get higher sensitivity in only 90 minutes with Mouse GM-CSF ELISA Kit (CSF2) (ab201276) from our SimpleStep ELISA® range.
存放说明Store at -20°C. Please refer to protocols.
组件 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer 1 x 25ml 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Biotinylated anti-Mouse GM-CSF 2 vials GM-CSF Microplate (12 x 8 wells) 1 unit Recombinant Mouse GM-CSF Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml
功能Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes.
序列相似性Belongs to the GM-CSF family.
- Information by UniProt
- Colony stimulating factor
- Colony Stimulating Factor 2
- Colony stimulating factor 2 (granulocyte-macrophage)
SimpleStep ELISA kits