Key features and details
- Sensitivity: 0.5 pg/ml
- Range: 0.62 pg/ml - 150 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
参阅全部 G-CSF 试剂盒
样品类型Cell culture supernatant, Serum, Plasma
灵敏度< 0.5 pg/ml
范围0.62 pg/ml - 150 pg/ml
特定样本回收率 样品类型 平均% 范围 Cell culture supernatant 97.52 84% - 104% Serum 95.53 83% - 104% Plasma 94.28 82% - 102%
实验步骤Multiple steps standard assay
Abcam’s G-CSF Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse G-CSF in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for mouse G-CSF coated on a 96- well plate. Standards and samples are pipetted into the wells and G-CSF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse G-CSF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of G-CSF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
存放说明Store at -20°C. Please refer to protocols.
组件 1 x 96 tests 20X Wash Buffer 1 x 25ml 5X Assay Diluent B 1 x 15ml 80x HRP-Streptavidin Concentrate 1 x 200µl Assay Diluent A 1 x 30ml Biotinylated anti-Mouse G-CSF 2 vials G-CSF Microplate (12 x 8 wells) 1 unit Recombinant Mouse G-CSF Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml
相关性Granulocyte Colony Stimulating Factor (G-CSF) a cytokine that controls the production, differentiation, and function of granulocytes. It is a potent stimulator of bone marrow cells especially those of neutrophil lineage. In addition, G-CSF can enhance the survival and activate the immunological functions of mature neutrophils. Recombinant human G-CSF has been developed by pharmaceutical companies, and since the late 1980's, multiple clinical trials have explored its efficacy in a variety of conditions.
- Colony stimulating factor 3 (granulocyte)
- CSF 3