小鼠FX ELISA试剂盒(total FX antigen) (ab272779)
Key features and details
- Range: 2.5 ng/ml - 500 ng/ml
- Sample type: Cit plasma, Other biological fluids
- Detection method: Colorimetric
- Assay type: Quantitative
- Reacts with: Mouse
概述
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产品名称
小鼠FX ELISA试剂盒(total FX antigen)
参阅全部 Factor X 试剂盒 -
检测方法
Colorimetric -
样品类型
Other biological fluids, Cit plasma -
检测类型
Quantitative -
范围
2.5 ng/ml - 500 ng/ml -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Mouse -
产品概述
Mouse FX ELISA kit (total FX antigen) (ab272779) is for the quantitative determination of total Factor X and Xa in biological fluids.
Mouse Factor X will bind to the affinity purified capture antibody coated on the microtiter plate. Factor X, Xa, and Xa in complex with inhibitors will react with the antibody on the plate. After appropriate washing steps, biotin labeled polyclonal anti-mouse Factor X primary antibody binds to the Factor X. Excess antibody is washed away and bound polyclonal antibody is then reacted with Streptavidin conjugated to horseradish peroxidase. Following an additional washing step, TMB substrate is used for color development at 450nm. The amount of color development is directly proportional to the concentration of total Factor X in the sample.
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平台
Microplate
性能
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存放说明
Store at +4°C. Please refer to protocols. -
组件 1 x 96 tests 10X Wash Buffer 1 x 50ml Anti-Mouse FX Primary Antibody Lyophilized Vial 1 vial Mouse FX ELISA Plate 1 unit Mouse FX Standard Lyophilized Vial 1 vial Streptavidin-HRP Secondary Reagent 1 vial TMB Substrate 1 x 10ml -
研究领域
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功能
Factor Xa is a vitamin K-dependent glycoprotein that converts prothrombin to thrombin in the presence of factor Va, calcium and phospholipid during blood clotting. -
组织特异性
Plasma; synthesized in the liver. -
疾病相关
Defects in F10 are the cause of factor X deficiency (FA10D) [MIM:227600]. A hemorrhagic disease with variable presentation. Affected individuals can manifest prolonged nasal and mucosal hemorrhage, menorrhagia, hematuria, and occasionally hemarthrosis. Some patients do not have clinical bleeding diathesis. -
序列相似性
Belongs to the peptidase S1 family.
Contains 2 EGF-like domains.
Contains 1 Gla (gamma-carboxy-glutamate) domain.
Contains 1 peptidase S1 domain. -
翻译后修饰
The vitamin K-dependent, enzymatic carboxylation of some glutamate residues allows the modified protein to bind calcium.
N- and O-glycosylated.
The activation peptide is cleaved by factor IXa (in the intrinsic pathway), or by factor VIIa (in the extrinsic pathway).
The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains. -
细胞定位
Secreted. - Information by UniProt
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别名
- Activated factor Xa heavy chain
- Coagulation factor X
- F10
see all -
数据库链接
- Entrez Gene: 14058 Mouse
- SwissProt: O88947 Mouse
- Unigene: 262589 Mouse
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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