小鼠FGF-21 ELISA试剂盒,Fluorescent (ab229382)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 1 pg/ml
- Range: 1.2 pg/ml - 4800 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Fluorescent
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
概述
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产品名称
小鼠FGF-21 ELISA试剂盒,Fluorescent
参阅全部 FGF21 试剂盒 -
检测方法
Fluorescent -
精确度
批次内 样品 n Mean SD CV% Serum 5 3% 批次间 样品 n Mean SD CV% Serum 3 4% -
样品类型
Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
检测类型
Sandwich (quantitative) -
灵敏度
1 pg/ml -
范围
1.2 pg/ml - 4800 pg/ml -
回收率
特定样本回收率 样品类型 平均% 范围 Cell culture supernatant 94 91% - 97% Serum 103 99% - 107% Hep Plasma 94 88% - 104% EDTA Plasma 100 92% - 108% Cit plasma 101 94% - 106% -
检测时间
1h 30m -
实验步骤
One step assay -
种属反应性
与反应: Mouse
不与反应: Cow -
产品概述
FGF-21 in vitro CatchPoint® SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of FGF-21 protein in mouse serum, plasma, and cell culture supernatants.
This CatchPoint SimpleStep ELISA kit has been optimized for Molecular Devices Microplate Readers. Click here for a list of recommended Microplate Readers.
If using a Molecular Devices’ plate reader supported by SoftMax® Pro software, a preconfigured protocol for these CatchPoint SimpleStep ELISA Kits is available with all the protocol and analysis settings at www.softmaxpro.org
The CatchPoint SimpleStep ELISA employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. CatchPoint HRP Development Solution containing the Stoplight Red Substrate is added. During incubation, the substrate is catalyzed by HRP generating a fluorescent product. Signal is generated proportionally to the amount of bound analyte and the intensity is measured in a fluorescence plater reader at 530/570/590 nm Excitation/Cutoff/Emission.
Rat serum and plasma samples are not compatible with this kit.
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说明
FGF-21 is a secreted protein, a member of a large FGF gene family. FGF-21 stimulates glucose uptake in differentiated adipocytes via the induction of glucose transporter SLC2A1/GLUT1 expression but not via SLC2A4/GLUT4 expression. FGF-21 activity probably requires the presence of KLB.
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平台
Pre-coated microplate (12 x 8 well strips)
性能
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存放说明
Store at +4°C. Please refer to protocols. -
组件 1 x 96 tests 100X Stoplight Red Substrate 1 x 120µl 10X Mouse FGF-21 Capture Antibody 1 x 600µl 10X Mouse FGF-21 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 500X Hydrogen Peroxide (H2O2, 3%) 1 x 50µl Antibody Diluent CPR 1 x 6ml Mouse FGF-21 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated Black 96-Well Microplate 1 unit Stoplight Red Substrate Buffer 1 x 12ml -
研究领域
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功能
Stimulates glucose uptake in differentiated adipocytes via the induction of glucose transporter SLC2A1/GLUT1 expression (but not SLC2A4/GLUT4 expression). Activity requires the presence of KLB. -
序列相似性
Belongs to the heparin-binding growth factors family. -
细胞定位
Secreted. - Information by UniProt
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别名
- FGF 21
- FGF-21
- Fgf21
see all -
数据库链接
- Entrez Gene: 56636 Mouse
- SwissProt: Q9JJN1 Mouse
- Unigene: 143736 Mouse
图片
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SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Background-subtracted data values (mean +/- SD) are graphed.
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The concentrations of FGF-21 were measured in duplicates, interpolated from the FGF-21 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 100%, plasma (citrate) 100%, plasma (EDTA) 100%, and plasma (heparin) 50%. The interpolated dilution factor (to neat sample) corrected values are plotted (mean +/- SD, n=2). The mean FGF-21 concentration was determined to be 409 pg/mL in neat serum, 397 pg/mL in neat plasma (citrate), 358 pg/mL in neat plasma (EDTA), and 348 pg/mL in neat plasma (heparin).
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The concentrations of FGF-21 were measured in duplicates, interpolated from the FGF-21 standard curves and corrected for sample dilution. Undiluted samples are as follows: L-929 cell culture supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean FGF-21 concentration was determined to be 59 pg/mL in neat L-929 cell culture supernatant.
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The concentrations of FGF-21 were measured in three different dilutions in duplicate and interpolated from the FGF-21 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted in pg FGF-21 per mL of neat serum (mean +/- SD, n=3). FGF-21 concentration was determined to be 401 pg/mg in normal mouse serum, and 9,193 pg/mL in pregnant mouse serum.
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To learn more about the advantages of recombinant antibodies see here.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab229382 被引用在 1 文献中.
- Hope DCD et al. Hypoaminoacidemia underpins glucagon-mediated energy expenditure and weight loss. Cell Rep Med 3:100810 (2022). PubMed: 36384093